Literature DB >> 24185445

Green fluorescent protein: an in vivo reporter of plant gene expression.

R P Niedz1, M R Sussman, J S Satterlee.   

Abstract

Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.

Entities:  

Year:  1995        PMID： 24185445     DOI： 10.1007/BF00234043

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  12 in total

1.  New vectors for high level expression of recombinant proteins in bacteria.

Authors:  D J Hakes; J E Dixon
Journal:  Anal Biochem       Date:  1992-05-01       Impact factor: 3.365

2.  Transient gene expression in tobacco protoplasts: II. Comparison of the reporter gene systems for CAT, NPT II, and GUS.

Authors:  R Töpfer; M Pröls; J Schell; H H Steinbiß
Journal:  Plant Cell Rep       Date:  1988-06       Impact factor: 4.570

3.  Effects of electroporation pulse wave on the incorporation of viral RNA into tobacco protoplasts.

Authors:  J A Saunders; C R Smith; J M Kaper
Journal:  Biotechniques       Date:  1989 Nov-Dec       Impact factor: 1.993

4.  The isolation, culture and regeneration of Petunia leaf protoplasts.

Authors:  E M Frearson; J B Power; E C Cocking
Journal:  Dev Biol       Date:  1973-07       Impact factor: 3.582

5.  Reversible denaturation of Aequorea green-fluorescent protein: physical separation and characterization of the renatured protein.

Authors:  W W Ward; S H Bokman
Journal:  Biochemistry       Date:  1982-09-14       Impact factor: 3.162

6.  Green fluorescent protein as a marker for gene expression.

Authors:  M Chalfie; Y Tu; G Euskirchen; W W Ward; D C Prasher
Journal:  Science       Date:  1994-02-11       Impact factor: 47.728

7.  Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts.

Authors:  B Damm; R Schmidt; L Willmitzer
Journal:  Mol Gen Genet       Date:  1989-05

8.  Primary structure of the Aequorea victoria green-fluorescent protein.

Authors:  D C Prasher; V K Eckenrode; W W Ward; F G Prendergast; M J Cormier
Journal:  Gene       Date:  1992-02-15       Impact factor: 3.688

9.  Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein.

Authors:  C W Cody; D C Prasher; W M Westler; F G Prendergast; W W Ward
Journal:  Biochemistry       Date:  1993-02-09       Impact factor: 3.162

10.  Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis.

Authors:  S Wang; T Hazelrigg
Journal:  Nature       Date:  1994-06-02       Impact factor: 49.962
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  15 in total

1.  Characterization of gene expression during potato tuber development in individuals and populations using the luciferase reporter system.

Authors:  John Verhees; Alexander R van der Krol; Dick Vreugdenhil; Linus H W van der Plas
Journal:  Plant Mol Biol       Date:  2002-11       Impact factor: 4.076

2.  Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants.

Authors:  H A Richards; M D Halfhill; R J Millwood; C N Stewart
Journal:  Plant Cell Rep       Date:  2003-07-04       Impact factor: 4.570

3.  Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice.

Authors:  Jianzhong Lin; Bo Zhou; Yuanzhu Yang; Jin Mei; Xiaoying Zhao; Xinhong Guo; Xingqun Huang; Dongying Tang; Xuanming Liu
Journal:  Plant Cell Rep       Date:  2009-05-20       Impact factor: 4.570

4.  Fusions between green fluorescent protein and beta-glucuronidase as sensitive and vital bifunctional reporters in plants.

Authors:  N E Quaedvlieg; H R Schlaman; P C Admiraal; S E Wijting; J Stougaard; H P Spaink
Journal:  Plant Mol Biol       Date:  1998-11       Impact factor: 4.076

5.  Investigation of Agrobacterium-mediated transformation of apple using green fluorescent protein: high transient expression and low stable transformation suggest that factors other than T-DNA transfer are rate-limiting.

Authors:  S N Maximova; A M Dandekar; M J Guiltinan
Journal:  Plant Mol Biol       Date:  1998-06       Impact factor: 4.076

6.  Enhanced expression in tobacco of the gene encoding green fluorescent protein by modification of its codon usage.

Authors:  G J Rouwendal; O Mendes; E J Wolbert; A Douwe de Boer
Journal:  Plant Mol Biol       Date:  1997-04       Impact factor: 4.076

7.  Expression of the green fluorescent protein gene in conifer tissues.

Authors:  Lining Tian; Armand Séguin; Pierre J Charest
Journal:  Plant Cell Rep       Date:  1997-02       Impact factor: 4.570

8.  Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.).

Authors:  Hanyan Gu; Yuying Jia; Xiansheng Wang; Quanjia Chen; Shubing Shi; Lin Ma; Jusong Zhang; Hua Zhang; Hao Ma
Journal:  Mol Biol Rep       Date:  2011-07-01       Impact factor: 2.316

9.  Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants.

Authors:  S J Davis; R D Vierstra
Journal:  Plant Mol Biol       Date:  1998-03       Impact factor: 4.076

10.  Fusions between green fluorescent protein and beta-glucuronidase as sensitive and vital bifunctional reporters in plants.

Authors:  N E Quaedvlieg; H R Schlaman; P C Admiraal; S E Wijting; J Stougaard; H P Spaink
Journal:  Plant Mol Biol       Date:  1998-07       Impact factor: 4.076
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