Literature DB >> 6419725

Purification of endopeptidase-24.11 ('enkephalinase') from pig brain by immunoadsorbent chromatography.

J M Relton, N S Gee, R Matsas, A J Turner, A J Kenny.   

Abstract

Membrane preparations from striatum of pig brain contain endopeptidase activity towards iodoinsulin B-chain. Only 50% of the hydrolysis of insulin B-chain is inhibitable by phosphoramidon, and DEAE-cellulose chromatography can resolve the phosphoramidon-sensitive and -insensitive activities. The former activity (now designated 'endopeptidase-24.11') is responsible for hydrolysis of [D-Ala2,Leu5]enkephalin and is identical with an enzyme in brain that has previously been referred to as 'enkephalinase'. Pig striatal endopeptidase-24.11 has now been purified to homogeneity in a single step by immunoadsorbent chromatography using a monoclonal antibody. The overall purification was 23 000-fold, with a yield of 30%. The brain enzyme appears to be identical with kidney endopeptidase-24.11 in amino acid composition as well as by immunological and kinetic criteria. However, it differs slightly in apparent subunit size (Mr = 87 000), attributable to differences in glycosylation.

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Year:  1983        PMID： 6419725      PMCID： PMC1152431          DOI： 10.1042/bj2150519

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  Two distinct enkephalinases: solubilization, partial purification and separation from angiotensin converting enzyme.

Authors:  C Gorenstein; S H Snyder
Journal:  Life Sci       Date:  1979 Dec 10-17       Impact factor: 5.037

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  In vitro degradation of the C-terminal octapeptide of cholecystokinin by 'enkephalinase A'.

Authors:  M Deschodt-Lanckman; A D Strosberg
Journal:  FEBS Lett       Date:  1983-02-07       Impact factor: 4.124

4.  Substance P and [Leu]enkephalin are hydrolyzed by an enzyme in pig caudate synaptic membranes that is identical with the endopeptidase of kidney microvilli.

Authors:  R Matsas; I S Fulcher; A J Kenny; A J Turner
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205

5.  A rapid and sensitive method for the analysis of carbohydrate components in glycoproteins using gas-liquid chromatography.

Authors:  M F Chaplin
Journal:  Anal Biochem       Date:  1982-07-01       Impact factor: 3.365

Review 6.  Microvillar endopeptidase, an enzyme with special topological features and a wide distribution.

Authors:  A J Kenny; I S Fulcher
Journal:  Ciba Found Symp       Date:  1983

7.  Membrane bound pituitary metalloendopeptidase: apparent identity to enkephalinase.

Authors:  J Almenoff; S Wilk; M Orlowski
Journal:  Biochem Biophys Res Commun       Date:  1981-09-16       Impact factor: 3.575

8.  Purification and characterization of a metallo-endoproteinase from mouse kidney.

Authors:  R J Beynon; J D Shannon; J S Bond
Journal:  Biochem J       Date:  1981-12-01       Impact factor: 3.857

9.  Microvillar membrane neutral endopeptidases.

Authors:  A J Kenny; I S Fulcher; K Ridgwell; J Ingram
Journal:  Acta Biol Med Ger       Date:  1981

10.  The enkephalinase inhibitor thiorphan shows antinociceptive activity in mice.

Authors:  B P Roques; M C Fournié-Zaluski; E Soroca; J M Lecomte; B Malfroy; C Llorens; J C Schwartz
Journal:  Nature       Date:  1980-11-20       Impact factor: 49.962
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  39 in total

1.  Ectoenzymes of the kidney microvillar membrane. Isolation and characterization of the amphipathic form of renal dipeptidase and hydrolysis of its glycosyl-phosphatidylinositol anchor by an activity in plasma.

Authors:  N M Hooper; A J Turner
Journal:  Biochem J       Date:  1989-08-01       Impact factor: 3.857

2.  Pig kidney angiotensin converting enzyme. Purification and characterization of amphipathic and hydrophilic forms of the enzyme establishes C-terminal anchorage to the plasma membrane.

Authors:  N M Hooper; J Keen; D J Pappin; A J Turner
Journal:  Biochem J       Date:  1987-10-01       Impact factor: 3.857

3.  The metabolism of neuropeptides. Endopeptidase-24.11 in human synaptic membrane preparations hydrolyses substance P.

Authors:  R Matsas; M Rattray; A J Kenny; A J Turner
Journal:  Biochem J       Date:  1985-06-01       Impact factor: 3.857

4.  cDNA cloning and expression in Xenopus laevis oocytes of pig renal dipeptidase, a glycosyl-phosphatidylinositol-anchored ectoenzyme.

Authors:  E Rached; N M Hooper; P James; G Semenza; A J Turner; N Mantei
Journal:  Biochem J       Date:  1990-11-01       Impact factor: 3.857

Review 5.  Regulatory mechanisms that modulate signalling by G-protein-coupled receptors.

Authors:  S K Böhm; E F Grady; N W Bunnett
Journal:  Biochem J       Date:  1997-02-15       Impact factor: 3.857

6.  The metabolism of neuropeptides. Both phosphoramidon-sensitive and captopril-sensitive metallopeptidases are present in the electric organ of Torpedo marmorata.

Authors:  A J Turner; M J Dowdall
Journal:  Biochem J       Date:  1984-08-15       Impact factor: 3.857

7.  The metabolism of neuropeptides. Phase separation of synaptic membrane preparations with Triton X-114 reveals the presence of aminopeptidase N.

Authors:  R Matsas; S L Stephenson; J Hryszko; A J Kenny; A J Turner
Journal:  Biochem J       Date:  1985-10-15       Impact factor: 3.857

8.  Characterization of neutral endopeptidase 24.11 in dog glomeruli.

Authors:  C Landry; P Santagata; W Bawab; M C Fournié-Zaluski; B P Roques; P Vinay; P Crine
Journal:  Biochem J       Date:  1993-05-01       Impact factor: 3.857

9.  Endopeptidase-24.11 in pig lymph nodes. Purification and immunocytochemical localization in reticular cells.

Authors:  M A Bowes; A J Kenny
Journal:  Biochem J       Date:  1986-06-15       Impact factor: 3.857

10.  A comparison of the zinc contents and substrate specificities of the endothelial and testicular forms of porcine angiotensin converting enzyme and the preparation of isoenzyme-specific antisera.

Authors:  T A Williams; K Barnes; A J Kenny; A J Turner; N M Hooper
Journal:  Biochem J       Date:  1992-12-15       Impact factor: 3.857
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