Literature DB >> 24222035

Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides.

P E Andren1, M R Emmett, R M Caprioli.   

Abstract

The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 μL of a solution containing 320 zeptomolesy/gmL gave an [M + 3H](+3) ion at m/z 558.4 with S/N of > 8∶1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20∶1 for a solution containing 32 attomoles/μL.

Entities:  

Year:  1994        PMID： 24222035     DOI： 10.1016/1044-0305(94)87010-1

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  1 in total

1.  Micro-electrospray mass spectrometry: Ultra-high-sensitivity analysis of peptides and proteins.

Authors:  M R Emmett; R M Caprioli
Journal:  J Am Soc Mass Spectrom       Date:  1994-07       Impact factor: 3.109
  1 in total
  9 in total

1.  Characterizing electrospray ionization using atmospheric pressure ion mobility spectrometry.

Authors:  Xiaoting Tang; James E Bruce; Herbert H Hill
Journal:  Anal Chem       Date:  2006-11-15       Impact factor: 6.986

2.  Effect of decreasing column inner diameter and use of off-line two-dimensional chromatography on metabolite detection in complex mixtures.

Authors:  James L Edwards; Rachel L Edwards; Kendra R Reid; Robert T Kennedy
Journal:  J Chromatogr A       Date:  2007-10-10       Impact factor: 4.759

3.  Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection.

Authors:  N L Kelleher; M W Senko; D P Little; P B O'Connor; F W McLafferty
Journal:  J Am Soc Mass Spectrom       Date:  1995-03       Impact factor: 3.109

4.  Gas phase hydrogen deuterium exchange reactions of a model peptide: FT-ICR and computational analyses of metal induced conformational mutations.

Authors:  T Solouki; R C Fort; A Alomary; A Fattahi
Journal:  J Am Soc Mass Spectrom       Date:  2001-12       Impact factor: 3.109

Review 5.  Recent advances in mass spectrometry analysis of neuropeptides.

Authors:  Ashley Phetsanthad; Nhu Q Vu; Qing Yu; Amanda R Buchberger; Zhengwei Chen; Caitlin Keller; Lingjun Li
Journal:  Mass Spectrom Rev       Date:  2021-09-24       Impact factor: 9.011

6.  Rapid preconcentration for liquid chromatography-mass spectrometry assay of trace level neuropeptides.

Authors:  Ying Zhou; Omar S Mabrouk; Robert T Kennedy
Journal:  J Am Soc Mass Spectrom       Date:  2013-04-17       Impact factor: 3.109

7.  Practical aspects of in vivo detection of neuropeptides by microdialysis coupled off-line to capillary LC with multistage MS.

Authors:  Qiang Li; Jon-Kar Zubieta; Robert T Kennedy
Journal:  Anal Chem       Date:  2009-03-15       Impact factor: 6.986

8.  A simple nanoelectrospray arrangement with controllable flowrate for mass analysis of submicroliter protein samples.

Authors:  B Feng; R D Smith
Journal:  J Am Soc Mass Spectrom       Date:  2000-01       Impact factor: 3.262

9.  Application of micro-electrospray liquid chromatography techniques to FT-ICR MS to enable high-sensitivity biological analysis.

Authors:  M R Emmett; F M White; C L Hendrickson; S D Shi; A G Marshall
Journal:  J Am Soc Mass Spectrom       Date:  1998-04       Impact factor: 3.262
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.