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Literature DB >> 2395634

The role of modified purine 64 in initiator/elongator discrimination of tRNA(iMet) from yeast and wheat germ.

Abstract

The role of 2'-ribosylated adenosine 64 in tRNA(iMet) from yeast in initiation/elongation discrimination was investigated. As measured by in vitro translation in rabbit reticulocyte lysate, the specific removal of the 2'-ribosylphosphate at adenosine 64 via periodate oxidation allows tRNA(iMet) to read internal AUG codons of the globine messenger RNA. Yeast Met-tRNA(iMet) lacking the modification of nucleoside 64 forms ternary complexes with GTP and elongation factor Tu from Escherichia coli. The lack of modification at position 64 does not prevent tRNA(iMet) from participating in the initiation process of in vitro protein synthesis. Wheat germ tRNA(iMet) has a 2'-ribosylated guanosine at position 64. Removal of this modification from the wheat germ tRNA(iMet) enables it to read internal AUG codons of globine and tobacco mosaic virus messenger RNA in reticulocyte and wheat germ translation systems, respectively.

Entities: Chemical Gene Species

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Year: 1990 PMID： 2395634 PMCID： PMC331916 DOI： 10.1093/nar/18.16.4677

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

22 in total

1. Presence of phosphorylated O-ribosyl-adenosine in T-psi-stem of yeast methionine initiator tRNA.

Authors: J Desgrès; G Keith; K C Kuo; C W Gehrke
Journal: Nucleic Acids Res Date: 1989-02-11 Impact factor: 16.971

2. Interaction of elongation factor Tu from Escherichia coli with aminoacyl-tRNA carrying a fluorescent reporter group on the 3' terminus.

Authors: G Ott; H G Faulhammer; M Sprinzl
Journal: Eur J Biochem Date: 1989-09-15

3. Crystal structure of a eukaryotic initiator tRNA.

Authors: R W Schevitz; A D Podjarny; N Krishnamachari; J J Hughes; P B Sigler; J L Sussman
Journal: Nature Date: 1979-03-08 Impact factor: 49.962

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Authors: D Schneider; R Solfert; F von der Haar
Journal: Hoppe Seylers Z Physiol Chem Date: 1972-08

5. Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients.

Authors: W M Holmes; R E Hurd; B R Reid; R A Rimerman; G W Hatfield
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

6. Rapid analysis of modified tRNAphe from yeast by high-performance liquid chromatography: chromatography of oligonucleotides after RNase T1 digestion on aminopropylsilica and assignment of the fragments based on nucleoside analysis by chromatography on C18-silica.

Authors: L W McLaughlin; F Cramer; M Sprinzl
Journal: Anal Biochem Date: 1981-03-15 Impact factor: 3.365

7. Expression in vivo of a mutant human initiator tRNA gene in mammalian cells using a simian virus 40 vector.

Authors: H J Drabkin; U L RajBhandary
Journal: J Biol Chem Date: 1985-05-10 Impact factor: 5.157

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Authors: M Sprinzl; H Sternbach; F von der Haar; F Cramer
Journal: Eur J Biochem Date: 1977-12

9. Escherichia coli formylmethionine tRNA: mutations in GGGCCC sequence conserved in anticodon stem of initiator tRNAs affect initiation of protein synthesis and conformation of anticodon loop.

Authors: B L Seong; U L RajBhandary
Journal: Proc Natl Acad Sci U S A Date: 1987-01 Impact factor: 11.205

10. Suppression of amber codons in vivo as evidence that mutants derived from Escherichia coli initiator tRNA can act at the step of elongation in protein synthesis.

Authors: B L Seong; C P Lee; U L RajBhandary
Journal: J Biol Chem Date: 1989-04-15 Impact factor: 5.157

26 in total

1. The yeast retrotransposon Ty5 uses the anticodon stem-loop of the initiator methionine tRNA as a primer for reverse transcription.

Authors: N Ke; X Gao; J B Keeney; J D Boeke; D F Voytas
Journal: RNA Date: 1999-07 Impact factor: 4.942

2. Preparation and activity of synthetic unmodified mammalian tRNAi(Met) in initiation of translation in vitro.

Authors: T V Pestova; C U Hellen
Journal: RNA Date: 2001-10 Impact factor: 4.942

3. A critical role of water in the specific cleavage of the anticodon loop of some eukaryotic methionine initiator tRNAs.

Authors: Marcus Perbandt; Miroslawa Z Barciszewska; Christian Betzel; Volker A Erdmann; Jan Barciszewski
Journal: Mol Biol Rep Date: 2003-03 Impact factor: 2.316

4. Atypical archaeal tRNA pyrrolysine transcript behaves towards EF-Tu as a typical elongator tRNA.

Authors: Anne Théobald-Dietrich; Magali Frugier; Richard Giegé; Joëlle Rudinger-Thirion
Journal: Nucleic Acids Res Date: 2004-02-10 Impact factor: 16.971

5. Identification and modeling of a phosphatase-like domain in a tRNA 2'-O-ribosyl phosphate transferase Rit1p.

Authors: Anna Czerwoniec; Janusz M Bujnicki
Journal: Cell Cycle Date: 2011-10-15 Impact factor: 4.534

6. How are tRNAs and mRNA arranged in the ribosome? An attempt to correlate the stereochemistry of the tRNA-mRNA interaction with constraints imposed by the ribosomal topography.

Authors: V Lim; C Venclovas; A Spirin; R Brimacombe; P Mitchell; F Müller
Journal: Nucleic Acids Res Date: 1992-06-11 Impact factor: 16.971

10. Mutational analysis of conserved positions potentially important for initiator tRNA function in Saccharomyces cerevisiae.

Authors: U von Pawel-Rammingen; S Aström; A S Byström
Journal: Mol Cell Biol Date: 1992-04 Impact factor: 4.272