Literature DB >> 2838499

Immunocytochemical detection of antibodies to Epstein-Barr virus nuclear antigen by a streptavidin-biotin-complex assay.

M Musiani1, M Zerbini, M Plazzi, G Gentilomi, M La Placa.   

Abstract

An immunocytochemical staining for the detection of antibody to Epstein-Barr virus nuclear antigen in cell smears was developed by using a three-dimensional complex of streptavidin and biotin labeled with horseradish peroxidase as the detector system. The presence of antibody against Epstein-Barr virus nuclear antigen was revealed by a dark staining of the nuclei of Raji cells. A significant correlation was found between the titers obtained with our assay and titers obtained by anticomplement immunofluorescence on 110 serum samples. Our assay did not require an Epstein-Barr virus-negative serum source for fresh complement and gave a permanent record of the reaction that could easily be observed under an ordinary light microscope.

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Year:  1988        PMID： 2838499      PMCID： PMC266505          DOI： 10.1128/jcm.26.5.1005-1008.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Indirect alkaline phosphatase immunoenzymatic staining for the detection of antibodies to Epstein-Barr virus-induced virus capsid antigens and early antigens.

Authors:  M Musiani; M Zerbini; G Gentilomi; M La Placa
Journal:  J Clin Microbiol       Date:  1986-02       Impact factor: 5.948

2.  Antibodies to Epstein-Barr virus-associated nuclear antigen in infectious mononucleosis.

Authors:  G Henie; W Henle; C A Horwitz
Journal:  J Infect Dis       Date:  1974-09       Impact factor: 5.226

3.  Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.

Authors:  B M Reedman; G Klein
Journal:  Int J Cancer       Date:  1973-05       Impact factor: 7.396

4.  Typing of herpes simplex virus by capture biotin-streptavidin enzyme-linked immunosorbent assay and comparison with restriction endonuclease analysis and immunofluorescence method using monoclonal antibodies.

Authors:  L S Nerurkar; N R Miller; M Namba; M Monzon; G Brashears; G Scherba; J L Sever
Journal:  J Clin Microbiol       Date:  1987-01       Impact factor: 5.948

5.  Effect of heat shock on Epstein-Barr virus and cytomegalovirus expression.

Authors:  M Zerbini; M Musiani; M La Placa
Journal:  J Gen Virol       Date:  1985-03       Impact factor: 3.891

6.  Rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin-streptavidin enzyme-linked immunosorbent assay.

Authors:  L S Nerurkar; M Namba; G Brashears; A J Jacob; Y J Lee; J L Sever
Journal:  J Clin Microbiol       Date:  1984-07       Impact factor: 5.948

7.  Can Epstein-Barr virus infect and transform all the B-lymphocytes of human cord blood?

Authors:  M Zerbini; I Ernberg
Journal:  J Gen Virol       Date:  1983-03       Impact factor: 3.891

8.  Enzyme immunoassays for the detection of bacterial antigens utilizing biotin-labeled antibody and peroxidase biotin--avidin complex.

Authors:  R H Yolken; F J Leister; L S Whitcomb; M Santosham
Journal:  J Immunol Methods       Date:  1983-02-11       Impact factor: 2.303

9.  Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens.

Authors:  K Adler-Storthz; C Kendall; R C Kennedy; R D Henkel; G R Dreesman
Journal:  J Clin Microbiol       Date:  1983-12       Impact factor: 5.948

10.  Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay.

Authors:  G W Gary; J E Kaplan; S E Stine; L J Anderson
Journal:  J Clin Microbiol       Date:  1985-08       Impact factor: 5.948
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