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Literature DB >> 2409961

The metabolism of neuropeptides. Endopeptidase-24.11 in human synaptic membrane preparations hydrolyses substance P.

R Matsas, M Rattray, A J Kenny, A J Turner.

Abstract

Synaptic membrane preparations from human striatum and human diencephalon were shown to contain a phosphoramidon-sensitive metalloendopeptidase that appeared identical with endopeptidase-24.11. The activity of endopeptidase-24.11 was determined with an enzymic assay employing [D-Ala2,Leu5]enkephalin as substrate, and its distribution in human brain was similar to that in pig brain, with the striatum containing the highest levels. The choroid plexus and pons also contained substantial activity. A good correlation (r = 0.97) was obtained for the distribution of the endopeptidase in pig brain and pituitary by the enzymic assay and by an immunoradiometric assay specific for pig endopeptidase-24.11. Synaptic membrane preparations from human striatum and diencephalon hydrolysed substance P at the same sites as did preparations of pig striatal synaptic membranes, and hydrolysis was substantially abolished by phosphoramidon. These results suggest that endopeptidase-24.11 is the principal enzyme hydrolysing substance P in human synaptic membrane preparations.

Entities: Chemical Gene Species

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Year: 1985 PMID： 2409961 PMCID： PMC1145007 DOI： 10.1042/bj2280487

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

12 in total

1. Subcellular distribution of particle-bound neutral peptidases capable of hydrolyzing gonadoliberin, thyroliberin, enkephalin and substance P.

Authors: B Horsthemke; P Leblanc; C Kordon; S Wattiaux-De Coninck; R Wattiaux; K Bauer
Journal: Eur J Biochem Date: 1984-03-01

2. Substance P and [Leu]enkephalin are hydrolyzed by an enzyme in pig caudate synaptic membranes that is identical with the endopeptidase of kidney microvilli.

Authors: R Matsas; I S Fulcher; A J Kenny; A J Turner
Journal: Proc Natl Acad Sci U S A Date: 1983-05 Impact factor: 11.205

3. A monoclonal antibody to kidney endopeptidase-24.11. Its application in immunoadsorbent purification of the enzyme and immunofluorescent microscopy of kidney and intestine.

Authors: N S Gee; R Matsas; A J Kenny
Journal: Biochem J Date: 1983-08-15 Impact factor: 3.857

4. Purification of endopeptidase-24.11 ('enkephalinase') from pig brain by immunoadsorbent chromatography.

Authors: J M Relton; N S Gee; R Matsas; A J Turner; A J Kenny
Journal: Biochem J Date: 1983-12-01 Impact factor: 3.857

5. Biochemical and immunological properties of a membrane-bound brain metalloendopeptidase: comparison with thermolysin-like kidney neutral metalloendopeptidase.

Authors: J Almenoff; M Orlowski
Journal: J Neurochem Date: 1984-01 Impact factor: 5.372

6. Kidney neutral endopeptidase and the hydrolysis of enkephalin by synaptic membranes show similar sensitivity to inhibitors.

Authors: I S Fulcher; R Matsas; A J Turner; A J Kenny
Journal: Biochem J Date: 1982-05-01 Impact factor: 3.857

7. Endopeptidase-24.11 and aminopeptidase activity in brain synaptic membranes are jointly responsible for the hydrolysis of cholecystokinin octapeptide (CCK-8).

Authors: R Matsas; A J Turner; A J Kenny
Journal: FEBS Lett Date: 1984-09-17 Impact factor: 4.124

8. A new feature of angiotensin-converting enzyme in the brain: hydrolysis of substance P.

Authors: H Yokosawa; S Endo; Y Ogura; S Ishii
Journal: Biochem Biophys Res Commun Date: 1983-10-31 Impact factor: 3.575

9. Carboxyl-terminal tripeptidyl hydrolysis of substance P by purified rabbit lung angiotensin-converting enzyme and the potentiation of substance P activity in vivo by captopril and MK-422.

Authors: M A Cascieri; H G Bull; R A Mumford; A A Patchett; N A Thornberry; T Liang
Journal: Mol Pharmacol Date: 1984-03 Impact factor: 4.436

10. Purification and characterisation of a membrane-bound substance-P-degrading enzyme from human brain.

Authors: C M Lee; B E Sandberg; M R Hanley; L L Iversen
Journal: Eur J Biochem Date: 1981-02

12 in total

1. Tachykininergic synaptic transmission in the coeliac ganglion of the guinea-pig.

Authors: F Y Zhao; K Saito; K Yoshioka; J Z Guo; T Murakoshi; S Konishi; M Otsuka
Journal: Br J Pharmacol Date: 1996-08 Impact factor: 8.739

Review 2. Sacubitril/valsartan in heart failure: latest evidence and place in therapy.

Authors: Edgardo Kaplinsky
Journal: Ther Adv Chronic Dis Date: 2016-10-19 Impact factor: 5.091

3. The metabolism of neuropeptides. Phase separation of synaptic membrane preparations with Triton X-114 reveals the presence of aminopeptidase N.

Authors: R Matsas; S L Stephenson; J Hryszko; A J Kenny; A J Turner
Journal: Biochem J Date: 1985-10-15 Impact factor: 3.857

9. The metabolism of neuropeptides. Neurokinin A (substance K) is a substrate for endopeptidase-24.11 but not for peptidyl dipeptidase A (angiotensin-converting enzyme).

Authors: N M Hooper; A J Kenny; A J Turner
Journal: Biochem J Date: 1985-10-15 Impact factor: 3.857

10. Isolation of two differentially glycosylated forms of peptidyl-dipeptidase A (angiotensin converting enzyme) from pig brain: a re-evaluation of their role in neuropeptide metabolism.

Authors: N M Hooper; A J Turner
Journal: Biochem J Date: 1987-02-01 Impact factor: 3.857