Literature DB >> 24194307

Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants.

L Peña1, M Cervera, J Juárez, A Navarro, J A Pina, N Durán-Vila, L Navarro.   

Abstract

Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and ß-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.

Entities:  

Year:  1995        PMID： 24194307     DOI： 10.1007/BF00232724

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  5 in total

1.  Genetic transformation of apple (Malus pumila Mill.) using a disarmed Ti-binary vector.

Authors:  D J James; A J Passey; D J Barbara; M Bevan
Journal:  Plant Cell Rep       Date:  1989-03       Impact factor: 4.570

2.  Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants.

Authors:  G A Moore; C C Jacono; J L Neidigh; S D Lawrence; K Cline
Journal:  Plant Cell Rep       Date:  1992-06       Impact factor: 4.570

3.  The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.

Authors:  E E Hood; G L Helmer; R T Fraley; M D Chilton
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

4.  Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation.

Authors:  G Vancanneyt; R Schmidt; A O'Connor-Sanchez; L Willmitzer; M Rocha-Sosa
Journal:  Mol Gen Genet       Date:  1990-01

5.  GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors:  R A Jefferson; T A Kavanagh; M W Bevan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598
  5 in total
  16 in total

1.  Evaluation of selection strategies alternative to nptII in genetic transformation of citrus.

Authors:  Alida Ballester; Magdalena Cervera; Leandro Peña
Journal:  Plant Cell Rep       Date:  2008-03-04       Impact factor: 4.570

2.  Inducible expression of Bs2 R gene from Capsicum chacoense in sweet orange (Citrus sinensis L. Osbeck) confers enhanced resistance to citrus canker disease.

Authors:  Lorena Noelia Sendín; Ingrid Georgina Orce; Rocío Liliana Gómez; Ramón Enrique; Carlos Froilán Grellet Bournonville; Aldo Sergio Noguera; Adrián Alberto Vojnov; María Rosa Marano; Atilio Pedro Castagnaro; María Paula Filippone
Journal:  Plant Mol Biol       Date:  2017-02-02       Impact factor: 4.076

3.  Citrus biotechnology: Achievements, limitations and future directions.

Authors:  Sandeepa Singh; Manchikatla V Rajam
Journal:  Physiol Mol Biol Plants       Date:  2009-05-14

4.  Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination.

Authors:  Alida Ballester; Magdalena Cervera; Leandro Peña
Journal:  Plant Cell Rep       Date:  2006-08-23       Impact factor: 4.570

5.  Transformation of carotenoid biosynthetic genes using a micro-cross section method in kiwifruit (Actinidia deliciosa cv. Hayward).

Authors:  Misun Kim; Seong-Cheol Kim; Kwan Jeong Song; Ho Bang Kim; In-Jung Kim; Eun-Young Song; Seung-Jong Chun
Journal:  Plant Cell Rep       Date:  2010-09-15       Impact factor: 4.570

6.  High-efficiency Agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration.

Authors:  Maria Luiza Peixoto de Oliveira; Vicente J Febres; Marcio Gilberto Cardoso Costa; Gloria A Moore; Wagner Campos Otoni
Journal:  Plant Cell Rep       Date:  2008-12-02       Impact factor: 4.570

7.  The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck).

Authors:  R L Boscariol; W A B Almeida; M T V C Derbyshire; F A A Mourão Filho; B M J Mendes
Journal:  Plant Cell Rep       Date:  2003-07-19       Impact factor: 4.570

8.  Early antibiotic selection and efficient rooting and acclimatization improve the production of transgenic plum plants (Prunus domestica L.).

Authors:  I M Gonzalez Padilla; K Webb; R Scorza
Journal:  Plant Cell Rep       Date:  2003-06-24       Impact factor: 4.570

9.  An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush.

Authors:  Balwinder Singh; Gurdeep Singh Virk; Avinash Kaur Nagpal
Journal:  Physiol Mol Biol Plants       Date:  2011-04-20

10.  Development of an efficient transformation method by Agrobacterium tumefaciens and high throughput spray assay to identify transgenic plants for woodland strawberry (Fragaria vesca) using NPTII selection.

Authors:  Christopher J Pantazis; Sarah Fisk; Kerri Mills; Barry S Flinn; Vladimir Shulaev; Richard E Veilleux; Yinghui Dan
Journal:  Plant Cell Rep       Date:  2012-11-16       Impact factor: 4.570
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