Literature DB >> 24221726

Efficient regeneration of Brassica oleracea hypocotyl protoplasts and high frequency genetic transformation by direct DNA uptake.

A Mukhopadhyay1, R Töpfer, A K Pradhan, Y S Sodhi, H H Steinbiß, J Schell, D Pental.   

Abstract

Efficient regeneration (80%) and high frequency genetic transformation (10-33%) were achieved by culturing protoplasts isolated from hypocotyl tissues of six day old Brassica oleracea seedlings and by subjecting these protoplasts to PEG mediated direct plasmid uptake. Three different plasmid vectors carrying marker genes for resistance to methotrexate (dhfr), hygromycin (hpt) and phosphinotricin (bar) were constructed and used for transformation. Large number of normal, fertile transformants were obtained with vectors carrying hpt and bar genes. No transformants could be regenerated for resistance to methotrexate as it severely suppressed shoot differentiation.

Entities:  

Year:  1991        PMID： 24221726     DOI： 10.1007/BF00232604

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  18 in total

1.  Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS.

Authors:  R Töpfer; M Pröls; J Schell; H H Steinbiß
Journal:  Plant Cell Rep       Date:  1988-06       Impact factor: 4.570

2.  Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts.

Authors:  H M Zhang; H Yang; E L Rech; T J Golds; A S Davis; B J Mulligan; E C Cocking; M R Davey
Journal:  Plant Cell Rep       Date:  1988-10       Impact factor: 4.570

3.  Transfer of hygromycin resistance into Brassica napus using total DNA of a transgenic B. nigra line.

Authors:  C Golz; F Köhler; O Schieder
Journal:  Plant Mol Biol       Date:  1990-09       Impact factor: 4.076

4.  The isolation, culture and regeneration of Petunia leaf protoplasts.

Authors:  E M Frearson; J B Power; E C Cocking
Journal:  Dev Biol       Date:  1973-07       Impact factor: 3.582

5.  Genetically transformed maize plants from protoplasts.

Authors:  C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal:  Science       Date:  1988-04-08       Impact factor: 47.728

6.  Expression of the 2S albumin from Bertholletia excelsa in Brassica napus.

Authors:  P Guerche; E R De Almeida; M A Schwarztein; E Gander; E Krebbers; G Pelletier
Journal:  Mol Gen Genet       Date:  1990-05

7.  Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts.

Authors:  B Damm; R Schmidt; L Willmitzer
Journal:  Mol Gen Genet       Date:  1989-05

8.  Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer.

Authors:  I Potrykus; J Paszkowski; M W Saul; J Petruska; R D Shillito
Journal:  Mol Gen Genet       Date:  1985

9.  Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors:  I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal:  Plant Mol Biol       Date:  1987-09       Impact factor: 4.076

10.  Production of kanamycin resistant rice tissues following DNA uptake into protoplasts.

Authors:  H Yang; H M Zhang; M R Davey; B J Mulligan; E C Cocking
Journal:  Plant Cell Rep       Date:  1988-10       Impact factor: 4.570
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  10 in total

1.  Agrobacterium-mediated genetic transformation of oilseed Brassica campestris: Transformation frequency is strongly influenced by the mode of shoot regeneration.

Authors:  A Mukhopadhyay; N Arumugam; P B Nandakumar; A K Pradhan; V Gupta; D Pental
Journal:  Plant Cell Rep       Date:  1992-09       Impact factor: 4.570

2.  Genetic transformation of Brassica nigra by agrobacterium based vector and direct plasmid uptake.

Authors:  V Gupta; G Lakshmi Sita; M S Shaila; V Jagannathan
Journal:  Plant Cell Rep       Date:  1993-05       Impact factor: 4.570

3.  Somatic hybrids with substitution type genomic configuration TCBB for the transfer of nuclear and organelle genes from Brassica tournefortii TT to allotetraploid oilseed crop B. carinata BBCC.

Authors:  A Mukhopadhyay; N Arumugam; A K Pradhan; H N Murthy; B S Yadav; Y S Sodhi; D Pental
Journal:  Theor Appl Genet       Date:  1994-09       Impact factor: 5.699

4.  Agrobacterium-mediated transformation of cauliflower: optimization of protocol and development of Bt-transgenic cauliflower.

Authors:  R Chakrabarty; N Viswakarma; S R Bhat; P B Kirti; B D Singh; V L Chopra
Journal:  J Biosci       Date:  2002-09       Impact factor: 1.826

5.  Intergeneric hybridization between Diplotaxis siettiana and crop brassicas for the production of alloplasmic lines.

Authors:  P B Nanda Kumar; K R Shivanna
Journal:  Theor Appl Genet       Date:  1993-02       Impact factor: 5.699

6.  Regeneration of plants from protoplasts of rapid cycling Brassica oleracea L.

Authors:  L N Hansen; E D Earle
Journal:  Plant Cell Rep       Date:  1994-03       Impact factor: 4.570

7.  Variation amongst Brassica juncea cultivars for regeneration from hypocotyl explants and optimization of conditions for Agrobacterium-mediated genetic transformation.

Authors:  D Pental; A K Pradhan; Y S Sodhi; A Mukhopadhyay
Journal:  Plant Cell Rep       Date:  1993-05       Impact factor: 4.570

8.  Synthesis of hexaploid (AABBCC) somatic hybrids: a bridging material for transfer of 'tour' cytoplasmic male sterility to different Brassica species.

Authors:  N Arumugam; A Mukhopadhyay; V Gupta; D Pental; A K Pradhan
Journal:  Theor Appl Genet       Date:  1996-05       Impact factor: 5.699

9.  Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata).

Authors:  T D Metz; R Dixit; E D Earle
Journal:  Plant Cell Rep       Date:  1995-12       Impact factor: 4.570

10.  An alginate-layer technique for culture of Brassica oleracea L. protoplasts.

Authors:  Agnieszka Kiełkowska; Adela Adamus
Journal:  In Vitro Cell Dev Biol Plant       Date:  2012-03-30       Impact factor: 2.252
  10 in total

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