Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Cloning a selected fragment from a human DNA 'fingerprint': isolation of an extremely polymorphic minisatellite.

Literature DB >> 2423969

Cloning a selected fragment from a human DNA 'fingerprint': isolation of an extremely polymorphic minisatellite.

Z Wong, V Wilson, A J Jeffreys, S L Thein.

Abstract

A large hypervariable DNA fragment from a human DNA fingerprint was purified by preparative gel electrophoresis and molecular cloning. The cloned fragment contained a 6.3 kb long minisatellite consisting of multiple copies of a 37 bp repeat unit. Each repeat contained an 11 bp copy of the "core" sequences, a putative recombination signal in human DNA. The cloned minisatellite hybridized to a single locus in the human genome. This locus is extremely polymorphic, with at least 77 different alleles containing 14 to 525 repeat units per allele being resolved in a sample of 79 individuals. All alleles except the shortest are rare and the resulting heterozygosity is very high (approximately 97%). Cloned minisatellites should therefore provide a panel of extremely informative locus-specific probes ideal for linkage analysis in man.

Entities: Gene Species

Mesh：

Substances：

Year: 1986 PMID： 2423969 PMCID： PMC311468 DOI： 10.1093/nar/14.11.4605

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

1. Elution of DNA from agarose gels after electrophoresis.

Authors: R Yang; J Lis; R Wu
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

2. The establishment of lymphoblastoid lines from adult and fetal human lymphoid tissue and its dependence on EBV.

Authors: K Nilsson; G Klein; W Henle; G Henle
Journal: Int J Cancer Date: 1971-11-15 Impact factor: 7.396

3. A gradient of sex linkage in the pseudoautosomal region of the human sex chromosomes.

Authors: F Rouyer; M C Simmler; C Johnsson; G Vergnaud; H J Cooke; J Weissenbach
Journal: Nature Date: 1986 Jan 23-29 Impact factor: 49.962

4. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

Authors: F R Blattner; B G Williams; A E Blechl; K Denniston-Thompson; H E Faber; L Furlong; D J Grunwald; D O Kiefer; D D Moore; J W Schumm; E L Sheldon; O Smithies
Journal: Science Date: 1977-04-08 Impact factor: 47.728

5. Highly variable regions of DNA flank the human alpha globin genes.

Authors: D R Higgs; S E Goodbourn; J S Wainscoat; J B Clegg; D J Weatherall
Journal: Nucleic Acids Res Date: 1981-09-11 Impact factor: 16.971

6. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.

Authors: G Dretzen; M Bellard; P Sassone-Corsi; P Chambon
Journal: Anal Biochem Date: 1981-04 Impact factor: 3.365

7. A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes.

Authors: W A Loenen; W J Brammar
Journal: Gene Date: 1980-08 Impact factor: 3.688

8. A highly polymorphic locus in human DNA.

Authors: A R Wyman; R White
Journal: Proc Natl Acad Sci U S A Date: 1980-11 Impact factor: 11.205

Review 9. Construction of a genetic linkage map in man using restriction fragment length polymorphisms.

Authors: D Botstein; R L White; M Skolnick; R W Davis
Journal: Am J Hum Genet Date: 1980-05 Impact factor: 11.025

10. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

55 in total

1. BamHI polymorphism of locus D2S44 in a West German population as revealed by VNTR probe YNH24.

Authors: L Henke; S Cleef; M Zakrzewska; J Henke
Journal: Int J Legal Med Date: 1990-12 Impact factor: 2.686

2. Characterisation of a boundary between satellite III and alphoid sequences on human chromosome 10.

Authors: M S Jackson; S E Mole; B A Ponder
Journal: Nucleic Acids Res Date: 1992-09-25 Impact factor: 16.971

3. Comparison of restriction endonucleases and sources of probes for their efficiency in detecting restriction fragment length polymorphisms in lettuce (Lactuca sativa L.).

Authors: B S Landry; R Kesseli; H Leung; R W Michelmore
Journal: Theor Appl Genet Date: 1987-09 Impact factor: 5.699

Review 4. Potential genetic functions of tandem repeated DNA sequence blocks in the human genome are based on a highly conserved "chromatin folding code".

Authors: P Vogt
Journal: Hum Genet Date: 1990-03 Impact factor: 4.132

5. Use of the polymerase chain reaction to detect spacer size heterogeneity in plant 5S-rRNA gene clusters and to locate such clusters in wheat (Triticum aestivum L.).

Authors: A V Cox; M D Bennett; T A Dyer
Journal: Theor Appl Genet Date: 1992-04 Impact factor: 5.699

6. Application of DNA fingerprints for cell-line individualization.

Authors: D A Gilbert; Y A Reid; M H Gail; D Pee; C White; R J Hay; S J O'Brien
Journal: Am J Hum Genet Date: 1990-09 Impact factor: 11.025

7. Nucleotide sequence and genomic organization of bird minisatellites.

Authors: U B Gyllensten; S Jakobsson; H Temrin; A C Wilson
Journal: Nucleic Acids Res Date: 1989-03-25 Impact factor: 16.971

8. Molecular characterization and clinical use of a polymorphic tandem repeat in an intron of the human alanine:glyoxylate aminotransferase gene.

Authors: C J Danpure; G M Birdsey; G Rumsby; M J Lumb; P E Purdue; J Allsop
Journal: Hum Genet Date: 1994-07 Impact factor: 4.132

9. PCR-based study of conserved and variable DNA sequences of Tritrichomonas foetus isolates from Saskatchewan, Canada.

Authors: D E Riley; B Wagner; L Polley; J N Krieger
Journal: J Clin Microbiol Date: 1995-05 Impact factor: 5.948

10. Loss of heterozygosity and K-ras gene mutations in gastric cancer.

Authors: G N Ranzani; B Renault; N S Pellegata; P Fattorini; E Magni; F Bacci; D Amadori
Journal: Hum Genet Date: 1993-10-01 Impact factor: 4.132