Literature DB >> 24203131

Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants.

G A Moore1, C C Jacono, J L Neidigh, S D Lawrence, K Cline.   

Abstract

A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker ß-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (≤ 5%) of the shoots regenerated in the presence of 100 μg/ml kanamycin were GUS(+). Polymerase chain reaction (PCR) analysis confirmed that GUS(+) shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.

Entities:  

Year:  1992        PMID： 24203131     DOI： 10.1007/BF00235073

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  7 in total

1.  Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens.

Authors:  R K Jayaswal; K Veluthambi; S B Gelvin; J L Slightom
Journal:  J Bacteriol       Date:  1987-11       Impact factor: 3.490

2.  Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues.

Authors:  S O Rogers; A J Bendich
Journal:  Plant Mol Biol       Date:  1985-03       Impact factor: 4.076

3.  Transformed callus does not necessarily regenerate transformed shoots.

Authors:  M C Jordan; A McHughen
Journal:  Plant Cell Rep       Date:  1988-06       Impact factor: 4.570

4.  Genomic sequencing.

Authors:  G M Church; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

5.  The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.

Authors:  E E Hood; G L Helmer; R T Fraley; M D Chilton
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

6.  Physical and functional map of supervirulent Agrobacterium tumefaciens tumor-inducing plasmid pTiBo542.

Authors:  T Komari; W Halperin; E W Nester
Journal:  J Bacteriol       Date:  1986-04       Impact factor: 3.490

7.  New cloning vehicles for transformation of higher plants.

Authors:  G An; B D Watson; S Stachel; M P Gordon; E W Nester
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598
  7 in total
  23 in total

1.  An embryogenic suspension cell culture system for Agrobacterium-mediated transformation of citrus.

Authors:  M Dutt; J W Grosser
Journal:  Plant Cell Rep       Date:  2010-08-14       Impact factor: 4.570

2.  Biolistic transformation of Carrizo citrange (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.).

Authors:  Hao Wu; Yosvanis Acanda; Hongge Jia; Nian Wang; Janice Zale
Journal:  Plant Cell Rep       Date:  2016-06-08       Impact factor: 4.570

3.  Citrus biotechnology: Achievements, limitations and future directions.

Authors:  Sandeepa Singh; Manchikatla V Rajam
Journal:  Physiol Mol Biol Plants       Date:  2009-05-14

4.  Establishment of an in vitro regeneration system and genetic transformation of the Tunisian 'Maltese half-blood' (Citrus sinensis): an agro-economically important variety.

Authors:  Rahma Jardak; Hatem Boubakri; Hassene Zemni; Samia Gandoura; Samiha Mejri; Ahmed Mliki; Abdelwahed Ghorbel
Journal:  3 Biotech       Date:  2020-02-06       Impact factor: 2.406

5.  Genetic transformation and regeneration of rubber tree (Hevea brasiliensis Muell. Arg) transgenic plants with a constitutive version of an anti-oxidative stress superoxide dismutase gene.

Authors:  R Jayashree; K Rekha; P Venkatachalam; S L Uratsu; A M Dandekar; P Kumari Jayasree; R G Kala; P Priya; S Sushma Kumari; S Sobha; M P Ashokan; M R Sethuraj; A Thulaseedharan
Journal:  Plant Cell Rep       Date:  2003-07-09       Impact factor: 4.570

6.  Introduction of a citrus blight-associated gene into Carrizo citrange [Citrus sinensis (L.) Osbc. x Poncirus trifoliata (L.) Raf.] by Agrobacterium-mediated transformation.

Authors:  M Kayim; T L Ceccardi; M J G Berretta; G A Barthe; K S Derrick
Journal:  Plant Cell Rep       Date:  2004-07-10       Impact factor: 4.570

7.  High-efficiency Agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration.

Authors:  Maria Luiza Peixoto de Oliveira; Vicente J Febres; Marcio Gilberto Cardoso Costa; Gloria A Moore; Wagner Campos Otoni
Journal:  Plant Cell Rep       Date:  2008-12-02       Impact factor: 4.570

8.  Early events in Agrobacterium-mediated genetic transformation of citrus explants.

Authors:  Leandro Peña; Rosa M Pérez; Magdalena Cervera; José A Juárez; Luis Navarro
Journal:  Ann Bot       Date:  2004-05-14       Impact factor: 4.357

9.  A simple and efficient gene transfer system of trifoliate orange (Poncirus trifoliata Raf.).

Authors:  J Kaneyoshi Hiramatsu; S Kobayashi; Y Nakamura; N Shigemoto; Y Doi
Journal:  Plant Cell Rep       Date:  1994-07       Impact factor: 4.570

10.  Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants.

Authors:  L Peña; M Cervera; J Juárez; A Navarro; J A Pina; N Durán-Vila; L Navarro
Journal:  Plant Cell Rep       Date:  1995-07       Impact factor: 4.570
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.