Literature DB >> 24214121

Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection.

N L Kelleher1, M W Senko, D P Little, P B O'Connor, F W McLafferty.   

Abstract

For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5×10(-14) mol (0.5 µL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 µs from a 0.15-µL/min injection of 20-µM ubiquitin consumes 5×10(-18) mol, which produces a spectrum with 23:1 signal-to-noise ratio and τ;3×10(5) resolving power.

Entities:  

Year:  1995        PMID： 24214121     DOI： 10.1016/1044-0305(95)00088-U

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  7 in total

1.  Analysis of antibodies and other large glycoproteins in the mass range of 150,000-200,000 Da by electrospray ionization mass spectrometry.

Authors:  R Feng; Y Konishi
Journal:  Anal Chem       Date:  1992-09-15       Impact factor: 6.986

2.  Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry.

Authors:  D F Hunt; R A Henderson; J Shabanowitz; K Sakaguchi; H Michel; N Sevilir; A L Cox; E Appella; V H Engelhard
Journal:  Science       Date:  1992-03-06       Impact factor: 47.728

3.  Fourier-transform electrospray instrumentation for tandem high-resolution mass spectrometry of large molecules.

Authors:  S C Beu; M W Senko; J P Quinn; F M Wampler; F W McLafferty
Journal:  J Am Soc Mass Spectrom       Date:  1993-07       Impact factor: 3.109

4.  Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides.

Authors:  P E Andren; M R Emmett; R M Caprioli
Journal:  J Am Soc Mass Spectrom       Date:  1994-09       Impact factor: 3.109

5.  Improved detection limits for electrospray ionization on a magnetic sector mass spectrometer by using an array detector.

Authors:  R B Cody; J Tamura; J W Finch; B D Musselman
Journal:  J Am Soc Mass Spectrom       Date:  1994-03       Impact factor: 3.109

6.  Micro-electrospray mass spectrometry: Ultra-high-sensitivity analysis of peptides and proteins.

Authors:  M R Emmett; R M Caprioli
Journal:  J Am Soc Mass Spectrom       Date:  1994-07       Impact factor: 3.109

7.  Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons.

Authors:  M Karas; F Hillenkamp
Journal:  Anal Chem       Date:  1988-10-15       Impact factor: 6.986
  7 in total
  2 in total

1.  The C-score: a Bayesian framework to sharply improve proteoform scoring in high-throughput top down proteomics.

Authors:  Richard D LeDuc; Ryan T Fellers; Bryan P Early; Joseph B Greer; Paul M Thomas; Neil L Kelleher
Journal:  J Proteome Res       Date:  2014-06-12       Impact factor: 4.466

Review 2.  Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.

Authors:  Daniel P Donnelly; Catherine M Rawlins; Caroline J DeHart; Luca Fornelli; Luis F Schachner; Ziqing Lin; Jennifer L Lippens; Krishna C Aluri; Richa Sarin; Bifan Chen; Carter Lantz; Wonhyeuk Jung; Kendall R Johnson; Antonius Koller; Jeremy J Wolff; Iain D G Campuzano; Jared R Auclair; Alexander R Ivanov; Julian P Whitelegge; Ljiljana Paša-Tolić; Julia Chamot-Rooke; Paul O Danis; Lloyd M Smith; Yury O Tsybin; Joseph A Loo; Ying Ge; Neil L Kelleher; Jeffrey N Agar
Journal:  Nat Methods       Date:  2019-06-27       Impact factor: 28.547
  2 in total

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