Rawee Teanpaisan1, Supatcharin Piwat. 1. Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, 90112, Thailand, rawee.t@psu.ac.th.
Abstract
OBJECTIVES: The present study investigates the effect of an oral Lactobacillus paracasei SD1 isolate on a number of salivary mutans streptococci, lactobacilli, and yeasts and to examine whether the strain could persist in the oral cavity after a short period of consumption. The potential side effect of the probiotic was also evaluated. MATERIAL AND METHODS: After informed consent, 40 healthy young adults were recruited and randomly assigned to either the test (A) or placebo (B) groups. Group A received milk powdercontained L. paracasei SD1, and group B received standard milk powder once daily for 4 weeks. Salivary mutans streptococci, lactobacilli, and yeasts were enumerated using quantitative differential culture at the baseline and then once a week for 4 weeks after the administration period. The persistence of L. paracasei SD1 was also investigated using AP-PCR for the DNA fingerprinting. RESULTS: A statistically significant reduction in mutans streptococci counts occurred within group A compared to the baseline B (P < 0.05) along with a non-statistically significant reduction in yeast counts. A significant increase in lactobacilli numbers occurred in group A (P < 0.05), and the probiotic strain could be detected up to 4 weeks following cessation of dosing. CONCLUSIONS: Our results demonstrate that short-term daily ingestion of the human-derived probiotic L. paracasei SD1 reduces the number of oral mutans streptococci. CLINICAL RELEVANCE: L. paracasei SD1 may help to decrease the risk of dental caries, and this organism may be a useful probiotic organism for dairy products.
RCT Entities:
OBJECTIVES: The present study investigates the effect of an oral Lactobacillus paracaseiSD1 isolate on a number of salivary mutans streptococci, lactobacilli, and yeasts and to examine whether the strain could persist in the oral cavity after a short period of consumption. The potential side effect of the probiotic was also evaluated. MATERIAL AND METHODS: After informed consent, 40 healthy young adults were recruited and randomly assigned to either the test (A) or placebo (B) groups. Group A received milk powder contained L. paracaseiSD1, and group B received standard milk powder once daily for 4 weeks. Salivary mutans streptococci, lactobacilli, and yeasts were enumerated using quantitative differential culture at the baseline and then once a week for 4 weeks after the administration period. The persistence of L. paracaseiSD1 was also investigated using AP-PCR for the DNA fingerprinting. RESULTS: A statistically significant reduction in mutans streptococci counts occurred within group A compared to the baseline B (P < 0.05) along with a non-statistically significant reduction in yeast counts. A significant increase in lactobacilli numbers occurred in group A (P < 0.05), and the probiotic strain could be detected up to 4 weeks following cessation of dosing. CONCLUSIONS: Our results demonstrate that short-term daily ingestion of the human-derived probiotic L. paracaseiSD1 reduces the number of oral mutans streptococci. CLINICAL RELEVANCE: L. paracaseiSD1 may help to decrease the risk of dental caries, and this organism may be a useful probiotic organism for dairy products.
Authors: Juçara R Franca; Mariana P De Luca; Tatiana G Ribeiro; Rachel O Castilho; Allyson N Moreira; Vagner R Santos; André A G Faraco Journal: BMC Complement Altern Med Date: 2014-12-12 Impact factor: 3.659