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Literature DB >> 23839075

A fluorogenic assay for screening Sirt6 modulators.

Jing Hu¹, Bin He, Shiva Bhargava, Hening Lin.

Abstract

A fluorogenic high-throughput assay suitable for screening Sirt6 modulators is developed based on the recently discovered efficient activity of Sirt6 to hydrolyze myristoyl lysine. Sirt6 modulators will be useful in investigating the function of Sirt6 and protein lysine fatty acylation.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 2013 PMID： 23839075 PMCID： PMC3756594 DOI： 10.1039/c3ob41138a

Source DB: PubMed Journal: Org Biomol Chem ISSN： 1477-0520 Impact factor: 3.876

35 in total

1. Identification of a class of small molecule inhibitors of the sirtuin family of NAD-dependent deacetylases by phenotypic screening.

Authors: C M Grozinger; E D Chao; H E Blackwell; D Moazed; S L Schreiber
Journal: J Biol Chem Date: 2001-08-01 Impact factor: 5.157

2. A fluorogenic histone deacetylase assay well suited for high-throughput activity screening.

Authors: Dennis Wegener; Frank Wirsching; Daniel Riester; Andreas Schwienhorst
Journal: Chem Biol Date: 2003-01

3. Sirt1 inhibitor, Sirtinol, induces senescence-like growth arrest with attenuated Ras-MAPK signaling in human cancer cells.

Authors: H Ota; E Tokunaga; K Chang; M Hikasa; K Iijima; M Eto; K Kozaki; M Akishita; Y Ouchi; M Kaneki
Journal: Oncogene Date: 2006-01-12 Impact factor: 9.867

4. Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1.

Authors: Kevin J Bitterman; Rozalyn M Anderson; Haim Y Cohen; Magda Latorre-Esteves; David A Sinclair
Journal: J Biol Chem Date: 2002-09-23 Impact factor: 5.157

5. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase.

Authors: S Imai; C M Armstrong; M Kaeberlein; L Guarente
Journal: Nature Date: 2000-02-17 Impact factor: 49.962

6. Mechanism of human SIRT1 activation by resveratrol.

Authors: Margie T Borra; Brian C Smith; John M Denu
Journal: J Biol Chem Date: 2005-03-04 Impact factor: 5.157

7. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins.

Authors: Eriko Michishita; Jean Y Park; Jenna M Burneskis; J Carl Barrett; Izumi Horikawa
Journal: Mol Biol Cell Date: 2005-08-03 Impact factor: 4.138

8. Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan.

Authors: Konrad T Howitz; Kevin J Bitterman; Haim Y Cohen; Dudley W Lamming; Siva Lavu; Jason G Wood; Robert E Zipkin; Phuong Chung; Anne Kisielewski; Li-Li Zhang; Brandy Scherer; David A Sinclair
Journal: Nature Date: 2003-08-24 Impact factor: 49.962

9. Antitumor effect of SIRT1 inhibition in human HCC tumor models in vitro and in vivo.

Authors: Simone Portmann; René Fahrner; Antje Lechleiter; Adrian Keogh; Sarah Overney; Alexander Laemmle; Kei Mikami; Matteo Montani; Mario P Tschan; Daniel Candinas; Deborah Stroka
Journal: Mol Cancer Ther Date: 2013-01-21 Impact factor: 6.261

10. Fluorescence assay of SIRT protein deacetylases using an acetylated peptide substrate and a secondary trypsin reaction.

Authors: Patrick A Marcotte; Paul L Richardson; Paul R Richardson; Jun Guo; Leo W Barrett; Nan Xu; Angelo Gunasekera; Keith B Glaser
Journal: Anal Biochem Date: 2004-09-01 Impact factor: 3.365

18 in total

1. Sirtuin 6 (SIRT6) Activity Assays.

Authors: Minna Rahnasto-Rilla; Maija Lahtela-Kakkonen; Ruin Moaddel
Journal: Methods Mol Biol Date: 2016

2. Fluorogenic Assays for the Defatty-Acylase Activity of Sirtuins.

Authors: Jun Young Hong; Ji Cao; Hening Lin
Journal: Methods Mol Biol Date: 2019

3. Investigating the Sensitivity of NAD+-dependent Sirtuin Deacylation Activities to NADH.

Authors: Andreas S Madsen; Christian Andersen; Mohammad Daoud; Kristin A Anderson; Jonas S Laursen; Saswati Chakladar; Frank K Huynh; Ana R Colaço; Donald S Backos; Peter Fristrup; Matthew D Hirschey; Christian A Olsen
Journal: J Biol Chem Date: 2016-02-09 Impact factor: 5.157

A fluorogenic assay for screening Sirt6 modulators.

1. Identification of a class of small molecule inhibitors of the sirtuin family of NAD-dependent deacetylases by phenotypic screening.

2. A fluorogenic histone deacetylase assay well suited for high-throughput activity screening.

3. Sirt1 inhibitor, Sirtinol, induces senescence-like growth arrest with attenuated Ras-MAPK signaling in human cancer cells.

4. Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1.

5. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase.

6. Mechanism of human SIRT1 activation by resveratrol.

7. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins.

8. Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan.

9. Antitumor effect of SIRT1 inhibition in human HCC tumor models in vitro and in vivo.

10. Fluorescence assay of SIRT protein deacetylases using an acetylated peptide substrate and a secondary trypsin reaction.

1. Sirtuin 6 (SIRT6) Activity Assays.

2. Fluorogenic Assays for the Defatty-Acylase Activity of Sirtuins.

3. Investigating the Sensitivity of NAD+-dependent Sirtuin Deacylation Activities to NADH.

Review 4. SIRT6: Novel Mechanisms and Links to Aging and Disease.

5. The sirtuin family in cancer.

Review 6. Sirtuin inhibitors as anticancer agents.

Review 7. Natural compound-derived epigenetic regulators targeting epigenetic readers, writers and erasers.

8. An improved fluorogenic assay for SIRT1, SIRT2, and SIRT3.

9. Thiomyristoyl peptides as cell-permeable Sirt6 inhibitors.

Review 10. Biological and catalytic functions of sirtuin 6 as targets for small-molecule modulators.