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Literature DB >> 239681

The electrophoretic mobilities of 5-dimethylaminoaphthalene-1-suphonyl-glycopeptides and their relation molecular weight.

Abstract

The relationship between the electrophoretic mobility at pH2.1 of dansyl-glycopeptides of known composition and their molecular weight is shown to conform with a model equation previously derived for peptides. A dansyl-glycopeptide prepared from hen's-egg ovotransferrin is degraded sequentially with two glycosidases. The molecular weight of each glycopeptide intermediate formed is determined from its electrophoretic mobility. From successive molecular-weight changes, the number and type of sugar residues lost from the parent glycopeptide can be decided and the probable composition of each intermediate determined. The notion that the method has considerable application and would permit analysis of very small quantities of glycopeptides is discussed.

Entities: Chemical

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Year: 1975 PMID： 239681 PMCID： PMC1165216 DOI： 10.1042/bj1450281

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

7. IUPAC-IUB Commission on Biochemical Nomenclature. Rules for naming synthetic modifications of natural peptides. Tentative rules.

Authors:
Journal: Biochem J Date: 1967-07 Impact factor: 3.857

2. The use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labelled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid. Detection of picomolar quantities by an imaging system based on a cooled charge-coupled device.

Authors: P Jackson
Journal: Biochem J Date: 1990-09-15 Impact factor: 3.857

The electrophoretic mobilities of 5-dimethylaminoaphthalene-1-suphonyl-glycopeptides and their relation molecular weight.

1. EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS.

2. THE AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551.

3. Determination of serum glycoproteins.

4. The determination of glucosamine and galactosamine.

5. Studies on glucosaminidase. 2. Substrates for N-acetyl-beta-glucosaminidase.

6. Reagent for Differentiation of 1,4- and 1,6-Linked Glucosaccharides.

7. IUPAC-IUB Commission on Biochemical Nomenclature. Rules for naming synthetic modifications of natural peptides. Tentative rules.

8. The electrophoretic mobility of peptides on paper at pH 1.9.

9. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups.

10. A comparison of glycopeptides from the ovotransferrin and serum transferrin of the hen.

1. The amino acid sequence of a carbohydrate-containing fragment of hen ovotransferrin.

2. The use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labelled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid. Detection of picomolar quantities by an imaging system based on a cooled charge-coupled device.

3. A comparsion of glycopeptides from the transferrins of several species.

4. Iron-binding fragments from the carboxyl-terminal region of hen ovotransferrin.

Review 5. The analysis of fluorophore-labeled carbohydrates by polyacrylamide gel electrophoresis.