Jaime Sánchez-López1, Leticia Tordesillas2, Mariona Pascal3, Rosa Muñoz-Cano4, María Garrido2, Maria Rueda4, Ramón Vilella3, Antonio Valero4, Araceli Díaz-Perales2, César Picado4, Joan Bartra4. 1. Unitat d'Al·lèrgia, Institut Clínic del Tòrax (ICT), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain. Electronic address: jasanche@clinic.ub.es. 2. Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid, Campus Montegancedo, Madrid, Spain. 3. Servei d'Immunologia, Centre de Diagnòstic Biomèdic (CDB), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain. 4. Unitat d'Al·lèrgia, Institut Clínic del Tòrax (ICT), Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
Abstract
BACKGROUND: Food allergy caused by lipid transfer protein (LTP) from peach (Pru p 3) is frequently associated with sensitization to mugwort LTP (Art v 3). Although in vitro cross-reactivity is already well known, it has yet to be elucidated whether a pollen LTP can induce rhinitis. OBJECTIVE: The aim of this study was to investigate whether mugwort LTP could elicit respiratory symptoms and whether a primary food LTP allergy could lead to a respiratory allergy. METHODS: Patients with confirmed Pru p 3 allergy and control subjects were selected. Immediate responses to nasal allergen provocation tests (NAPTs) with Art v 3, Pru p 3, and mugwort were assessed by using the visual analog scale score, total nasal symptom score, and acoustic rhinometry. Tryptase and cysteinyl leukotriene (cysLT) levels were measured in nasal lavage fluid. Immunoblotting, ELISAs, and ELISA inhibition assays were also performed. RESULTS: Fifteen patients and 9 control subjects were selected. NAPT results with Art v 3 and Pru p 3 showed significant changes in acoustic rhinometry, visual analog scale scores, total nasal symptom scores, and cysLT levels (P < .001). Tryptase levels were only increased in NAPTs with Pru p 3. NAPTs with mugwort were used in those patients who were only sensitized to Art v 3, with similar results (P < .05). No significant changes were detected in control subjects. CONCLUSION: The results demonstrated that a pollen LTP can elicit rhinitis in sensitized patients. Findings also suggest that a primary sensitization to Pru p 3 can lead to a respiratory allergy through cross-reactivity.
BACKGROUND: Food allergy caused by lipid transfer protein (LTP) from peach (Pru p 3) is frequently associated with sensitization to mugwort LTP (Art v 3). Although in vitro cross-reactivity is already well known, it has yet to be elucidated whether a pollen LTP can induce rhinitis. OBJECTIVE: The aim of this study was to investigate whether mugwort LTP could elicit respiratory symptoms and whether a primary food LTP allergy could lead to a respiratory allergy. METHODS:Patients with confirmed Pru p 3allergy and control subjects were selected. Immediate responses to nasal allergen provocation tests (NAPTs) with Art v 3, Pru p 3, and mugwort were assessed by using the visual analog scale score, total nasal symptom score, and acoustic rhinometry. Tryptase and cysteinyl leukotriene (cysLT) levels were measured in nasal lavage fluid. Immunoblotting, ELISAs, and ELISA inhibition assays were also performed. RESULTS: Fifteen patients and 9 control subjects were selected. NAPT results with Art v 3 and Pru p 3 showed significant changes in acoustic rhinometry, visual analog scale scores, total nasal symptom scores, and cysLT levels (P < .001). Tryptase levels were only increased in NAPTs with Pru p 3. NAPTs with mugwort were used in those patients who were only sensitized to Art v 3, with similar results (P < .05). No significant changes were detected in control subjects. CONCLUSION: The results demonstrated that a pollen LTP can elicit rhinitis in sensitized patients. Findings also suggest that a primary sensitization to Pru p 3 can lead to a respiratory allergy through cross-reactivity.
Authors: Isabel J Skypala; Ricardo Asero; Domingo Barber; Lorenzo Cecchi; Arazeli Diaz Perales; Karin Hoffmann-Sommergruber; Elide A Pastorello; Ines Swoboda; Joan Bartra; Didier G Ebo; Margaretha A Faber; Montserrat Fernández-Rivas; Francesca Gomez; Anastasios P Konstantinopoulos; Olga Luengo; Ronald van Ree; Enrico Scala; Stephen J Till Journal: Clin Transl Allergy Date: 2021-05-18 Impact factor: 5.871
Authors: Maria J Rodriguez; Ana Aranda; Tahia D Fernandez; Nuria Cubells-Baeza; Maria J Torres; Francisca Gomez; Francisca Palomares; James R Perkins; Javier Rojo; Araceli Diaz-Perales; Cristobalina Mayorga Journal: Sci Rep Date: 2017-01-13 Impact factor: 4.379