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Literature DB >> 24187681

Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores.

Abstract

We have developed new protein-based tags for use in imaging of RNAs in living cells. These tags are based on the MS2 phage coat protein fused to either E. coli dihydrofolate reductase or the SNAP tag. Labeled RNAs can be visualized with fluorophores spanning the visible spectra range.

Entities: Chemical Gene Species

Mesh：

Substances：
Fluorescent Dyes
RNA

Year: 2013 PMID： 24187681 PMCID： PMC3873762 DOI： 10.1039/c3an01550e

Source DB: PubMed Journal: Analyst ISSN： 0003-2654 Impact factor: 4.616

14 in total

1. A general method for the covalent labeling of fusion proteins with small molecules in vivo.

Authors: Antje Keppler; Susanne Gendreizig; Thomas Gronemeyer; Horst Pick; Horst Vogel; Kai Johnsson
Journal: Nat Biotechnol Date: 2002-12-09 Impact factor: 54.908

2. Real-time observation of transcription initiation and elongation on an endogenous yeast gene.

Authors: Daniel R Larson; Daniel Zenklusen; Bin Wu; Jeffrey A Chao; Robert H Singer
Journal: Science Date: 2011-04-22 Impact factor: 47.728

Review 3. Searching for the protein targets of bioactive molecules.

Authors: Christopher Chidley; Hirohito Haruki; Miriam Grønlund Pedersen; Cindy Fellay; Simone Moser; Kai Johnsson
Journal: Chimia (Aarau) Date: 2011 Impact factor: 1.509

4. In vivo protein labeling with trimethoprim conjugates: a flexible chemical tag.

Authors: Lawrence W Miller; Yunfei Cai; Michael P Sheetz; Virginia W Cornish
Journal: Nat Methods Date: 2005-04 Impact factor: 28.547

Review 5. The fluorescent toolbox for assessing protein location and function.

Authors: Ben N G Giepmans; Stephen R Adams; Mark H Ellisman; Roger Y Tsien
Journal: Science Date: 2006-04-14 Impact factor: 47.728

6. Subunit fusion confers tolerance to peptide insertions in a virus coat protein.

Authors: D S Peabody
Journal: Arch Biochem Biophys Date: 1997-11-01 Impact factor: 4.013

7. Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells.

Authors: Bin Wu; Jeffrey A Chao; Robert H Singer
Journal: Biophys J Date: 2012-06-19 Impact factor: 4.033

8. RNA mimics of green fluorescent protein.

Authors: Jeremy S Paige; Karen Y Wu; Samie R Jaffrey
Journal: Science Date: 2011-07-29 Impact factor: 47.728

9. Localization of ASH1 mRNA particles in living yeast.

Authors: E Bertrand; P Chartrand; M Schaefer; S M Shenoy; R H Singer; R M Long
Journal: Mol Cell Date: 1998-10 Impact factor: 17.970

10. Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging.

Authors: Xiaoli Sun; Aihua Zhang; Brenda Baker; Luo Sun; Angela Howard; John Buswell; Damien Maurel; Anastasiya Masharina; Kai Johnsson; Christopher J Noren; Ming-Qun Xu; Ivan R Corrêa
Journal: Chembiochem Date: 2011-07-26 Impact factor: 3.164

7 in total

Review 1. RNA Localization in Bacteria.

Authors: Jingyi Fei; Cynthia M Sharma
Journal: Microbiol Spectr Date: 2018-09

Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores.

1. A general method for the covalent labeling of fusion proteins with small molecules in vivo.

2. Real-time observation of transcription initiation and elongation on an endogenous yeast gene.

Review 3. Searching for the protein targets of bioactive molecules.

4. In vivo protein labeling with trimethoprim conjugates: a flexible chemical tag.

Review 5. The fluorescent toolbox for assessing protein location and function.

6. Subunit fusion confers tolerance to peptide insertions in a virus coat protein.

7. Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells.

8. RNA mimics of green fluorescent protein.

9. Localization of ASH1 mRNA particles in living yeast.

10. Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging.

Review 1. RNA Localization in Bacteria.

Review 2. Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes.

Review 3. In the right place at the right time: visualizing and understanding mRNA localization.

4. In vivo single-RNA tracking shows that most tRNA diffuses freely in live bacteria.

5. Evolution and characterization of a benzylguanine-binding RNA aptamer.

Review 6. Illuminating Messengers: An Update and Outlook on RNA Visualization in Bacteria.

7. Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells.