Literature DB >> 24227157

PEG- and electroporation-induced transformation in Nicotiana tabacum: influence of genotype on transformation frequencies.

S Tyagi1, B Spörlein, A K Tyagi, R G Herrmann, H U Koop.   

Abstract

Experimental parameters for direct gene transfer with recombinant DNA encoding neomycin phosphotransferase II (NPTII) under control of eukaryotic expression signals were established. The introduced gene was shown by the growth of transformants on media containing kanamycin, by genomic blotting and by assaying NPTII activity. Leaf protoplasts from three green genotypes of varieties xanthii and petit havanna, and from four plastome-encoded albino genotypes of Nicotiana tabacum were analyzed with respect to cell division kinetics and yield of kanamycin-tolerant colonies after direct gene transfer. No clear correlation was found between the time of onset of cell division and transformation frequency.

Entities:  

Year:  1989        PMID： 24227157     DOI： 10.1007/BF00288813

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  11 in total

1.  Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.

Authors:  S O Rogers; A J Bendich
Journal:  Plant Mol Biol       Date:  1985-03       Impact factor: 4.076

2.  Stable transformation of tobacco by electroporation: evidence for plasmid concatenation.

Authors:  C D Riggs; G W Bates
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

3.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

4.  A set of plant expression vectors for transcriptional and translational fusions.

Authors:  R Töpfer; V Matzeit; B Gronenborn; J Schell; H H Steinbiss
Journal:  Nucleic Acids Res       Date:  1987-07-24       Impact factor: 16.971

5.  Nutrient requirements of suspension cultures of soybean root cells.

Authors:  O L Gamborg; R A Miller; K Ojima
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905

6.  A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts.

Authors:  B Reiss; R Sprengel; H Will; H Schaller
Journal:  Gene       Date:  1984-10       Impact factor: 3.688

7.  Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.

Authors:  E Beck; G Ludwig; E A Auerswald; B Reiss; H Schaller
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

8.  Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors:  I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal:  Plant Mol Biol       Date:  1987-09       Impact factor: 4.076

9.  Stable transformation of moth bean Vigna aconitifolia via direct gene transfer.

Authors:  F Köhler; C Golz; S Eapen; H Kohn; O Schieder
Journal:  Plant Cell Rep       Date:  1987-07       Impact factor: 4.570

10.  The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts.

Authors:  P H Schreier; E A Seftor; J Schell; H J Bohnert
Journal:  EMBO J       Date:  1985-01       Impact factor: 11.598
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  9 in total

1.  Transfer of defined numbers of chloroplasts into albino protoplasts by subprotoplast/protoplast microfusion: chloroplasts can be "cloned", by using suitable plastome combinations or selective pressure.

Authors:  L Eigel; H U Koop
Journal:  Mol Gen Genet       Date:  1992-06

2.  PEG-mediated plastid transformation: a new system for transient gene expression assays in chloroplasts.

Authors:  B Spörlein; M Streubel; G Dahlfeld; P Westhoff; H U Koop
Journal:  Theor Appl Genet       Date:  1991-10       Impact factor: 5.699

3.  Effect of various irradiation treatments of plant protoplasts on the transformation rates after direct gene transfer.

Authors:  F Köhler; I Benediktsson; G Cardon; C S Andreo; O Schieder
Journal:  Theor Appl Genet       Date:  1990-05       Impact factor: 5.699

4.  Transient gene expression in electroporated banana (Musa spp., cv. 'Bluggoe', ABB group) protoplasts isolated from regenerable embryogenetic cell suspensions.

Authors:  L Sagi; S Remy; B Panis; R Swennen; G Volckaert
Journal:  Plant Cell Rep       Date:  1994-02       Impact factor: 4.570

5.  Lipofectin: direct gene transfer to higher plants using cationic liposomes.

Authors:  B Spörlein; H U Koop
Journal:  Theor Appl Genet       Date:  1991-11       Impact factor: 5.699

6.  Transfer of hygromycin resistance into Brassica napus using total DNA of a transgenic B. nigra line.

Authors:  C Golz; F Köhler; O Schieder
Journal:  Plant Mol Biol       Date:  1990-09       Impact factor: 4.076

7.  Transfer of defined numbers of chloroplasts into albino protoplasts using an improved subprotoplast/protoplast microfusion procedure: transfer of only two chloroplasts leads to variegated progeny.

Authors:  L Eigel; R Oelmüller; H U Koop
Journal:  Mol Gen Genet       Date:  1991-07

8.  Introduction of mRNA to plant protoplasts using polyethylene glycol.

Authors:  D R Gallie
Journal:  Plant Cell Rep       Date:  1993-12       Impact factor: 4.570

9.  Factors affecting PEG-mediated stable transformation of maize protoplasts.

Authors:  C L Armstrong; W L Petersen; W G Buchholz; B A Bowen; S L Sulc
Journal:  Plant Cell Rep       Date:  1990-10       Impact factor: 4.570
  9 in total

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