Literature DB >> 7509098

Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.

H Kurzweilová1, K Sigler.   

Abstract

A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5.2. The method was used to determine the activity of Saccharomyces cerevisiae pore-forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 7509098     DOI： 10.1002/yea.320091107

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  13 in total

Review 1.  Yeast killer systems.

Authors:  W Magliani; S Conti; M Gerloni; D Bertolotti; L Polonelli
Journal:  Clin Microbiol Rev       Date:  1997-07       Impact factor: 26.132

2.  Isolation, purification, and characterization of a killer protein from Schwanniomyces occidentalis.

Authors:  W B Chen; Y F Han; S C Jong; S C Chang
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

Review 3.  General and molecular microbiology and microbial genetics in the IM CAS.

Authors:  Jan Nešvera
Journal:  J Ind Microbiol Biotechnol       Date:  2010-11-18       Impact factor: 3.346

4.  Fluorescence staining of yeast cells permeabilized by killer toxin K1: Determination of optimum conditions.

Authors:  H Kurzweilová; K Sigler
Journal:  J Fluoresc       Date:  1993-12       Impact factor: 2.217

5.  Significance of the lag phase in K1 killer toxin action on sensitive yeast cells.

Authors:  H Kurzweilová; K Sigler
Journal:  Folia Microbiol (Praha)       Date:  1995       Impact factor: 2.099

Review 6.  Yeast killer toxin K1 and its exploitation in genetic manipulations.

Authors:  V Vondrejs; B Janderová; L Valásek
Journal:  Folia Microbiol (Praha)       Date:  1996       Impact factor: 2.099

7.  Monitoring of killer yeast populations in mixed cultures: influence of incubation temperature of microvinifications samples.

Authors:  Yolanda Paola Maturano; María Cristina Nally; María Eugenia Toro; Lucía Inés Castellanos de Figueroa; Mariana Combina; Fabio Vazquez
Journal:  World J Microbiol Biotechnol       Date:  2012-07-14       Impact factor: 3.312

8.  Development of the FUN-1 family of fluorescent probes for vacuole labeling and viability testing of yeasts.

Authors:  P J Millard; B L Roth; H P Thi; S T Yue; R P Haugland
Journal:  Appl Environ Microbiol       Date:  1997-07       Impact factor: 4.792

9.  Study of membrane potential changes of yeast cells caused by killer toxin K1.

Authors:  D Gásková; H Kurzweilová; B Denksteinová; P Herman; J Vecer; K Sigler; J Plásek; J Malínský
Journal:  Folia Microbiol (Praha)       Date:  1994       Impact factor: 2.099

10.  Kinetic studies of killer toxin K1 binding to yeast cells indicate two receptor populations.

Authors:  H Kurzweilová; K Sigler
Journal:  Arch Microbiol       Date:  1994       Impact factor: 2.552
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.