Literature DB >> 27246220

Sirtuin 6 (SIRT6) Activity Assays.

Minna Rahnasto-Rilla1,2, Maija Lahtela-Kakkonen2, Ruin Moaddel3.   

Abstract

SIRT6 has been shown to possess weak deacetylation, mono-ADP-ribosyltransferase activity, and deacylation activity in vitro. SIRT6 selectively deacetylates H3K9Ac and H3K56Ac. Several SIRT6 assays have been developed including HPLC assays, fluorogenic assays, FRET, magnetic beads, in silico, and bioaffinity chromatography assays. Herein, we describe detailed protocols for the HPLC based activity/inhibition assays, magnetic beads deacetylation assays, bioaffinity chromatographic assays as well as fluorogenic and in silico assays.

Entities:  

Keywords:  Activation assays; Bioaffinity chromatography; Deacetylation assays; In silico screening; Inhibition assays

Mesh：

Substances：

Year:  2016        PMID： 27246220      PMCID： PMC5004593          DOI： 10.1007/978-1-4939-3667-0_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  Studying SIRT6 regulation using H3K56 based substrate and small molecules.

Authors:  Piia Kokkonen; Minna Rahnasto-Rilla; Paolo Mellini; Elina Jarho; Maija Lahtela-Kakkonen; Tarja Kokkola
Journal:  Eur J Pharm Sci       Date:  2014-07-06       Impact factor: 4.384

2.  Peptides and Pseudopeptides as SIRT6 Deacetylation Inhibitors.

Authors:  Piia Kokkonen; Minna Rahnasto-Rilla; Päivi H Kiviranta; Tero Huhtiniemi; Tuomo Laitinen; Antti Poso; Elina Jarho; Maija Lahtela-Kakkonen
Journal:  ACS Med Chem Lett       Date:  2012-10-23       Impact factor: 4.345

3.  Activation of the protein deacetylase SIRT6 by long-chain fatty acids and widespread deacylation by mammalian sirtuins.

Authors:  Jessica L Feldman; Josue Baeza; John M Denu
Journal:  J Biol Chem       Date:  2013-09-18       Impact factor: 5.157

4.  Hepatic-specific disruption of SIRT6 in mice results in fatty liver formation due to enhanced glycolysis and triglyceride synthesis.

Authors:  Hyun-Seok Kim; Cuiying Xiao; Rui-Hong Wang; Tyler Lahusen; Xiaoling Xu; Athanassios Vassilopoulos; Guelaguetza Vazquez-Ortiz; Won-Il Jeong; Ogyi Park; Sung Hwan Ki; Bin Gao; Chu-Xia Deng
Journal:  Cell Metab       Date:  2010-09-08       Impact factor: 27.287

5.  A fluorogenic assay for screening Sirt6 modulators.

Authors:  Jing Hu; Bin He; Shiva Bhargava; Hening Lin
Journal:  Org Biomol Chem       Date:  2013-08-28       Impact factor: 3.876

6.  Synthesis and characterization of SIRT6 protein coated magnetic beads: identification of a novel inhibitor of SIRT6 deacetylase from medicinal plant extracts.

Authors:  M Yasuda; D R Wilson; S D Fugmann; R Moaddel
Journal:  Anal Chem       Date:  2011-09-02       Impact factor: 6.986

7.  A FRET-based assay for screening SIRT6 modulators.

Authors:  Yan Li; Ling You; Wenfei Huang; Jie Liu; Hong Zhu; Bin He
Journal:  Eur J Med Chem       Date:  2015-04-09       Impact factor: 6.514

Review 8.  Mammalian sirtuins: biological insights and disease relevance.

Authors:  Marcia C Haigis; David A Sinclair
Journal:  Annu Rev Pathol       Date:  2010       Impact factor: 23.472

9.  Developmental defects and p53 hyperacetylation in Sir2 homolog (SIRT1)-deficient mice.

Authors:  Hwei-Ling Cheng; Raul Mostoslavsky; Shin'ichi Saito; John P Manis; Yansong Gu; Parin Patel; Roderick Bronson; Ettore Appella; Frederick W Alt; Katrin F Chua
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-05       Impact factor: 11.205

10.  Thiomyristoyl peptides as cell-permeable Sirt6 inhibitors.

Authors:  Bin He; Jing Hu; Xiaoyu Zhang; Hening Lin
Journal:  Org Biomol Chem       Date:  2014-10-14       Impact factor: 3.876
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  4 in total

Review 1.  Emerging roles of SIRT6 in human diseases and its modulators.

Authors:  Gang Liu; Haiying Chen; Hua Liu; Wenbo Zhang; Jia Zhou
Journal:  Med Res Rev       Date:  2020-12-16       Impact factor: 12.944

2.  The Identification of a SIRT6 Activator from Brown Algae Fucus distichus.

Authors:  Minna K Rahnasto-Rilla; Padraig McLoughlin; Tomasz Kulikowicz; Maire Doyle; Vilhelm A Bohr; Maija Lahtela-Kakkonen; Luigi Ferrucci; Maria Hayes; Ruin Moaddel
Journal:  Mar Drugs       Date:  2017-06-21       Impact factor: 5.118

3.  Natural polyphenols as sirtuin 6 modulators.

Authors:  Minna Rahnasto-Rilla; Jonna Tyni; Marjo Huovinen; Elina Jarho; Tomasz Kulikowicz; Sarangan Ravichandran; Vilhelm A Bohr; Luigi Ferrucci; Maija Lahtela-Kakkonen; Ruin Moaddel
Journal:  Sci Rep       Date:  2018-03-07       Impact factor: 4.379

Review 4.  Sirtuins and SIRT6 in Carcinogenesis and in Diet.

Authors:  Maria de Céu Teixeira; Elena Sanchez-Lopez; Marta Espina; Maria Luisa Garcia; Alessandra Durazzo; Massimo Lucarini; Ettore Novellino; Selma B Souto; Antonello Santini; Eliana B Souto
Journal:  Int J Mol Sci       Date:  2019-10-07       Impact factor: 5.923
  4 in total

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