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Literature DB >> 24226941

A simple, versatile feeder layer system for Brassica oleracea protoplast culture.

Abstract

Protoplasts from cauliflower (Brassica oleracea ssp. botrytis) and broccoli (ssp. italica) leaves and hypocotyls were successfully cultured on membrane filters over a feeder layer of cells from a B. campestris suspension culture. Cells from rice, tomato and tobacco suspensions were not as effective as the B. campestris cells. Plants were recovered from protoplasts of previously recalcitrant Brassica genotypes. Protoplasts cultured in low numbers (10-100) on the feeder layer divided and formed colonies capable of plant regeneration, as did fused protoplasts.

Entities: Species

Year: 1990 PMID： 24226941 DOI： 10.1007/BF00232859

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

11 in total

1. A rapid and efficient alternative procedure for the regeneration of plants from hypocotyl protoplasts of Brassica napus.

Authors: T L Barsby; S A Yarrow; J F Shepard
Journal: Plant Cell Rep Date: 1986-04 Impact factor: 4.570

2. Plant regeneration from leaf protoplasts of Brassica oleracea var. italica CV Green Comet broccoli.

Authors: D Robertson; E D Earle
Journal: Plant Cell Rep Date: 1986-02 Impact factor: 4.570

3. Haploid plants from pollen grains.

Authors: J P Nitsch; C Nitsch
Journal: Science Date: 1969-01-03 Impact factor: 47.728

4. Nutrient requirements of suspension cultures of soybean root cells.

Authors: O L Gamborg; R A Miller; K Ojima
Journal: Exp Cell Res Date: 1968-04 Impact factor: 3.905

5. A simple culture method for Brassica hypototyl protoplasts.

Authors: P V Chuong; K P Pauls; W D Beversdorf
Journal: Plant Cell Rep Date: 1985-02 Impact factor: 4.570

6. Effect of Chilling Temperatures upon Cell Cultures of Tomato.

Authors: F M Dupont; L C Staraci; B Chou; B R Thomas; B G Williams; J B Mudd
Journal: Plant Physiol Date: 1985-01 Impact factor: 8.340

7. Transfer and segregation of triazine tolerant chloroplasts in Brassica napus L.

Authors: J E Thomzik; R Hain
Journal: Theor Appl Genet Date: 1988-08 Impact factor: 5.699

8. Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana.

Authors: R Fluhr; D Aviv; M Edelman; E Galun
Journal: Theor Appl Genet Date: 1983-06 Impact factor: 5.699

9. Plant regeneration from mesophyll protoplast culture of cabbage (Brassica oleracea var 'capitata').

Authors: Y Y Fu; S R Jia; Y Lin
Journal: Theor Appl Genet Date: 1985-12 Impact factor: 5.699

10. The introduction of CMS mitochondria to triazine tolerant Brassica napus L., var. "Regent", by micromanipulation of individual heterokaryons.

Authors: S A Yarrow; S C Wu; T L Barsby; R J Kemble; J F Shepard
Journal: Plant Cell Rep Date: 1986-12 Impact factor: 4.570

10 in total

1. Protoplast-to-plant regeneration in cotton (Gossypium hirsutum L. cv. Coker 312) using feeder layers.

Authors: M C Peeters; K Willems; R Swennen
Journal: Plant Cell Rep Date: 1994-01 Impact factor: 4.570

2. Synthesis of Ogura male sterile rapeseed (Brassica napus L.) with cold tolerance by protoplast fusion and effects of atrazine resistance on seed yield.

Authors: D W Heath; E D Earle
Journal: Plant Cell Rep Date: 1996-09 Impact factor: 4.570

A simple, versatile feeder layer system for Brassica oleracea protoplast culture.

1. A rapid and efficient alternative procedure for the regeneration of plants from hypocotyl protoplasts of Brassica napus.

2. Plant regeneration from leaf protoplasts of Brassica oleracea var. italica CV Green Comet broccoli.

3. Haploid plants from pollen grains.

4. Nutrient requirements of suspension cultures of soybean root cells.

5. A simple culture method for Brassica hypototyl protoplasts.

6. Effect of Chilling Temperatures upon Cell Cultures of Tomato.

7. Transfer and segregation of triazine tolerant chloroplasts in Brassica napus L.

8. Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana.

9. Plant regeneration from mesophyll protoplast culture of cabbage (Brassica oleracea var 'capitata').

10. The introduction of CMS mitochondria to triazine tolerant Brassica napus L., var. "Regent", by micromanipulation of individual heterokaryons.

1. Protoplast-to-plant regeneration in cotton (Gossypium hirsutum L. cv. Coker 312) using feeder layers.

2. Synthesis of Ogura male sterile rapeseed (Brassica napus L.) with cold tolerance by protoplast fusion and effects of atrazine resistance on seed yield.

3. Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters.

4. Transfer of resistance to Xanthomonas campestris pv campestris into Brassica oleracea L. by protoplast fusion.

5. Protoplast fusion-derived Ogura male sterile cauliflower with cold tolerance.

6. Organellar segregation, rearrangement and recombination in protoplast fusion-derived Brassica oleracea calli.

7. Novel flowering and fatty acid characters in rapid cycling Brassica napus L. resynthesized by protoplast fusion.

8. Improvement of protoplast culture protocols for Beta vulgaris L. (sugar beet).

9. Regeneration of plants from protoplasts of rapid cycling Brassica oleracea L.

10. An alginate-layer technique for culture of Brassica oleracea L. protoplasts.