Literature DB >> 2418349

Effects of DNA methylation on specific transcription by RNA polymerase II in vitro.

P L Molloy.   

Abstract

In vitro transcription in a HeLa cell lysate by RNA polymerase II directed by a chicken feather keratin gene promotor has been studied using unmethylated template DNA and DNA methylated in vitro by HpaII methylase. The efficiency of specific gene transcription from methylated DNA was dependent on topology of the input DNA, the most significant effect being complete inhibition of transcription from one template which contained three methylation sites, one just 5' and two greater than 500 bases 3' to the site of transcription initiation. The inhibition of transcription depends on a factor(s) which is variably present in lysate preparations and is labile on storage at -70 degrees.

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Year:  1986        PMID： 2418349     DOI： 10.1007/bf00417589

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  19 in total

1.  The somatic replication of DNA methylation.

Authors:  M Wigler; D Levy; M Perucho
Journal:  Cell       Date:  1981-04       Impact factor: 41.582

2.  In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells.

Authors:  R Stein; A Razin; H Cedar
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

3.  DNA methylation and the regulation of globin gene expression.

Authors:  M Busslinger; J Hurst; R A Flavell
Journal:  Cell       Date:  1983-08       Impact factor: 41.582

4.  Clonal inheritance of the pattern of DNA methylation in mouse cells.

Authors:  R Stein; Y Gruenbaum; Y Pollack; A Razin; H Cedar
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

5.  Expression of a cloned adenovirus gene is inhibited by in vitro methylation.

Authors:  L Vardimon; A Kressmann; H Cedar; M Maechler; W Doerfler
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

6.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

7.  Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.

Authors:  L Katz; D T Kingsbury; D R Helinski
Journal:  J Bacteriol       Date:  1973-05       Impact factor: 3.490

Review 8.  Eukaryotic DNA methylation.

Authors:  D N Cooper
Journal:  Hum Genet       Date:  1983       Impact factor: 4.132

9.  In vitro methylation of specific regions of the cloned Moloney sarcoma virus genome inhibits its transforming activity.

Authors:  M L McGeady; C Jhappan; R Ascione; G F Vande Woude
Journal:  Mol Cell Biol       Date:  1983-03       Impact factor: 4.272

10.  Transcription of methylated eukaryotic viral genes in a soluble in vitro system.

Authors:  R Jove; D E Sperber; J L Manley
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971
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  2 in total

1.  Dynamics of MBD2 deposition across methylated DNA regions during malignant transformation of human mammary epithelial cells.

Authors:  Guillaume Devailly; Mélodie Grandin; Laury Perriaud; Pauline Mathot; Jean-Guy Delcros; Yannick Bidet; Anne-Pierre Morel; Jean-Yves Bignon; Alain Puisieux; Patrick Mehlen; Robert Dante
Journal:  Nucleic Acids Res       Date:  2015-05-24       Impact factor: 16.971

2.  Excess Light Priming in Arabidopsis thaliana Genotypes with Altered DNA Methylomes.

Authors:  Diep R Ganguly; Bethany A B Stone; Andrew F Bowerman; Steven R Eichten; Barry J Pogson
Journal:  G3 (Bethesda)       Date:  2019-11-05       Impact factor: 3.154
  2 in total

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