Amina M Bhatia1, Barbara J Stoll2, Mary J Cismowski3, Shannon E Hamrick2. 1. Department of Surgery, Children's Healthcare of Atlanta, Emory University School of Medicine, Atlanta, Georgia. 2. Department of Pediatrics, Children's Healthcare of Atlanta, Emory University School of Medicine, Atlanta, Georgia. 3. Center for Cardiovascular and Pulmonary Research, Nationwide Children's Hospital, Columbus, Ohio.
Abstract
OBJECTIVES: The purpose of this study is to characterize the cytokine response of preterm newborns with surgical necrotizing enterocolitis (NEC) or spontaneous intestinal perforation (SIP) before surgical treatment and to relate these finding to intestinal disease (NEC vs. SIP). STUDY DESIGN: The study was a 14-month prospective, cohort study of neonates undergoing surgery or drainage for NEC or SIP or surgical ligation of patent ductus arteriosus (PDA). Multiplex cytokine detection technology was used to analyze six inflammatory markers: interleukin-2, interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-1 β (IL-1β), interferon-gamma, and tumor necrosis factor-α (TNF-α). RESULTS: Patients with NEC had much higher median preoperative levels of IL-6 (NEC: 8,381 pg/mL; SIP: 36 pg/mL; PDA: 25 pg/mL, p < 0.001), IL-8 (NEC: 18,438 pg/mL; SIP: 2,473 pg/mL; PDA: 1,110 pg/mL, p = 0.001), TNF-α (NEC: 161 pg/mL; SIP: 77 pg/mL; PDA: 71 pg/mL, p < 0.001), and IL-1β (NEC: 85 pg/mL; SIP: 31 pg/mL; PDA: 24 pg/mL, p = 0.001). Patients with NEC totalis (NEC-totalis had the highest levels of IL-8 and were significantly different from infants with limited NEC (28,141 vs. 11,429 pg/mL, p = 0.03). CONCLUSION: Surgical NEC is a profoundly more proinflammatory disease than SIP. The cytokine profiles of patients with SIP are closer to those of a nonseptic surgical neonate. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.
OBJECTIVES: The purpose of this study is to characterize the cytokine response of preterm newborns with surgical necrotizing enterocolitis (NEC) or spontaneous intestinal perforation (SIP) before surgical treatment and to relate these finding to intestinal disease (NEC vs. SIP). STUDY DESIGN: The study was a 14-month prospective, cohort study of neonates undergoing surgery or drainage for NEC or SIP or surgical ligation of patent ductus arteriosus (PDA). Multiplex cytokine detection technology was used to analyze six inflammatory markers: interleukin-2, interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-1 β (IL-1β), interferon-gamma, and tumor necrosis factor-α (TNF-α). RESULTS:Patients with NEC had much higher median preoperative levels of IL-6 (NEC: 8,381 pg/mL; SIP: 36 pg/mL; PDA: 25 pg/mL, p < 0.001), IL-8 (NEC: 18,438 pg/mL; SIP: 2,473 pg/mL; PDA: 1,110 pg/mL, p = 0.001), TNF-α (NEC: 161 pg/mL; SIP: 77 pg/mL; PDA: 71 pg/mL, p < 0.001), and IL-1β (NEC: 85 pg/mL; SIP: 31 pg/mL; PDA: 24 pg/mL, p = 0.001). Patients with NEC totalis (NEC-totalis had the highest levels of IL-8 and were significantly different from infants with limited NEC (28,141 vs. 11,429 pg/mL, p = 0.03). CONCLUSION: Surgical NEC is a profoundly more proinflammatory disease than SIP. The cytokine profiles of patients with SIP are closer to those of a nonseptic surgical neonate. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.
Authors: Krishnan MohanKumar; Kopperuncholan Namachivayam; Thao T B Ho; Benjamin A Torres; Robin K Ohls; Akhil Maheshwari Journal: Semin Perinatol Date: 2016-11-07 Impact factor: 3.300
Authors: Timothy L Denning; Amina M Bhatia; Andrea F Kane; Ravi M Patel; Patricia W Denning Journal: Semin Perinatol Date: 2016-12-09 Impact factor: 3.300
Authors: Cesar M Rueda; Pietro Presicce; Courtney M Jackson; Lisa A Miller; Suhas G Kallapur; Alan H Jobe; Claire A Chougnet Journal: J Immunol Date: 2016-04-01 Impact factor: 5.422
Authors: Oluwabunmi O Olaloye; Peng Liu; Jessica M Toothaker; Blake T McCourt; Collin C McCourt; Jenny Xiao; Erica Prochaska; Spenser Shaffer; Lael Werner; Jordan Gringauz; Misty Good; Jeffrey D Goldsmith; Xiaojing An; Fujing Wang; Scott B Snapper; Dror Shouval; Kong Chen; George Tseng; Liza Konnikova Journal: J Exp Med Date: 2021-07-16 Impact factor: 14.307