Literature DB >> 24243275

Fluorescence intensity and anisotropy decay of the 4',6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation.

J R Lakowicz1, I Gryczynski.   

Abstract

We examined the emission spectra, intensity decays, and anisotropy decays of the DNA-4',6-diamidino-2-phenylindole (DAPI) complex resulting from one- and two-photon excitation of fluorescence. Similar lifetimes and correlation times were recovered from the frequency-domain data. However, the initial anisotropies of DAPI for one- and two-photon excitation revealed different angles between the absorption and the emission oscillators, 17.8 and 23.8°, respectively. This suggests the presence of two overlapping transitions in DAPI with different one- and two-photon cross sections for absorption.

Entities:  

Year:  1992        PMID： 24243275     DOI： 10.1007/BF00867671

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  15 in total

1.  Fluorescence lifetime imaging of free and protein-bound NADH.

Authors:  J R Lakowicz; H Szmacinski; K Nowaczyk; M L Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1992-02-15       Impact factor: 11.205

2.  Two-photon spectroscopy of locked-11-cis-rhodopsin: evidence for a protonated Schiff base in a neutral protein binding site.

Authors:  R R Birge; L P Murray; B M Pierce; H Akita; V Balogh-Nair; L A Findsen; K Nakanishi
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

3.  Fluorescent complexes of DNA with DAPI 4',6-diamidine-2-phenyl indole.2HCl or DCI 4',6-dicarboxyamide-2-phenyl indole.

Authors:  J Kapuściński; B Skoczylas
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

4.  Two-photon laser scanning fluorescence microscopy.

Authors:  W Denk; J H Strickler; W W Webb
Journal:  Science       Date:  1990-04-06       Impact factor: 47.728

5.  Resolution of complex anisotropy decays by variable frequency phase-modulation fluorometry: a stimulation study.

Authors:  B P Maliwal; J R Lakowicz
Journal:  Biochim Biophys Acta       Date:  1986-09-26

6.  DNA-4'-6-diamidine-2-phenylindole interactions: a comparative study employing fluorescence and ultraviolet spectroscopy.

Authors:  M L Barcellona; R Favilla; J von Berger; M Avitabile; N Ragusa; L Masotti
Journal:  Arch Biochem Biophys       Date:  1986-10       Impact factor: 4.013

7.  Fluorescence lifetime distributions of DNA-4',6-diamidino-2-phenylindole complex.

Authors:  M L Barcellona; E Gratton
Journal:  Biochim Biophys Acta       Date:  1989-12-08

8.  Interactions of 4', 6-diamidine-2-phenylindole with synthetic polynucleotides.

Authors:  J Kapuściński; W Szer
Journal:  Nucleic Acids Res       Date:  1979-08-10       Impact factor: 16.971

9.  The fluorescence properties of a DNA probe. 4'-6-Diamidino-2-phenylindole (DAPI).

Authors:  M L Barcellona; E Gratton
Journal:  Eur Biophys J       Date:  1990       Impact factor: 1.733

10.  Optical Studies of the interaction of 4'-6'-diamidino-2-phenylindole with DNA and metaphase chromosomes.

Authors:  M S Lin; D E Comings; O S Alfi
Journal:  Chromosoma       Date:  1977-03-07       Impact factor: 4.316
View more
  12 in total

1.  Emerging biomedical and advanced applications of time-resolved fluorescence spectroscopy.

Authors:  J R Lakowicz; P A Koen; H Szmacinski; I Gryczynski; J Kuśba
Journal:  J Fluoresc       Date:  1994-03       Impact factor: 2.217

2.  Fluorescence intensity and anisotropy decays of the DNA stain Hoechst 33342 resulting from one-photon and two-photon excitation.

Authors:  I Gryczynski; J R Lakowicz
Journal:  J Fluoresc       Date:  1994-12       Impact factor: 2.217

3.  Fluorescence of horse liver alcohol dehydrogenase using one- and two-photon excitation.

Authors:  J R Lakowicz; B Kierdaszuk; I Gryczynski; H Malak
Journal:  J Fluoresc       Date:  1996-03       Impact factor: 2.217

4.  Effects of laser polarization on responses of the fluorescent Ca2+ indicator X-Rhod-1 in neurons and myelin.

Authors:  Ileana Micu; Craig Brideau; Li Lu; Peter K Stys
Journal:  Neurophotonics       Date:  2017-06-05       Impact factor: 3.593

5.  Fluorescence Spectral Properties of 2,5-Diphenyl-1,3,4-oxadiazole with Two-Color Two-Photon Excitation.

Authors:  Joseph R Lakowicz; Ignacy Gryczynski; Henryk Malak; Zygmunt Gryczynski
Journal:  J Phys Chem       Date:  1996-12-12

6.  ADVANCES IN FLUORESCENCE SPECTROSCOPY: MULTI-PHOTON EXCITATION, ENGINEERED PROTEINS, MODULATION SENSING AND MICROSECOND RHENIUM METAL-LIGAND COMPLEXES.

Authors:  J R Lakowicz; I Gryczynski; L Tolosa; J D Dattelbaum; F N Castellano; L Li; G Rao
Journal:  Acta Phys Pol A       Date:  1999-01       Impact factor: 0.577

7.  Two-photon excitation of dioxane: time-resolved measurements of excited state complex formation with water.

Authors:  J R Lakowicz; I Gryczynski; K Nowaczyk
Journal:  Spectrochim Acta A Mol Biomol Spectrosc       Date:  1997-09       Impact factor: 4.098

8.  Light quenching of tetraphenylbutadiene fluorescence observed during two-photon excitation.

Authors:  I Gryczynski; V Bogdanov; J R Lakowicz
Journal:  J Fluoresc       Date:  1993-06       Impact factor: 2.217

Review 9.  Fluorescence anisotropy imaging in drug discovery.

Authors:  Claudio Vinegoni; Paolo Fumene Feruglio; Ignacy Gryczynski; Ralph Mazitschek; Ralph Weissleder
Journal:  Adv Drug Deliv Rev       Date:  2018-02-02       Impact factor: 15.470

10.  Three-photon excitation ofp-quaterphenyl with a mode-locked femtosecond Ti:sapphire laser.

Authors:  I Gryczynski; H Malak; J R Lakowicz
Journal:  J Fluoresc       Date:  1996-09       Impact factor: 2.217
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.