Literature DB >> 24226997

Fluorescence of horse liver alcohol dehydrogenase using one- and two-photon excitation.

J R Lakowicz1, B Kierdaszuk, I Gryczynski, H Malak.   

Abstract

We examined the steady-state and time-resolved emission of liver alcohol dehydrogenase resulting from one-photon and two-photon excitation. Previous studies with one-photon excitation revealed that the two nonidentical tryptophan residues display different emission spectra and decay times. The use of two-photon excitation resulted in similar emission spectra, multiexponential intensity decays, time-resolved emission spectra, and anisotropy decays as was observed for one-photon excitation. These results suggest that both nonidentical tryptophan residues are excited to a similar extent for one- and two-photon excitation. However, the limiting anisotropy (r 0) with two-photon excitation from 585 to 610 nm is below 0.1 and appears distinct from that observed previously forN-acetyl-L-tryptophanamide.

Entities:  

Year:  1996        PMID： 24226997     DOI： 10.1007/BF00726726

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  28 in total

1.  ZINC, A COMPONENT OF YEAST ALCOHOL DEHYDROGENASE.

Authors:  B L Vallee; F L Hoch
Journal:  Proc Natl Acad Sci U S A       Date:  1955-06-15       Impact factor: 11.205

2.  Fluorescence intensity and anisotropy decay of the 4',6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation.

Authors:  J R Lakowicz; I Gryczynski
Journal:  J Fluoresc       Date:  1992-06       Impact factor: 2.217

3.  Nanosecond time-resolved emission spectroscopy of a fluorescence probe adsorbed to L-alpha-egg lecithin vesicles.

Authors:  J H Easter; R P DeToma; L Brand
Journal:  Biophys J       Date:  1976-06       Impact factor: 4.033

4.  Two-photon spectroscopy of locked-11-cis-rhodopsin: evidence for a protonated Schiff base in a neutral protein binding site.

Authors:  R R Birge; L P Murray; B M Pierce; H Akita; V Balogh-Nair; L A Findsen; K Nakanishi
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

5.  Two-photon laser scanning fluorescence microscopy.

Authors:  W Denk; J H Strickler; W W Webb
Journal:  Science       Date:  1990-04-06       Impact factor: 47.728

6.  Resolution of complex anisotropy decays by variable frequency phase-modulation fluorometry: a stimulation study.

Authors:  B P Maliwal; J R Lakowicz
Journal:  Biochim Biophys Acta       Date:  1986-09-26

7.  Resolution of mixtures of fluorophores using variable-frequency phase and modulation data.

Authors:  E Gratton; M Limkeman; J R Lakowicz; B P Maliwal; H Cherek; G Laczko
Journal:  Biophys J       Date:  1984-10       Impact factor: 4.033

8.  Rotational freedom of tryptophan residues in proteins and peptides.

Authors:  J R Lakowicz; B P Maliwal; H Cherek; A Balter
Journal:  Biochemistry       Date:  1983-04-12       Impact factor: 3.162

9.  Structure of a triclinic ternary complex of horse liver alcohol dehydrogenase at 2.9 A resolution.

Authors:  H Eklund; J P Samma; L Wallén; C I Brändén; A Akeson; T A Jones
Journal:  J Mol Biol       Date:  1981-03-15       Impact factor: 5.469

10.  The mechanism of quenching of liver alcohol dehydrogenase fluorescence due to ternary complex formation.

Authors:  W R Laws; J D Shore
Journal:  J Biol Chem       Date:  1978-12-10       Impact factor: 5.157
View more
  2 in total

1.  Fluorescence anisotropy of tyrosinate anion using one-, two- and three-photon excitation: tyrosinate anion fluorescence.

Authors:  Borys Kierdaszuk
Journal:  J Fluoresc       Date:  2012-12-12       Impact factor: 2.217

2.  Interpretation of fluorescence decays using a power-like model.

Authors:  Jakub Włodarczyk; Borys Kierdaszuk
Journal:  Biophys J       Date:  2003-07       Impact factor: 4.033
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.