| Literature DB >> 24161985 |
Tilmann Bürckstümmer1, Carina Banning, Philipp Hainzl, Richard Schobesberger, Claudia Kerzendorfer, Florian M Pauler, Doris Chen, Nicole Them, Fiorella Schischlik, Manuele Rebsamen, Michal Smida, Ferran Fece de la Cruz, Ana Lapao, Melissa Liszt, Benjamin Eizinger, Philipp M Guenzl, Vincent A Blomen, Tomasz Konopka, Bianca Gapp, Katja Parapatics, Barbara Maier, Johannes Stöckl, Wolfgang Fischl, Sejla Salic, M Rita Taba Casari, Sylvia Knapp, Keiryn L Bennett, Christoph Bock, Jacques Colinge, Robert Kralovics, Gustav Ammerer, Georg Casari, Thijn R Brummelkamp, Giulio Superti-Furga, Sebastian M B Nijman.
Abstract
Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-α, TGF-β, IFN-γ and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines.Entities:
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Year: 2013 PMID： 24161985 PMCID： PMC6342250 DOI： 10.1038/nmeth.2609
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547