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Literature DB >> 24196586

Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry.

Amanda Jane Hughes¹, Matthew R Tibby, Drew T Wagner, Johnathan N Brantley, Adrian T Keatinge-Clay.

Abstract

A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative.

Entities: Chemical Disease Gene Species

Mesh：

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Year: 2013 PMID： 24196586 PMCID： PMC3999232 DOI： 10.1039/c3cc47513a

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

15 in total

Review 1. The structures of type I polyketide synthases.

Authors: Adrian T Keatinge-Clay
Journal: Nat Prod Rep Date: 2012-08-03 Impact factor: 13.423

2. A mechanism-based fluorescence transfer assay for examining ketosynthase selectivity.

Authors: Gitanjeli Prasad; Lawrence S Borketey; Tsung-Yi Lin; Nathan A Schnarr
Journal: Org Biomol Chem Date: 2012-07-18 Impact factor: 3.876

3. Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket.

Authors: Colin J B Harvey; Joseph D Puglisi; Vijay S Pande; David E Cane; Chaitan Khosla
Journal: J Am Chem Soc Date: 2012-07-11 Impact factor: 15.419

4. Employing modular polyketide synthase ketoreductases as biocatalysts in the preparative chemoenzymatic syntheses of diketide chiral building blocks.

Authors: Shawn K Piasecki; Clint A Taylor; Joshua F Detelich; June Liu; Jianting Zheng; Arkady Komsoukaniants; Dionicio R Siegel; Adrian T Keatinge-Clay
Journal: Chem Biol Date: 2011-10-28

5. Enzymatic extender unit generation for in vitro polyketide synthase reactions: structural and functional showcasing of Streptomyces coelicolor MatB.

Authors: Amanda J Hughes; Adrian Keatinge-Clay
Journal: Chem Biol Date: 2011-02-25

Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry.

Review 1. The structures of type I polyketide synthases.

2. A mechanism-based fluorescence transfer assay for examining ketosynthase selectivity.

3. Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket.

4. Employing modular polyketide synthase ketoreductases as biocatalysts in the preparative chemoenzymatic syntheses of diketide chiral building blocks.

5. Enzymatic extender unit generation for in vitro polyketide synthase reactions: structural and functional showcasing of Streptomyces coelicolor MatB.

6. The biochemical basis for stereochemical control in polyketide biosynthesis.

Review 7. The type I fatty acid and polyketide synthases: a tale of two megasynthases.

8. Biocatalytic synthesis of pikromycin, methymycin, neomethymycin, novamethymycin, and ketomethymycin.

9. Preparative, in vitro biocatalysis of triketide lactone chiral building blocks.

10. In vivo imaging of membrane-associated glycans in developing zebrafish.

Review 1. Recent Advances in Understanding and Engineering Polyketide Synthesis.

Review 2. Acyltransferases as Tools for Polyketide Synthase Engineering.

3. Engineered Biosynthesis of Alkyne-Tagged Polyketides by Type I PKSs.