Literature DB >> 24276446

Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids.

L Menczel1, F Nagy, Z R Kiss, P Maliga.   

Abstract

Protoplasts of Nicotiana tabacum SRI (streptomycin resistant) and of Nicotiana knightiana (streptomycin sensitive) were fused using polyethylene glycol treatment. From three heterokaryons 500 clones were obtained. From the 43 which were further investigated, 6 resistant, 3 sensitive, and 34 chimeric (consisting of resistant and sensitive sectors) calli were found. From eight clones, a total of 39 plants were regenerated and identified as somatic hybrids. Chloroplast type (N. tabacum = NT or N. knightiana = NK) in the plants was determined on the basis of the species specific EcoRI restriction pattern of the chloroplast DNA. Regenerates contained NT (13 plants) or NK (15 plants) plastids but only the plants with the NT chloroplasts were resistant to streptomycin. This finding and our earlier data on uniparental inheritance points to the chloroplasts as the carriers of the streptomycin resistance factor.

Entities:  

Year:  1981        PMID： 24276446     DOI： 10.1007/BF00264975

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  10 in total

1.  Non-Mendelian streptomycin-resistant tobacco mutant with altered chlorplasts and mitochondria.

Authors:  P Maliga; A Sz-Breznovits; F Marton L Joo
Journal:  Nature       Date:  1975-05-29       Impact factor: 49.962

2.  Chloroplast DNA distribution in parasexual hybrids as shown by polypeptide composition of fraction I protein.

Authors:  K Chen; S G Wildman; H H Smith
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

3.  Protein synthesis in isolated spinach chloroplasts: comparison of light-driven and ATP-driven synthesis.

Authors:  W Bottomley; D Spencer; P R Whitfeld
Journal:  Arch Biochem Biophys       Date:  1974-09       Impact factor: 4.013

4.  Can a non-Mendelian mutation affect both chloroplast and mithchondrial ribosomes?

Authors:  J E Boynton; W G Burton; N W Gillham; E H Harris
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

5.  Streptomycin-resistant plants from callus culture of haploid tobacco.

Authors:  P Maliga; A Sz-Breznovits; L Márton
Journal:  Nat New Biol       Date:  1973-07-04

6.  Function of chloroplast DNA. II. Studies on DNA-dependent RNA polymerase activity of tobacco chloroplasts.

Authors:  K K Tewari; S G Wildman
Journal:  Biochim Biophys Acta       Date:  1969-08-20

Review 7.  Inhibitors of ribosome functions.

Authors:  S Pestka
Journal:  Annu Rev Microbiol       Date:  1971       Impact factor: 15.500

8.  An altered chloroplast ribosomal protein in a streptomycin resistant tobacco mutant.

Authors:  N P Yurina; M S Odintsova; P Maliga
Journal:  Theor Appl Genet       Date:  1978-05       Impact factor: 5.699

9.  Chloroplast genes in Chlamydomonas affecting organelle ribosomes. Genetic and biochemical analysis of analysis of antibiotic-resistant mutants at several gene loci.

Authors:  M F Conde; J E Boynton; N W Gillham; E H Harris; C L Tingle; W L Wang
Journal:  Mol Gen Genet       Date:  1975-10-03

10.  Restoration of fertility in cytoplasmic male sterile (CMS) Nicotiana Sylvestris by fusion with X-irradiated N. tabacum protoplasts.

Authors:  D Aviv; E Galun
Journal:  Theor Appl Genet       Date:  1980-05       Impact factor: 5.699
  10 in total
  95 in total

1.  DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins.

Authors:  Je Wook Woo; Jungeun Kim; Soon Il Kwon; Claudia Corvalán; Seung Woo Cho; Hyeran Kim; Sang-Gyu Kim; Sang-Tae Kim; Sunghwa Choe; Jin-Soo Kim
Journal:  Nat Biotechnol       Date:  2015-10-19       Impact factor: 54.908

2.  Differential inhibition of downstream gene expression by the cauliflower mosaic virus 35S RNA leader.

Authors:  J Fütterer; K Gordon; P Pfeiffer; H Sanfaçon; B Pisan; J M Bonneville; T Hohn
Journal:  Virus Genes       Date:  1989-09       Impact factor: 2.332

3.  Genetic transformation of plastids of different Solanaceae species using tobacco cells as organelle hosts.

Authors:  Nikolay Kuchuk; Kateryna Sytnyk; Maxim Vasylenko; Anatolij Shakhovsky; Igor Komarnytsky; Sergei Kushnir; Yuri Gleba
Journal:  Theor Appl Genet       Date:  2006-06-07       Impact factor: 5.699

4.  Plastid transformants of tomato selected using mutations affecting ribosome structure.

Authors:  G D Nugent; M Ten Have; A van der Gulik; P J Dix; B A Uijtewaal; A P Mordhorst
Journal:  Plant Cell Rep       Date:  2005-06-18       Impact factor: 4.570

5.  Production and characterization of intergeneric somatic hybrids of Trachystoma ballii and Brassica juncea.

Authors:  P B Kirti; S B Narasimhulu; S Prakash; V L Chopra
Journal:  Plant Cell Rep       Date:  1992-03       Impact factor: 4.570

6.  Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants.

Authors:  M Nyman; A Wallin
Journal:  Plant Cell Rep       Date:  1992-03       Impact factor: 4.570

7.  Plant regeneration from protoplasts of long-term callus cultures of Actinidia deliciosa var. deliciosa cv. Hayword (Kiwifruit).

Authors:  M M Oliveira; M S Pais
Journal:  Plant Cell Rep       Date:  1991-03       Impact factor: 4.570

8.  Protoplast fusion-derived Ogura male sterile cauliflower with cold tolerance.

Authors:  T W Walters; M A Mutschler; E D Earle
Journal:  Plant Cell Rep       Date:  1992-01       Impact factor: 4.570

9.  Interspecific hybridization between Brassica juncea and B. spinescens through protoplast fusion.

Authors:  P B Kirti; S Prakash; V L Chopra
Journal:  Plant Cell Rep       Date:  1991-03       Impact factor: 4.570

10.  Asymmetric fusion between protoplasts of tomato (Lycopersicon esculentum Mill.) and gamma-irradiated protoplasts of potato (Solanum tuberosum L.): the effects of gamma irradiation.

Authors:  H C Schoenmakers; J J van der Meulen-Muisers; M Koornneef
Journal:  Mol Gen Genet       Date:  1994-02
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