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Literature DB >> 24126761

hPrimpol1/CCDC111 is a human DNA primase-polymerase required for the maintenance of genome integrity.

Li Wan¹, Jiangman Lou, Yisui Xia, Bei Su, Ting Liu, Jiamin Cui, Yingying Sun, Huiqiang Lou, Jun Huang.

Abstract

Prim-pol is a recently identified DNA primase-polymerase belonging to the archaeao-eukaryotic primase (AEP) superfamily. Here, we characterize a previously unrecognized prim-pol in human cells, which we designate hPrimpol1 (human primase-polymerase 1). hPrimpol1 possesses primase and DNA polymerase activities in vitro, interacts directly with RPA1 and is recruited to sites of DNA damage and stalled replication forks in an RPA1-dependent manner. Cells depleted of hPrimpol1 display increased spontaneous DNA damage and defects in the restart of stalled replication forks. Both RPA1 binding and the primase activity of hPrimpol1 are required for its cellular function during DNA replication. Our results indicate that hPrimpol1 is a novel factor involved in the response to DNA replication stress.

Entities: Gene Species

Mesh：

Substances：

Year: 2013 PMID： 24126761 PMCID： PMC3981091 DOI： 10.1038/embor.2013.159

Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807

21 in total

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2. Modulation of hyperthermophilic DNA polymerase activity by archaeal chromatin proteins.

Authors: Huiqiang Lou; Zhenhong Duan; Xiaofeng Huo; Li Huang
Journal: J Biol Chem Date: 2003-10-16 Impact factor: 5.157

3. A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activity.

Authors: Georg Lipps; Susanne Röther; Christina Hart; Gerhard Krauss
Journal: EMBO J Date: 2003-05-15 Impact factor: 11.598

4. Structure of a bifunctional DNA primase-polymerase.

Authors: Georg Lipps; Andreas O Weinzierl; Gudrun von Scheven; Claudia Buchen; Patrick Cramer
Journal: Nat Struct Mol Biol Date: 2004-01-18 Impact factor: 15.369

5. ATR phosphorylates SMARCAL1 to prevent replication fork collapse.

Authors: Frank B Couch; Carol E Bansbach; Robert Driscoll; Jessica W Luzwick; Gloria G Glick; Rémy Bétous; Clinton M Carroll; Sung Yun Jung; Jun Qin; Karlene A Cimprich; David Cortez
Journal: Genes Dev Date: 2013-07-15 Impact factor: 11.361

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Journal: Annu Rev Biochem Date: 1997 Impact factor: 23.643

7. Independent and coordinated functions of replication protein A tandem high affinity single-stranded DNA binding domains.

Authors: Alphonse I Arunkumar; Melissa E Stauffer; Elena Bochkareva; Alexey Bochkarev; Walter J Chazin
Journal: J Biol Chem Date: 2003-07-24 Impact factor: 5.157

8. Key role of template sequence for primer synthesis by the herpes simplex virus 1 helicase-primase.

Authors: Kathryn A Ramirez-Aguilar; Nisha A Low-Nam; Robert D Kuchta
Journal: Biochemistry Date: 2002-12-10 Impact factor: 3.162

9. UV stalled replication forks restart by re-priming in human fibroblasts.

Authors: Ingegerd Elvers; Fredrik Johansson; Petra Groth; Klaus Erixon; Thomas Helleday
Journal: Nucleic Acids Res Date: 2011-06-06 Impact factor: 16.971

10. Origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural insights and new members.

Authors: Lakshminarayan M Iyer; Eugene V Koonin; Detlef D Leipe; L Aravind
Journal: Nucleic Acids Res Date: 2005-07-15 Impact factor: 16.971

92 in total

10. The PSO4 protein complex associates with replication protein A (RPA) and modulates the activation of ataxia telangiectasia-mutated and Rad3-related (ATR).

Authors: Li Wan; Jun Huang
Journal: J Biol Chem Date: 2014-01-17 Impact factor: 5.157

hPrimpol1/CCDC111 is a human DNA primase-polymerase required for the maintenance of genome integrity.

Review 1. DNA primases.

2. Modulation of hyperthermophilic DNA polymerase activity by archaeal chromatin proteins.

3. A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activity.

4. Structure of a bifunctional DNA primase-polymerase.

5. ATR phosphorylates SMARCAL1 to prevent replication fork collapse.

Review 6. Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism.

7. Independent and coordinated functions of replication protein A tandem high affinity single-stranded DNA binding domains.

8. Key role of template sequence for primer synthesis by the herpes simplex virus 1 helicase-primase.

9. UV stalled replication forks restart by re-priming in human fibroblasts.

10. Origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural insights and new members.

Review 1. DNA replication stress: from molecular mechanisms to human disease.

2. Molecular dissection of the domain architecture and catalytic activities of human PrimPol.

3. Human Primpol1: a novel guardian of stalled replication forks.

Review 4. RPA-coated single-stranded DNA as a platform for post-translational modifications in the DNA damage response.

5. Deep-sea vent phage DNA polymerase specifically initiates DNA synthesis in the absence of primers.

6. Divalent Cations Alter the Rate-Limiting Step of PrimPol-Catalyzed DNA Elongation.

Review 7. Ribonucleotides in DNA: origins, repair and consequences.

Review 8. Mitochondrial genome maintenance in health and disease.

9. SLFN11 inhibits checkpoint maintenance and homologous recombination repair.

10. The PSO4 protein complex associates with replication protein A (RPA) and modulates the activation of ataxia telangiectasia-mutated and Rad3-related (ATR).