Literature DB >> 24241753

Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS.

R Töpfer1, M Pröls, J Schell, H H Steinbiß.   

Abstract

The reporter genes for Chloramphenicolacetyltransferase (CAT), Neomycinphosphotransferase-(NPT)-II and β-Glucuronidase (GUS) were compared in transient gene expression experiments in tobacco mesophyll protoplasts. For this purpose, nearly identical chimeric genes controlled by the CaMV 35 S promoter were constructed. The detection level of each system was determined yielding the following order of relative sensitivity: CAT<NPT II<GUS.

Entities:  

Year:  1988        PMID： 24241753     DOI： 10.1007/BF00272529

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  17 in total

1.  Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays.

Authors:  P R Ebert; S B Ha; G An
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

2.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

3.  A set of plant expression vectors for transcriptional and translational fusions.

Authors:  R Töpfer; V Matzeit; B Gronenborn; J Schell; H H Steinbiss
Journal:  Nucleic Acids Res       Date:  1987-07-24       Impact factor: 16.971

4.  The gapped duplex DNA approach to oligonucleotide-directed mutation construction.

Authors:  W Kramer; V Drutsa; H W Jansen; B Kramer; M Pflugfelder; H J Fritz
Journal:  Nucleic Acids Res       Date:  1984-12-21       Impact factor: 16.971

5.  Selection-expression plasmid vectors for use in genetic transformation of higher plants.

Authors:  J Velten; J Schell
Journal:  Nucleic Acids Res       Date:  1985-10-11       Impact factor: 16.971

6.  Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

Authors:  C M Gorman; L F Moffat; B H Howard
Journal:  Mol Cell Biol       Date:  1982-09       Impact factor: 4.272

7.  Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species.

Authors:  R M Hauptmann; P Ozias-Akins; V Vasil; Z Tabaeizadeh; S G Rogers; R B Horsch; I K Vasil; R T Fraley
Journal:  Plant Cell Rep       Date:  1987-07       Impact factor: 4.570

8.  The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts.

Authors:  P H Schreier; E A Seftor; J Schell; H J Bohnert
Journal:  EMBO J       Date:  1985-01       Impact factor: 11.598

9.  High level expression of introduced chimaeric genes in regenerated transformed plants.

Authors:  J D Jones; P Dunsmuir; J Bedbrook
Journal:  EMBO J       Date:  1985-10       Impact factor: 11.598

10.  GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors:  R A Jefferson; T A Kavanagh; M W Bevan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
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  25 in total

1.  Transformation of cauliflower (Brassica oleracea L. var. botrytis)--an experimental survey.

Authors:  K Eimert; F Siegemund
Journal:  Plant Mol Biol       Date:  1992-06       Impact factor: 4.076

2.  Assaying synthetic ribozymes in plants: high-level expression of a functional hammerhead structure fails to inhibit target gene activity in transiently transformed protoplasts.

Authors:  L Mazzolini; M Axelos; N Lescure; P Yot
Journal:  Plant Mol Biol       Date:  1992-11       Impact factor: 4.076

3.  Detection of gene regulatory signals in plants revealed by T-DNA-mediated fusions.

Authors:  P R Fobert; B L Miki; V N Iyer
Journal:  Plant Mol Biol       Date:  1991-10       Impact factor: 4.076

4.  Agrobacterium-mediated genetic transformation of oilseed Brassica campestris: Transformation frequency is strongly influenced by the mode of shoot regeneration.

Authors:  A Mukhopadhyay; N Arumugam; P B Nandakumar; A K Pradhan; V Gupta; D Pental
Journal:  Plant Cell Rep       Date:  1992-09       Impact factor: 4.570

5.  Efficient regeneration of Brassica oleracea hypocotyl protoplasts and high frequency genetic transformation by direct DNA uptake.

Authors:  A Mukhopadhyay; R Töpfer; A K Pradhan; Y S Sodhi; H H Steinbiß; J Schell; D Pental
Journal:  Plant Cell Rep       Date:  1991-10       Impact factor: 4.570

6.  Direct analysis of RNA transcripts in electroporated carrot protoplasts.

Authors:  E E Murray; W G Buchholz; B Bowen
Journal:  Plant Cell Rep       Date:  1990-07       Impact factor: 4.570

7.  Transient expression of a lysine-rich vicilin gene ofVicia faba in barley endosperm detected by immunological tissue printing after particle bombardment.

Authors:  U Heim; R Manteuffel; H Bäumlein; H H Steinbiss; U Wobus
Journal:  Plant Cell Rep       Date:  1995-01       Impact factor: 4.570

8.  Visualisation of transgene expression at the single protoplast level.

Authors:  J L Jung; S Bouzoubaa; D Gilmer; G Hahne
Journal:  Plant Cell Rep       Date:  1992-07       Impact factor: 4.570

9.  Intron-dependent transient expression of the maize GapA1 gene.

Authors:  M Donath; R Mendel; R Cerff; W Martin
Journal:  Plant Mol Biol       Date:  1995-07       Impact factor: 4.076

10.  An unusual internal ribosomal entry site of inverted symmetry directs expression of a potato leafroll polerovirus replication-associated protein.

Authors:  Hannah Miriam Jaag; Lawrence Kawchuk; Wolfgang Rohde; Rainer Fischer; Neil Emans; Dirk Prüfer
Journal:  Proc Natl Acad Sci U S A       Date:  2003-06-30       Impact factor: 11.205
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