| Literature DB >> 23843942 |
Federica Villanova1, Paola Di Meglio, Margaret Inokuma, Nima Aghaeepour, Esperanza Perucha, Jennifer Mollon, Laurel Nomura, Maria Hernandez-Fuentes, Andrew Cope, A Toby Prevost, Susanne Heck, Vernon Maino, Graham Lord, Ryan R Brinkman, Frank O Nestle.
Abstract
Discovery of novel immune biomarkers for monitoring of disease prognosis and response to therapy in immune-mediated inflammatory diseases is an important unmet clinical need. Here, we establish a novel framework for immunological biomarker discovery, comparing a conventional (liquid) flow cytometry platform (CFP) and a unique lyoplate-based flow cytometry platform (LFP) in combination with advanced computational data analysis. We demonstrate that LFP had higher sensitivity compared to CFP, with increased detection of cytokines (IFN-γ and IL-10) and activation markers (Foxp3 and CD25). Fluorescent intensity of cells stained with lyophilized antibodies was increased compared to cells stained with liquid antibodies. LFP, using a plate loader, allowed medium-throughput processing of samples with comparable intra- and inter-assay variability between platforms. Automated computational analysis identified novel immunophenotypes that were not detected with manual analysis. Our results establish a new flow cytometry platform for standardized and rapid immunological biomarker discovery with wide application to immune-mediated diseases.Entities:
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Year: 2013 PMID： 23843942 PMCID： PMC3701052 DOI： 10.1371/journal.pone.0065485
Source DB: PubMed Journal: PLoS One ISSN： 1932-6203 Impact factor: 3.240