Literature DB >> 24056008

Revised structures for the predominant O-polysaccharides expressed by Burkholderia pseudomallei and Burkholderia mallei.

Christian Heiss1, Mary N Burtnick, Rosemary A Roberts, Ian Black, Parastoo Azadi, Paul J Brett.   

Abstract

O-Polysaccharides (OPS) were isolated from purified Burkholderia pseudomallei and Burkholderia mallei lipopolysaccharides by mild-acid hydrolysis and gel-permeation chromatography. 1-D and 2-D (1)H and (13)C NMR spectroscopy experiments revealed that the OPS antigens were unbranched heteropolymers with the following structures: Collectively, our results demonstrate that the predominant OPS antigens expressed by B. pseudomallei and B. mallei isolates are structurally more complex than previously described and provide evidence that different capping residues are used by these closely related pathogens to terminate chain elongation. Additionally, they confirm that Burkholderia thailandensis and B. pseudomallei express OPS antigens that are essentially identical to one another.
Copyright © 2013 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Burkholderia mallei; Burkholderia pseudomallei; Burkholderia thailandensis; Lipopolysaccharide; O-polysaccharide; Structure

Mesh:

Substances:

Year:  2013        PMID: 24056008      PMCID: PMC3923507          DOI: 10.1016/j.carres.2013.08.013

Source DB:  PubMed          Journal:  Carbohydr Res        ISSN: 0008-6215            Impact factor:   2.104


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