Literature DB >> 24220903

Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens - mediated gene transfer.

J Puonti-Kaerlas1, T Eriksson, P Engström.   

Abstract

A transformation system that allows regeneration of transgenic pea plants from calli selected for antibiotic resistance was developed. Explants from axenic shoot cultures and seedling epicotyls were cocultivated with nononcogenic Agrobacterium tumefaciens strains, and transformed callus could be selected on callus-inducing media containing either 15 mg/l hygromycin or 75 mg/l kanamycin. After several passages on regeneration medium, shoot organogenesis could be reproducibly induced on hygromycin-resistant calli, but not on the calli selected for kanamycin resistance. Regenerated shoots could subsequently be rooted and transferred into the greenhouse. In addition, the effects of different callus-inducing and growth media on organogenesis were investigated. The transformation of the calli and regenerated plants was confirmed by DNA analysis.

Entities:  

Year:  1990        PMID： 24220903     DOI： 10.1007/BF00224394

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  18 in total

1.  Plant regeneration from pea protoplasts via somatic embyogenesis.

Authors:  R Lehminger-Mertens; H J Jacobsen
Journal:  Plant Cell Rep       Date:  1989-10       Impact factor: 4.570

Review 2.  Foreign genes in plants: transfer, structure, expression, and applications.

Authors:  K Weising; J Schell; G Kahl
Journal:  Annu Rev Genet       Date:  1988       Impact factor: 16.830

3.  The functional organization of the nopaline A. tumefaciens plasmid pTiC58.

Authors:  M Holsters; B Silva; F Van Vliet; C Genetello; M De Block; P Dhaese; A Depicker; D Inzé; G Engler; R Villarroel
Journal:  Plasmid       Date:  1980-03       Impact factor: 3.466

4.  Genetically transformed maize plants from protoplasts.

Authors:  C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal:  Science       Date:  1988-04-08       Impact factor: 47.728

5.  Selection-expression plasmid vectors for use in genetic transformation of higher plants.

Authors:  J Velten; J Schell
Journal:  Nucleic Acids Res       Date:  1985-10-11       Impact factor: 16.971

6.  Specificity of strain and genotype in the susceptibility of pea to Agrobacterium tumefaciens.

Authors:  S L Hobbs; J A Jackson; J D Mahon
Journal:  Plant Cell Rep       Date:  1989-05       Impact factor: 4.570

7.  Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants.

Authors:  R Deblaere; B Bytebier; H De Greve; F Deboeck; J Schell; M Van Montagu; J Leemans
Journal:  Nucleic Acids Res       Date:  1985-07-11       Impact factor: 16.971

8.  Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens.

Authors:  S McCormick; J Niedermeyer; J Fry; A Barnason; R Horsch; R Fraley
Journal:  Plant Cell Rep       Date:  1986-04       Impact factor: 4.570

9.  Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens.

Authors:  E Van Haute; H Joos; M Maes; G Warren; M Van Montagu; J Schell
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

10.  Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity.

Authors:  P Zambryski; H Joos; C Genetello; J Leemans; M V Montagu; J Schell
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
View more
  22 in total

Review 1.  Feasibility of Pisum sativum as an expression system for pharmaceuticals.

Authors:  Heike Mikschofsky; Inge Broer
Journal:  Transgenic Res       Date:  2011-11-06       Impact factor: 2.788

2.  A rapid and efficient regeneration system for pea (Pisum sativum), suitable for transformation.

Authors:  B Nauerby; M Madsen; J Christiansen; R Wyndaele
Journal:  Plant Cell Rep       Date:  1991-04       Impact factor: 4.570

3.  Inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plants.

Authors:  J Puonti-Kaerlas; T Eriksson; P Engström
Journal:  Theor Appl Genet       Date:  1992-07       Impact factor: 5.699

4.  Agrobacterium tumefaciens-mediated beta-glucuronidase (GUS) gene expression in lentil (Lens culinaris Medik.) tissues.

Authors:  T D Warkentin; A McHughen
Journal:  Plant Cell Rep       Date:  1992-06       Impact factor: 4.570

5.  Efficient transgenic plant regeneration throughAgrobacterium-mediated transformation of Chickpea (Cicer arietinum L.).

Authors:  S Kar; T M Johnson; P Nayak; S K Sen
Journal:  Plant Cell Rep       Date:  1996-11       Impact factor: 4.570

6.  Genetic transformation of cotyledon explants of cowpea (Vigna unguiculata L. Walp) using Agrobacterium tumefaciens.

Authors:  B Muthukumar; M Mariamma; K Veluthambi; A Gnanam
Journal:  Plant Cell Rep       Date:  1996-09       Impact factor: 4.570

7.  A simple system for pea transformation.

Authors:  S J Bean; P S Gooding; P M Mullincaux; D R Davies
Journal:  Plant Cell Rep       Date:  1997-05       Impact factor: 4.570

8.  Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera).

Authors:  A Richter; H-J Jacobsen; A de Kathen; G de Lorenzo; K Briviba; R Hain; G Ramsay; H Kiesecker
Journal:  Plant Cell Rep       Date:  2006-06-27       Impact factor: 4.570

9.  High frequency adventitious shoot regeneration from immature cotyledons of pea (Pisum sativum L.).

Authors:  S Ozcan; M Barghchi; S Firek; J Draper
Journal:  Plant Cell Rep       Date:  1992-02       Impact factor: 4.570

10.  Agrobacterium tumefaciens mediated gene transfer in peanut (Arachis hypogaea L.).

Authors:  S Eapen; L George
Journal:  Plant Cell Rep       Date:  1994-07       Impact factor: 4.570
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.