Yanyan Li1, Tao Zhang, Xiaoqin Li, Peng Zou, Steven J Schwartz, Duxin Sun. 1. Department of Health and Nutrition Sciences, Montclair State University, Montclair, NJ, USA; Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI, USA; Department of Food Science and Technology, Ohio State University, Columbus, OH, USA.
Abstract
SCOPE: Sulforaphane (SF) is a natural isothiocyanate in broccoli sprouts with cancer chemopreventive activity. This study is aimed to use different methods to develop broccoli sprout preparations to compare their ability to deliver SF to the mice and to evaluate the kinetics and biodistribution of SF. METHODS AND RESULTS: The SF-enriched sprout preparation generated by two-step procedure (quick-steaming followed by myrosinase treatment) contained the highest level of SF, which was 11 and 5 times higher than the freeze-dried fresh broccoli sprouts and the quick-steamed, freeze-dried broccoli sprouts, respectively. After oral administration of 2.5 mg/g body weight of the broccoli sprout preparations, SF was quickly absorbed and distributed throughout the tissues. The SF-rich preparation resulted in the highest exposure, with peak plasma SF concentration of 337 ng/mL, which is 6.0 times and 2.6 times higher compared to the other two preparations. A whole body physiologically based pharmacokinetic model (developed with ADAPT 5 software) suggests that distribution of SF is perfusion-limited in all organs. CONCLUSION: This study provides a broccoli sprout preparation that can serve as a good source of SF, and the model can be utilized to guide the dose designed for the use of broccoli sprout preparation in chemoprevention.
SCOPE: Sulforaphane (SF) is a natural isothiocyanate in broccoli sprouts with cancer chemopreventive activity. This study is aimed to use different methods to develop broccoli sprout preparations to compare their ability to deliver SF to the mice and to evaluate the kinetics and biodistribution of SF. METHODS AND RESULTS: The SF-enriched sprout preparation generated by two-step procedure (quick-steaming followed by myrosinase treatment) contained the highest level of SF, which was 11 and 5 times higher than the freeze-dried fresh broccoli sprouts and the quick-steamed, freeze-dried broccoli sprouts, respectively. After oral administration of 2.5 mg/g body weight of the broccoli sprout preparations, SF was quickly absorbed and distributed throughout the tissues. The SF-rich preparation resulted in the highest exposure, with peak plasma SF concentration of 337 ng/mL, which is 6.0 times and 2.6 times higher compared to the other two preparations. A whole body physiologically based pharmacokinetic model (developed with ADAPT 5 software) suggests that distribution of SF is perfusion-limited in all organs. CONCLUSION: This study provides a broccoli sprout preparation that can serve as a good source of SF, and the model can be utilized to guide the dose designed for the use of broccoli sprout preparation in chemoprevention.
Authors: C C Conaway; S M Getahun; L L Liebes; D J Pusateri; D K Topham; M Botero-Omary; F L Chung Journal: Nutr Cancer Date: 2000 Impact factor: 2.900
Authors: Andreas Ratzka; Heiko Vogel; Daniel J Kliebenstein; Thomas Mitchell-Olds; Juergen Kroymann Journal: Proc Natl Acad Sci U S A Date: 2002-08-02 Impact factor: 11.205
Authors: Niclas Petri; Christer Tannergren; Birgit Holst; Fred A Mellon; Yongping Bao; Geoff W Plumb; Jim Bacon; Karen A O'Leary; Paul A Kroon; Lars Knutson; Patrik Forsell; Thomas Eriksson; Hans Lennernas; Gary Williamson Journal: Drug Metab Dispos Date: 2003-06 Impact factor: 3.922
Authors: Jed W Fahey; Xavier Haristoy; Patrick M Dolan; Thomas W Kensler; Isabelle Scholtus; Katherine K Stephenson; Paul Talalay; Alain Lozniewski Journal: Proc Natl Acad Sci U S A Date: 2002-05-28 Impact factor: 11.205
Authors: Ellen A Rorke; Gautam Adhikary; Henryk Szmacinski; Joseph R Lakowicz; David J Weber; Raquel Godoy-Ruiz; Purushottamachar Puranik; Jeffrey W Keillor; Eric W J Gates; Richard L Eckert Journal: Mol Carcinog Date: 2021-10-05 Impact factor: 4.784
Authors: Andrea Kinga Marias Furuya; Hamayun J Sharifi; Robert M Jellinger; Paul Cristofano; Binshan Shi; Carlos M C de Noronha Journal: PLoS Pathog Date: 2016-04-19 Impact factor: 6.823