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Literature DB >> 24306565

Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.

Abstract

We have developed a DNA extraction procedure for milligram amounts of plant tissue. Yields ranged from 0.3-200 nanograms of DNA per milligram of tissue. The factors affecting yield are discussed. Fresh tissue, as well as herbarium specimens (22-118 years old) and mummified seeds and embryos (500 to greater than 44 600 years old) were used. All tissues attempted (57 types from 29 species) yielded measurable amounts of DNA. In no case tested was inhibition observed for restriction enzymes BamHI or EcoRI.

Year: 1985 PMID： 24306565 DOI： 10.1007/BF00020088

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

8 in total

Review 1. Nuclear dna amounts in angiosperms.

Authors: M D Bennett; J B Smith
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1976-05-27 Impact factor: 6.237

2. The Relationship between Satellite Deoxyribonucleic Acid, Ribosomal Ribonucleic Acid Gene Redundancy, and Genome Size in Plants.

Authors: J Ingle; J N Timmis; J Sinclair
Journal: Plant Physiol Date: 1975-03 Impact factor: 8.340

3. Chromatin-bound DNA-dependent RNA polymerase in developing pea cotyledons.

Authors: C A Cullis
Journal: Planta Date: 1976-01 Impact factor: 4.116

4. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.

Authors: G I Bell; L J DeGennaro; D H Gelfand; R J Bishop; P Valenzuela; W J Rutter
Journal: J Biol Chem Date: 1977-11-25 Impact factor: 5.157

Review 5. A survey of DNA content per cell and per chromosome of prokaryotic and eukaryotic organisms: some evolutionary considerations.

Authors: A H Sparrow; H J Price; A G Underbrink
Journal: Brookhaven Symp Biol Date: 1972

6. Multiplicity of ribosomal RNA genes in Vicia species with different nuclear DNA contents.

Authors: E P Maher; D P Fox
Journal: Nat New Biol Date: 1973-10-10

7. Rapid isolation of high molecular weight plant DNA.

Authors: M G Murray; W F Thompson
Journal: Nucleic Acids Res Date: 1980-10-10 Impact factor: 16.971

8. DNA sequences from the quagga, an extinct member of the horse family.

Authors: R Higuchi; B Bowman; M Freiberger; O A Ryder; A C Wilson
Journal: Nature Date: 1984 Nov 15-21 Impact factor: 49.962

8 in total

330 in total

1. Chloroplast protein and centrosomal genes, a tRNA intron, and odd telomeres in an unusually compact eukaryotic genome, the cryptomonad nucleomorph.

Authors: S Zauner; M Fraunholz; J Wastl; S Penny; M Beaton; T Cavalier-Smith; U G Maier; S Douglas
Journal: Proc Natl Acad Sci U S A Date: 2000-01-04 Impact factor: 11.205

2. Cloning, expression and N-terminal myristoylation of CpCPK1, a calcium-dependent protein kinase from zucchini (Cucurbita pepo L.).

Authors: M Ellard-Ivey; R B Hopkins; T J White; T L Lomax
Journal: Plant Mol Biol Date: 1999-01 Impact factor: 4.076

Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.

Review 1. Nuclear dna amounts in angiosperms.

2. The Relationship between Satellite Deoxyribonucleic Acid, Ribosomal Ribonucleic Acid Gene Redundancy, and Genome Size in Plants.

3. Chromatin-bound DNA-dependent RNA polymerase in developing pea cotyledons.

4. Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.

Review 5. A survey of DNA content per cell and per chromosome of prokaryotic and eukaryotic organisms: some evolutionary considerations.

6. Multiplicity of ribosomal RNA genes in Vicia species with different nuclear DNA contents.

7. Rapid isolation of high molecular weight plant DNA.

8. DNA sequences from the quagga, an extinct member of the horse family.

1. Chloroplast protein and centrosomal genes, a tRNA intron, and odd telomeres in an unusually compact eukaryotic genome, the cryptomonad nucleomorph.

2. Cloning, expression and N-terminal myristoylation of CpCPK1, a calcium-dependent protein kinase from zucchini (Cucurbita pepo L.).

3. Two iron-regulated cation transporters from tomato complement metal uptake-deficient yeast mutants.

4. Lariat formation and a hydrolytic pathway in plant chloroplast group II intron splicing.

5. Low glutelin content1: a dominant mutation that suppresses the glutelin multigene family via RNA silencing in rice.

6. The absence of TIR-type resistance gene analogues in the sugar beet (Beta vulgaris L.) genome.

7. The use of RAPD analysis of sugar-beet mitochondrial DNA in search of CMS genes.

8. Targeted site-directed mutagenesis of a heme oxygenase locus by gene replacement in the moss Ceratodon purpureus.

9. An improved protocol for Agrobacterium-mediated transformation of Antirrhinum majus L.

10. Analysis of cytoplasmic genomes in somatic hybrids between navel orange (Citrus sinensis Osb.) and 'Murcott' tangor.