Literature DB >> 6295884

Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.

E Beck, G Ludwig, E A Auerswald, B Reiss, H Schaller.   

Abstract

The nucleotide sequence of 1200 bp from the unique region of transposon Tn5 containing the neomycin phosphotransferase gene (neo) was determined, and the location of the neo gene was identified by deletion mutants in a translational reading frame of 792 bp. The derived gene product, an aminoglycoside 3'-phosphotransferase (APH) II, consists of 264 amino acid residues and has a calculated Mr of 29053. Its amino acid sequence shows sequence homologies to the APH type I enzyme coded for by transposon Tn903 (Oka et al., 1981).

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Year:  1982        PMID： 6295884     DOI： 10.1016/0378-1119(82)90023-3

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  310 in total

1.  Sequences downstream of the translation initiation codon are important determinants of translation efficiency in chloroplasts.

Authors:  H Kuroda; P Maliga
Journal:  Plant Physiol       Date:  2001-01       Impact factor: 8.340

2.  Selection of functional tRNA primers and primer binding site sequences from a retroviral combinatorial library: identification of new functional tRNA primers in murine leukemia virus replication.

Authors:  A H Lund; M Duch; F S Pedersen
Journal:  Nucleic Acids Res       Date:  2000-02-01       Impact factor: 16.971

3.  Cellular locations of Pseudomonas syringae pv. syringae HrcC and HrcJ proteins, required for harpin secretion via the type III pathway.

Authors:  W L Deng; H C Huang
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

4.  Lack of shielding of primer binding site silencer-mediated repression of an internal promoter in a retrovirus vector by the putative insulators scs, BEAD-1, and HS4.

Authors:  C Modin; F S Pedersen; M Duch
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

5.  ATP-binding cassette transport system involved in regulation of morphological differentiation in response to glucose in Streptomyces griseus.

Authors:  Jeong-Woo Seo; Yasuo Ohnishi; Aiko Hirata; Sueharu Horinouchi
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

Review 6.  Gene targeting in the mouse: advances in introduction of transgenes into the genome by homologous recombination.

Authors:  Ravi P Misra; Stephen A Duncan
Journal:  Endocrine       Date:  2002-12       Impact factor: 3.633

7.  High-frequency T-DNA-mediated gene tagging in plants.

Authors:  C Koncz; N Martini; R Mayerhofer; Z Koncz-Kalman; H Körber; G P Redei; J Schell
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

8.  Different relative expression from two murine leukemia virus long terminal repeats in unintegrated transfected DNA and in integrated retroviral vector proviruses.

Authors:  K Paludan; H Y Dai; M Duch; P Jørgensen; N O Kjeldgaard; F S Pedersen
Journal:  J Virol       Date:  1989-12       Impact factor: 5.103

9.  Expression of Erwinia uredovora Phytoene Desaturase in Synechococcus PCC7942 Leading to Resistance against a Bleaching Herbicide.

Authors:  U. Windhovel; B. Geiges; G. Sandmann; P. Boger
Journal:  Plant Physiol       Date:  1994-01       Impact factor: 8.340

10.  Cauliflower mosaic virus promoters direct efficient expression of a bacterial G418 resistance gene in Schizosaccharomyces pombe.

Authors:  H Gmünder; J Kohli
Journal:  Mol Gen Genet       Date:  1989-12
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