Literature DB >> 24038733

Folding of 16S rRNA in a signal-producing structure for the detection of bacteria.

Yulia V Gerasimova1, Dmitry M Kolpashchikov.   

Abstract

Sixty-four DNA strands hybridize to 16S rRNA to form 32 deoxyribozyme catalytic cores that produce a fluorescent signal. The approach allows detection of 0.6 pM 16S rRNA, or about 3×10(4) bacterial cells in a PCR-free format.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  16S rRNA; DNA origami; binary deoxyribozyme; detection of bacteria; fluorescent sensors

Mesh：

Substances：

Year:  2013        PMID： 24038733      PMCID： PMC3863991          DOI： 10.1002/anie.201303919

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


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