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Literature DB >> 24193653

Protoplast-to-plant regeneration in cotton (Gossypium hirsutum L. cv. Coker 312) using feeder layers.

Abstract

We report the regeneration of protoplasts isolated from two embryogenic cell lines of Gossypium hirsutum L. cv. Coker 312 initiated from hypocotylderived callus. Protoplasts plated on cellulose nitrate filters and placed over feeder layers formed embryogenic callus from which plants were regenerated. Plating efficiency up to 12.8% depended upon the cell line. Addition of phytohormones to the protoplast medium had no stimulating effect on plating efficiency. The influence of feeder cells and conditioned medium on plating efficiency was significantly different for the two cell lines.

Entities: Species

Year: 1994 PMID： 24193653 DOI： 10.1007/BF00239894

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

15 in total

1. Transformation of cotton (Gossypium hirsutum L.) via particle bombardment.

Authors: J J Finer; M D McMullen
Journal: Plant Cell Rep Date: 1990-03 Impact factor: 4.570

2. Engineering 2,4-D resistance into cotton.

Authors: C Bayley; N Trolinder; C Ray; M Morgan; J E Quisenberry; D W Ow
Journal: Theor Appl Genet Date: 1992-03 Impact factor: 5.699

3. In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.).

Authors: J M Stewart; C L Hsu
Journal: Planta Date: 1977-01 Impact factor: 4.116

4. Protein staining of ribboned epon sections for light microscopy.

Authors: D B Fisher
Journal: Histochemie Date: 1968

5. Nutrient requirements of suspension cultures of soybean root cells.

Authors: O L Gamborg; R A Miller; K Ojima
Journal: Exp Cell Res Date: 1968-04 Impact factor: 3.905

6. Effect of nurse cultures on the production of macro-calli and fertile plants from maize embryogenic suspension culture protoplasts.

Authors: W L Petersen; S Sulc; C L Armstrong
Journal: Plant Cell Rep Date: 1992-01 Impact factor: 4.570

2. Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion.

Authors: Yuqiang Sun; Xianlong Zhang; Yichun Nie; Xiaoping Guo; Shuangxia Jin; Shaoguang Liang
Journal: Theor Appl Genet Date: 2004-04-28 Impact factor: 5.699

2 in total

Protoplast-to-plant regeneration in cotton (Gossypium hirsutum L. cv. Coker 312) using feeder layers.

1. Transformation of cotton (Gossypium hirsutum L.) via particle bombardment.

2. Engineering 2,4-D resistance into cotton.

3. In-ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.).

4. Protein staining of ribboned epon sections for light microscopy.

5. Nutrient requirements of suspension cultures of soybean root cells.

6. Effect of nurse cultures on the production of macro-calli and fertile plants from maize embryogenic suspension culture protoplasts.

7. Plant regeneration from protoplasts of common buckwheat (fagopyrum esculentum).

8. Insect resistant cotton plants.

9. Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.).

10. Isolation, culture, and cell division in cotyledon protoplasts of cotton (Gossypium hirsutum and G. barbadense).

1. In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsutum L.).

2. Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion.