| Literature DB >> 24220706 |
T Pickardt1, M Meixner, V Schade, O Schieder.
Abstract
Shoot tips and epicotyl-segments of Vicia narbonensis were co-cultivated with Agrobacterium tumefaciens strain C58C1 pGV 3850 HPT, carrying a plasmid coding for hygromycin-phosphotransferase. On callus-induction medium containing 60 mg/l hygromycin for selection, approximately 18% of the explants produced hygromycin-resistant callus. After transfer to regeneration-medium these calluses produced hygromycin-resistant and nopaline-positive somatic embryos which could be regenerated to plantlets. The integration of the T-DNA into the plant genome was confirmed by Southern analysis.Entities:
Year: 1991 PMID: 24220706 DOI: 10.1007/BF00232326
Source DB: PubMed Journal: Plant Cell Rep ISSN: 0721-7714 Impact factor: 4.570