Sabine Kling1, Susana Marcos. 1. Instituto de Óptica, Daza de Valdés, Consejo Superior de Investigaciones Cientificas, Madrid, Spain. sabine@io.cfmac.csic.es
Abstract
PURPOSE: To evaluate corneal deformation with varying intraocular pressure and the dependency of the biomechanical response on the corneal hydration state, modulated by the storage solutions or postmortem period. METHODS: Thirty fresh enucleated porcine eyes were used for in vitro whole eye globe inflation experiments. The eyes were separated into five groups and treated with different solutions: 20% dextran, 8% dextran, 0.125% riboflavin-20% dextran, Optisol-GS (Bausch & Lomb, Rochester, NY), and one control group of virgin (untreated) eyes. Intraocular pressure was increased (from 15 to 55 mm Hg) and decreased (to 15 mm Hg) in 5-mm Hg steps and Scheimpflug images were taken at each step. Measurements were repeated after 24 hours. Thickness and curvature changes were analyzed as a function of intraocular pressure. RESULTS: Corneal deformation differed across conditions and hydration states. Dehydration by any dextran solution increased the hysteresis after the inflation/deflation cycle (14.29 vs 22.07 to 41.75 μm), whereas overnight hydration did not lead to a significant difference. Compared to control corneas, corneas treated with Optisol-GS showed the most similar behavior. Corneas treated with 0.125% riboflavin-20% dextran deformed most (Δthickness(max) = 38.27 μm), indicating a softening of the corneal tissue compared to control corneas (23.18 μm) and corneas treated with 8% dextran (21.01 μm) and 20% dextran (29.07 μm). Dextran instillation decreased corneal thickness on average to 56.5% at 0 hours and 72.7% at 24 hours. CONCLUSIONS: Corneal hydration and tissue preservation changed corneal biomechanics, in particular its relaxation over a period of 24 hours. Copyright 2013, SLACK Incorporated.
PURPOSE: To evaluate corneal deformation with varying intraocular pressure and the dependency of the biomechanical response on the corneal hydration state, modulated by the storage solutions or postmortem period. METHODS: Thirty fresh enucleated porcine eyes were used for in vitro whole eye globe inflation experiments. The eyes were separated into five groups and treated with different solutions: 20% dextran, 8% dextran, 0.125% riboflavin-20% dextran, Optisol-GS (Bausch & Lomb, Rochester, NY), and one control group of virgin (untreated) eyes. Intraocular pressure was increased (from 15 to 55 mm Hg) and decreased (to 15 mm Hg) in 5-mm Hg steps and Scheimpflug images were taken at each step. Measurements were repeated after 24 hours. Thickness and curvature changes were analyzed as a function of intraocular pressure. RESULTS: Corneal deformation differed across conditions and hydration states. Dehydration by any dextran solution increased the hysteresis after the inflation/deflation cycle (14.29 vs 22.07 to 41.75 μm), whereas overnight hydration did not lead to a significant difference. Compared to control corneas, corneas treated with Optisol-GS showed the most similar behavior. Corneas treated with 0.125% riboflavin-20% dextran deformed most (Δthickness(max) = 38.27 μm), indicating a softening of the corneal tissue compared to control corneas (23.18 μm) and corneas treated with 8% dextran (21.01 μm) and 20% dextran (29.07 μm). Dextran instillation decreased corneal thickness on average to 56.5% at 0 hours and 72.7% at 24 hours. CONCLUSIONS: Corneal hydration and tissue preservation changed corneal biomechanics, in particular its relaxation over a period of 24 hours. Copyright 2013, SLACK Incorporated.
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