Brecken J Blackburn1, Shi Gu1, Matthew R Ford2, Vinícius de Stefano2, Michael W Jenkins1,3, William J Dupps1,2,4, Andrew M Rollins1. 1. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, United States. 2. Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, United States. 3. Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio, United States. 4. Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States.
Abstract
Purpose: There is strong evidence that abnormalities in corneal biomechanical play a causal role in corneal ectasias, such as keratoconus. Additionally, corneal crosslinking (CXL) treatment, which halts progression of keratoconus, directly appeals to corneal biomechanics. However, existing methods of corneal biomechanical assessment have various drawbacks: dependence on IOP, long acquisition times, or limited resolution. Here, we present a method that may avoid these limitations by using optical coherence tomography (OCT) to detect the endogenous random motion within the cornea, which can be associated with stromal crosslinking. Methods: Phase-decorrelation OCT (PhD-OCT), based in the theory of dynamic light scattering, is a method to spatially resolve endogenous random motion by calculating the decorrelation rate, Γ, of the temporally evolving complex-valued OCT signal. PhD-OCT images of ex vivo porcine globes were recorded during CXL and control protocols. In addition, human patients were imaged with PhD-OCT using a clinical OCT system. Results: In both the porcine cornea and the human cornea, crosslinking results in a reduction of Γ (P < 0.0001), indicating more crosslinks. This effect was repeatable in ex vivo porcine corneas (change in average Γ = -41.55 ± 9.64%, n = 5) and not seen after sham treatments (change in average Γ = 2.83 ± 12.56%, n = 5). No dependence of PhD-OCT on IOP was found, and correctable effects were caused by variations in signal-to-noise ratio, hydration, and motion. Conclusions: PhD-OCT may be a useful and readily translatable tool for investigating biomechanical properties of the cornea and for enhancing the diagnosis and treatment of patients.
Purpose: There is strong evidence that abnormalities in corneal biomechanical play a causal role in corneal ectasias, such as keratoconus. Additionally, corneal crosslinking (CXL) treatment, which halts progression of keratoconus, directly appeals to corneal biomechanics. However, existing methods of corneal biomechanical assessment have various drawbacks: dependence on IOP, long acquisition times, or limited resolution. Here, we present a method that may avoid these limitations by using optical coherence tomography (OCT) to detect the endogenous random motion within the cornea, which can be associated with stromal crosslinking. Methods: Phase-decorrelation OCT (PhD-OCT), based in the theory of dynamic light scattering, is a method to spatially resolve endogenous random motion by calculating the decorrelation rate, Γ, of the temporally evolving complex-valued OCT signal. PhD-OCT images of ex vivo porcine globes were recorded during CXL and control protocols. In addition, humanpatients were imaged with PhD-OCT using a clinical OCT system. Results: In both the porcine cornea and the human cornea, crosslinking results in a reduction of Γ (P < 0.0001), indicating more crosslinks. This effect was repeatable in ex vivo porcine corneas (change in average Γ = -41.55 ± 9.64%, n = 5) and not seen after sham treatments (change in average Γ = 2.83 ± 12.56%, n = 5). No dependence of PhD-OCT on IOP was found, and correctable effects were caused by variations in signal-to-noise ratio, hydration, and motion. Conclusions: PhD-OCT may be a useful and readily translatable tool for investigating biomechanical properties of the cornea and for enhancing the diagnosis and treatment of patients.
Authors: Tukezban Huseynova; George O Waring; Cynthia Roberts; Ronald R Krueger; Minoru Tomita Journal: Am J Ophthalmol Date: 2014-01-02 Impact factor: 5.258
Authors: Cheng W Hong; Abhijit Sinha-Roy; Lynn Schoenfield; James T McMahon; William J Dupps Journal: Invest Ophthalmol Vis Sci Date: 2012-04-30 Impact factor: 4.799
Authors: Ravi Kiran Manapuram; Salavat R Aglyamov; Floredes M Monediado; Maleeha Mashiatulla; Jiasong Li; Stanislav Y Emelianov; Kirill V Larin Journal: J Biomed Opt Date: 2012-10 Impact factor: 3.170
Authors: Keyton Clayson; Thomas Sandwisch; Yanhui Ma; Elias Pavlatos; Xueliang Pan; Jun Liu Journal: Curr Eye Res Date: 2019-09-18 Impact factor: 2.424
Authors: Rafael Grytz; Kapil Krishnan; Ryan Whitley; Vincent Libertiaux; Ian A Sigal; Christopher A Girkin; J Crawford Downs Journal: Comput Methods Appl Mech Eng Date: 2019-10-01 Impact factor: 6.756
Authors: Tanner J Ferguson; Srinidhi Singuri; Sanjai Jalaj; Matthew R Ford; Vinicius S De Stefano; Ibrahim Seven; William J Dupps Journal: Transl Vis Sci Technol Date: 2021-04-29 Impact factor: 3.283