Literature DB >> 24234902

Fluorescence staining of yeast cells permeabilized by killer toxin K1: Determination of optimum conditions.

H Kurzweilová1, K Sigler.   

Abstract

Optimal assay conditions were established for the previously described method used to determine the activity ofSaccharomyces cerevisiae pore-forming killer toxin K1. The method is based on cell staining with bromocresol purple. Sensitive cells ofS. cerevisiae from the early exponential phase under nongrowth conditions and in the presence of glucose were the most convenient for determining the killer toxin activity. Maximum killing war reached when the suspension was buffered with 10 mM citrate-phosphate at pH 4.6.

Entities:  

Year:  1993        PMID： 24234902     DOI： 10.1007/BF00865270

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  5 in total

Review 1.  Yeast dsRNA viruses: replication and killer phenotypes.

Authors:  D J Tipper; M J Schmitt
Journal:  Mol Microbiol       Date:  1991-10       Impact factor: 3.501

2.  Studies on the nature of the killer factor produced by Saccharomyces cerevisiae.

Authors:  D R Woods; E A Bevan
Journal:  J Gen Microbiol       Date:  1968-04

3.  Mode of action of yeast toxins: energy requirement for Saccharomyces cerevisiae killer toxin.

Authors:  N Skipper; H Bussey
Journal:  J Bacteriol       Date:  1977-02       Impact factor: 3.490

4.  Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.

Authors:  H Kurzweilová; K Sigler
Journal:  Yeast       Date:  1993-11       Impact factor: 3.239

5.  Factors governing substrate-induced generation and extrusion of protons in the yeast Saccharomyces cerevisiae.

Authors:  K Sigler; A Knotková; A Kotyk
Journal:  Biochim Biophys Acta       Date:  1981-05-20
  5 in total
  3 in total

1.  Measurement of membrane potential in Saccharomyces cerevisiae by the electrochromic probe di-4-ANEPPS: effect of intracellular probe distribution.

Authors:  R Chaloupka; J Plásek; J Slavík; V Siglerová; K Sigler
Journal:  Folia Microbiol (Praha)       Date:  1997       Impact factor: 2.099

Review 2.  Yeast killer toxin K1 and its exploitation in genetic manipulations.

Authors:  V Vondrejs; B Janderová; L Valásek
Journal:  Folia Microbiol (Praha)       Date:  1996       Impact factor: 2.099

3.  Effect of killer toxin K1 on yeast membrane potential reported by the diS-C3(3) probe reflects strain- and physiological state-dependent variations.

Authors:  M Eminger; D Gásková; B Brodská; A Holoubek; N Stadler; K Sigler
Journal:  Folia Microbiol (Praha)       Date:  1999       Impact factor: 2.099
  3 in total

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