Literature DB >> 24222414

Liquid chromatography tandem mass spectrometry for measuring ¹³C-labeling in intermediates of the glycolysis and pentose phosphate pathway.

Jean-Christophe Cocuron1, Ana Paula Alonso.   

Abstract

This chapter describes a procedure to analyze (13)C-labeled phosphorylated compounds by liquid chromatography tandem mass spectrometry. Phosphorylated compounds, intermediaries of the glycolysis and pentose phosphate pathway, are separated by anion exchange chromatography and their isotopic labeling is determined by mass spectrometry. A sensitivity in the fmole range is achieved using scheduled multiple reaction monitoring mode.

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Year:  2014        PMID: 24222414     DOI: 10.1007/978-1-62703-688-7_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  11 in total

1.  The gluconate shunt is an alternative route for directing glucose into the pentose phosphate pathway in fission yeast.

Authors:  Mark E Corkins; Stevin Wilson; Jean-Christophe Cocuron; Ana P Alonso; Amanda J Bird
Journal:  J Biol Chem       Date:  2017-06-30       Impact factor: 5.157

2.  A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos.

Authors:  Jean-Christophe Cocuron; Mohamed Koubaa; Rebecca Kimmelfield; Zacchary Ross; Ana Paula Alonso
Journal:  Plant Physiol       Date:  2019-09-17       Impact factor: 8.340

3.  Changes in Whole-Plant Metabolism during the Grain-Filling Stage in Sorghum Grown under Elevated CO2 and Drought.

Authors:  Amanda P De Souza; Jean-Christophe Cocuron; Ana Carolina Garcia; Ana Paula Alonso; Marcos S Buckeridge
Journal:  Plant Physiol       Date:  2015-09-02       Impact factor: 8.340

4.  Source of 12C in Calvin-Benson cycle intermediates and isoprene emitted from plant leaves fed with 13CO2.

Authors:  Thomas D Sharkey; Alyssa L Preiser; Sarathi M Weraduwage; Linus Gog
Journal:  Biochem J       Date:  2020-09-18       Impact factor: 3.857

5.  The Contribution of Metabolomics to Systems Biology: Current Applications Bridging Genotype and Phenotype in Plant Science.

Authors:  Marina C M Martins; Valeria Mafra; Carolina C Monte-Bello; Camila Caldana
Journal:  Adv Exp Med Biol       Date:  2021       Impact factor: 2.622

6.  Metabolite fingerprinting of pennycress (Thlaspi arvense L.) embryos to assess active pathways during oil synthesis.

Authors:  Enkhtuul Tsogtbaatar; Jean-Christophe Cocuron; Marcos Corchado Sonera; Ana Paula Alonso
Journal:  J Exp Bot       Date:  2015-02-22       Impact factor: 6.992

7.  A sweet story: Bean pod mottle virus transmission dynamics by Mexican bean beetles (Epilachna varivestis).

Authors:  Charlotte M Smith; Cassidy R Gedling; Kiana F Wiebe; Bryan J Cassone
Journal:  Genome Biol Evol       Date:  2017-02-15       Impact factor: 3.416

8.  Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis.

Authors:  Enkhtuul Tsogtbaatar; Jean-Christophe Cocuron; Ana Paula Alonso
Journal:  J Exp Bot       Date:  2020-05-30       Impact factor: 6.992

9.  Liquid Chromatography Tandem Mass Spectrometry Quantification of 13C-Labeling in Sugars.

Authors:  Jean-Christophe Cocuron; Zacchary Ross; Ana P Alonso
Journal:  Metabolites       Date:  2020-01-10

10.  Targeted Metabolic Profiles of the Leaves and Xylem Sap of Two Sugarcane Genotypes Infected with the Vascular Bacterial Pathogen Leifsonia xyli subsp. xyli.

Authors:  Fernanda R Castro-Moretti; Jean-Christophe Cocuron; Mariana C Cia; Thais R Cataldi; Carlos A Labate; Ana Paula Alonso; Luis E A Camargo
Journal:  Metabolites       Date:  2021-04-12
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