| Literature DB >> 2418443 |
P T Ma, T Yamamoto, J L Goldstein, M S Brown.
Abstract
Pharmacologic doses of 17 alpha-ethinyl estradiol are known to increase the number of low density lipoprotein (LDL) receptors in livers of rats, thereby producing a profound fall in plasma cholesterol levels. We now report that ethinyl estradiol exerts the same effect in livers of male and female rabbits and that the increase in receptor number is correlated with a 6- to 8-fold increase in the levels of receptor mRNA. Receptor protein was measured by ligand blotting, and mRNA levels were measured by a quantitative solution hybridization/S1 nuclease protection assay using uniformly 32P-labeled single-stranded cDNA probes. These experiments demonstrate that pharmacologic induction of the mRNA for the LDL receptor in liver can lead to increased LDL receptor levels and a fall in plasma cholesterol in experimental animals.Entities:
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Year: 1986 PMID： 2418443 PMCID： PMC322951 DOI： 10.1073/pnas.83.3.792
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205