Important developments in romanian propolis research.

The most important developments in propolis analysis and pharmacological properties are discussed. In order to help in the Romanian propolis standardization, different methodologies for chemical composition analysis (UV-VIS, HP-TLC, and HPLC-DAD) are reviewed using new approaches and software (fuzzy divisive hierarchical clustering approach and ChromQuest software) and compared with international studies made until now in propolis research. Practical applications of Romanian propolis in medicinal therapy and cosmetics are reviewed, and quality criteria for further standardization are proposed.

1. Introduction

Propolis is a natural material, sticky and resinous, collected by bees from different tree buds and exudates found on plant wounds, mixed with own substances like enzymes, and transformed in order to be used for sealing different holes in the hive, to clean the comb cells or to hygienize the entire hive [1-3]. Bees produce propolis as a means of defense against microbes, moulds, and for “embalming” the intruders that may enter into the hive, preventing their putrefaction [1, 4].

Beside its uses to seal and clean the hive, propolis may be called a “chemical weapon” of the bees against human pathogen microorganisms and viruses [5-13], as well as bee pathogens [14-17].

Due to the popularity of using propolis in medicine and other domains, thousands of studies were developed all over the world, studying the chemical composition, functions, or different properties of propolis extracts. More than 2700 articles and 250 books were published in Elsevier journals or publishing house (http://www.elsevier.com/), more than 2000 articles in Medline (http://www.ncbi.nih.gov/), and more than 1500 studies in Wiley-Blackwell database (http://www.onlinelibrary.wiley.com/).

Most of the current literature concerning propolis is focused on determining the chemical composition and biological activity of propolis, beside determining the botanical and geographical origins, by analyzing comparatively the resins from which propolis is derived [4, 6, 18–20], but less studies have examined the antimicrobial properties of propolis against bee pathogens or honey bee immune responses [14, 17, 21, 22].

The present study aimed to review the publications upon Romanian propolis with respect to composition, bioavailability, and biological properties.

2. Propolis Origin

Scientific studies demonstrate that propolis components came from three distinctive sources:

vegetal: plant exudates collected by the bees resins secreted by the buds of poplar, pine, birch, chestnut, maple [3, 18, 23, 24], and lipophilic substances secreted by plant wounds, resins, or gums [25-29];

animal: substances secreted by the bees (wax, saliva) [3, 29, 30];

incidental materials introduced during propolis production (pollen, nectar or honey) [3, 30–33].

Depending on the plant source collected by the bees, propolis color may vary from golden yellow, to red, green, or dark brown.

The complex chemical composition of propolis is due to the plant material used as the raw material by the bees, reason also for the wide range of compounds (in number and quantity) that are found in propolis from different parts of the world. Scientific studies on the chemical composition of propolis and different tree or other plant species from the surroundings of the collection places of propolis samples indicate the relation between the plant material and propolis samples from the same region: Populus nigra, Populus italic, and  Populus tremula (Bulgaria and Mongolia) [34, 35], Betula, Populus, Pinus, Acacia, and Aesculus (Hungary) [36], Betula, Pinus, and Salix (Poland) [37], deciduous trees and meadows (Lithuania) [38], Populus and Betula (Russia) [39], Populus trichocarpa and Populus tremuloides (Canada) [40], Clusia minor and Clusia major (Venezuela) [41-43], and Baccharis spp. (Brazil) [25, 44, 45].

Studies on chemical composition of propolis from different geographical origins show that characteristic classes of compounds for different geographical origins also correlated with plant source [10, 18, 46]. Birtaş-Gagea [46] made a detailed study for footprinting different Romanian plant sources extracts (poplar, birch, willow, and pine buds) compared with propolis extract, revealing that secondary metabolites from the mentioned plant sources, especially poplar, are found also in Romanian propolis samples.

3. Early Researches in Romanian Propolis

As most of the propolis samples from Central and Eastern Europe, Romanian propolis plant sources are resins secreted by buds of Populus nigra, Quercus, Aesculus hippocastanum, Ulmus, Picea, and Fraxinus. Romanian poplar buds have been analyzed [47] for their flavonoid content using thin layer chromatography, and comparative studies were made with propolis extracts [48]. This showed that both contained the same pattern of flavonoids: chrysin, tectochrysin, pinocembrin, galangin, kaempferol, apigenin, and quercetol. For most of the compounds, the levels found in propolis were slightly higher than in poplar buds. The total flavonoid content was slightly higher in the poplar buds extract, due to the presence of beeswax, pollen, and other impurities in propolis. Methanolic extracts of Populus nigra, Betula pendula, Salix alba, and Pinus were analyzed [49], showing that these plant sources were used by the bees for propolis production.

Many research groups have found evidence of biological activities of Romanian propolis extract. Early studies were focused on the effects of certain blood constituents and lymphatic system [50, 51], hepatotoxic and hepatoprotector effects [52, 53], and antibacterial, antiviral, and anti-inflammatory effect [54-59]. Many studies were made on the action of a standardized propolis extract (SPE) on rat liver [60-62].

Propolis extracts were also investigated for their action in agriculture. Different studies were focused on the germinative effect in different plant seeds [63-65] and vine [66]. Different water extracts of propolis were used as germination substrates and the stimulatory effect upon germination was established [67]. Onion (Allium cepa L.), linseed (Linum usitatissimum L.), wheat (Triticum sp.), and oat (Avena sativa) were monitored during 24 and 112 hours in substrates containing different dilutions of propolis aqueous solution, establishing daily germination percentages. Seeds of elegant zinnia (Zinnia elegance), Mexican marigold (Tagetes erecta), pot marigold (Calendula officinalis), strawflower (Helichrysum bracteatum), and marvel of Peru (Mirabilis jalapa) were stimulated in their germination capacity by 0.5% aqueous solution of propolis, compared with control groups. Vine layers for shoot formation and root striking, into water solutions of propolis in different concentrations, were followed for their time bill and bud-breaking, moment of advent of shoots, speed of growth, and average number of roots/layers. Low concentrations of propolis solutions (0.1%; 0.05% and 0.01%) present a stimulation of shoot formation and rooting.

In vitro studies of propolis extracts on different plant tissue micropropagation (carnation, onion) were performed, and interesting results were obtained [68-72]. Beside the stimulation of growth for in vitro cultivation of the plants, using propolis in the cultivation media, no infection appears in the culture vessels, with propolis playing a double role of stimulant and antiseptic.

4. Chemical Composition

Propolis is one of the most studied Romanian bee products. For chemical composition analysis, best extraction procedures were developed and comparatively assessed  [49, 73–75]. Solid-liquid extraction with different ethanol concentrations was performed in reflux condenser for one hour at 65°C or at room temperature for different periods of time (4–6 days) [49, 76] and compared with water extracts of propolis with respect to biologically active compounds extracted [77]. Detailed reports and Ph.D. degree theses on chemical composition [46, 78–86] and plant origin from bud exudates of Populus spp. and other species [47–49, 84, 87] were made and the common conclusion was that Romanian propolis possessed high amounts of biologically active compounds from the classes of phenolic acids and different classes of flavonoids (flavones/flavonols, flavanones/dihydroflavonols, or other phenols) [16, 46, 49, 85] and can be subjected to other “validated” methods for European poplar type propolis [88].

A simple method for total flavonoid determination was developed by Tămaş [89]. Different complementary colorimetric methods are required for total flavonoid determination, as aluminium chloride reacts only with flavone/flavonols and 2,4-dinitriphenylhydrazine reacts with flavanones/flavanonols [90]. This simple method suggests zirconium reagent for total flavonoid determination. The method is simple, reliable, and fast requiring only one reagent which reacts with all specific flavonoid compounds, estimating their total content. The method was used and verified by different research groups in Romania [84, 85].

For the first time a study on Romanian propolis analysis was made using reflectance spectroscopy and chemometric treatment of digitized spectra of solid propolis samples [91] and demonstrated that the raw samples of propolis carry valuable information about their origin analyzing the color, physical, and other chemical constituents. Cluster analysis (CA), principal component analysis (PCA), and linear discriminant analysis (LDA) were successfully applied to spectroscopic and score matrices. Having different botanical origin, propolis exhibits different colors (light orange, dark with reddish tint, dark with green tint etc.) and different UV-spectra. Based on the difference in color. A methodology of sampling technique and data analysis using chemometric methods applied on reflectance UV-spectra analysis of solid samples, was developed for the first time, based only on the difference in color, without any sample extraction, solvent use or sophisticated equipment needed.

Another simple method in discrimination and authentication of propolis samples is based on fuzzy clustering of thin layer chromatographic data via image analysis [86]. Known concentrations of propolis ethanolic extracts were applied on HP-TLC silica gel 60 precoated plates and eluted with toluene-ethyl acetate-formic acid (30 : 12 : 5) and developed with 0.2% diphenylboryloxyethylamine and 4% polyethyleneglycol. The fluorescent images were processed using TLC Analyzer software [92]. The results are in agreement with botanical origin and vegetation zone and conduct to the identification of two types of propolis: meadow area and forest area, with three subgroups in the first type and two subgroups in the second type.

As mentioned before, propolis has a very complex chemical composition, depending on the flora from areas where it is collected. Romanian propolis proved to belong to the temperate zone propolis regarding the chemical composition.

The research group of Coneac et al. [76] analyzing propolis from west side of Romania by HPLC-DAD identified and quantified caffeic acid, rutin, quercetin, apigenin, and chrysin, both in “hot” (reflux extraction) and “cold” extracts (maceration in different concentrations of ethanol). Using 20%, 60%, and 96% ethanol, different results were obtained: higher concentration of ethanol extracting more hydrophobic bioactive compounds (apigenin, kaempferol, and chrysin), compared with hydrophilic ones (caffeic acid and quercetin).

The study of Mihai [85], investigating 53 propolis samples from Transylvania by means of HPLC-DAD, identified and quantified 10 compounds (phenolic acids and flavonoids) using external standard method. Calibration curves for each standard were made for exact quantification of compounds from propolis extracts. The identified compounds were syringic acid, caffeic acid, p-coumaric acid, ferulic acid, t-cinnamic acid, vanillin, pinocembrin, chrysin, galangin, and pinostrobin (Figure 1). All analyzed samples contain phenolic acids caffeic, p-coumaric, and ferulic and also chrysin. Most of the samples contain pinocembrin and galangin. Chrysin is the reference flavonoid in poplar propolis and was quantified in higher amounts in Transylvanian samples (1.6 mg/g propolis). The conclusion of the study was that also Transylvanian propolis belonged, to poplar type and using chrysin, galangin or pinocembrin in spectrophotometric determination of total flavonoids from propolis is correct, those compounds being present in almost all analyzed samples.

Figure 1

HPLC chromatogram of a Romanian propolis extract and the identified compounds/retention time [85].

5. Antioxidant Activity

The antioxidant activity of Romanian propolis was studied and high biological activity was correlated with phenolic fraction of the extracts [76, 93–97].

Coneac et al. [76] suggest that using the DPPH method for radical scavenging activity measurement, relative absorbance A% must be calculated as the ratio between the absorbance at time t and initial absorbance, and the A% values used for comparis would be those from t time when A% became constant. Generally phenolic content is correlated with antioxidant activity, as the flavonoids are the major compounds responsible for this activity [95, 96].

An interesting work investigates the intimate molecular-level mechanisms of propolis extract, known for its antioxidant activity [93]. Electron paramagnetic resonance (EPR) detectable free radical signals are described for the first time in propolis extracts. The shape of these signals and the conditions in which they were obtained point to polyphenolic flavonoids as the sites of the radicals. An inverse correlation between antioxidant capacity and free radical signal intensity is shown. The free radical reactivity of propolis is also investigated by the effect that it exerts on the biologically relevant peroxide reactivity of hemoglobin. With this study, a new test of antioxidant activity from natural extracts is proposed, based on modulation of the ascorbate peroxidase activity of hemoglobin (HAPX). Obtained results correlate with those obtained by traditional methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), or on 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)).

An effective measure of antioxidant capacity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) bleaching kinetic profiles has been developed using principal component analysis (PCA) [97]. The activity score as well as a related parameter, called quercetin factor (QF), was used to estimate antioxidant capacity of different propolis extracts based on the first principal component (which explains 98% of the total variance). Determination of the QF parameter requires less time and reagents than previous DPPH-based antioxidant capacity parameters but does require additional equipment. Additionally, UV-VIS and FT-IR spectroscopic analyses of propolis extracts have been performed and correlated to antioxidant capacity, to offer a spectroscopic and reagent-less rapid evaluation method of the antioxidant activity of biological samples. This test battery may be an interesting tool for antioxidant capacity, floral origin, and geographic location of propolis and other bee products.

6. Biological Properties

Antibacterial, antifungal, or antiviral activities were studied on propolis extracts of different geographical origin from Romania [16, 54–59, 98–103].

Three Gram-positive bacteria (Staphylococcus aureus ATCC 6538P, Bacillus cereus ATCC 14579, and Listeria monocytogenes ATCC 7644), two Gram-negative bacteria (Escherichia coli ATCC 10536 and Pseudomonas aeruginosa ATCC 27853), and one yeast strain (Candida albicans ATCC 90028) were used for testing the antibacterial activity of Romanian propolis [101]. Gram-positive bacteria were more sensitive to propolis extract, using inhibition zone method, while Gram-negative bacteria were more resistant. Pseudomonas aeruginosa was not inhibited by 5% ethanolic propolis solution. This bacterial strain is known for its resistance towards antibiotics also. Similar findings were obtained by Stan et al. [102] on Pseudomonas aeruginosa and Staphylococcus intermedius.

Propolis samples from different geographical origin (different botanical origin) exhibit different antibacterial activity due to the interactions between the biologically active compounds present in the composition. The most recent study on Romanian propolis and its antibacterial activity on Paenibacillus larvae, the bacterial pathogen that causes American Foulbrood, a larval disease that can kill the honeybee colony, show very interesting results [16]. Different propolis extracts from Transilvania region (Romania) show significantly inhibition process of P. larvae tested in vitro. The extracts showed major differences in the content of total flavonoids (ranging from 2.4% to 16.4%) and the total polyphenols (ranging between 23.3% and 63.2%). The study revealed that it is not only the content of compounds in propolis which influences the strength of antimicrobial effects, but there is also a significant interaction effect among flavonoids of the propolis extracts. With this study, it was proposed that interaction effects among the various chemical compounds in propolis should be taken into account when considering the antibacterial effects against honeybee pathogens.

Romanian propolis was also used in different pharmaceutical and cosmetic formulations [77, 104–107]. A study made with propolis and lycopene extract nanoemulsion preparations confers better therapeutic effects than those of the conventional formulations, based on local control release of dozed form, for a longer period of time, which probably improves its efficiency and skin acceptance, meaning a better compliance [77]. This study is very interesting and useful for different cosmetic utilization of these nanoemulsions in skin's protective mechanisms against UVA radiation. The information obtained in the study of Butnariu and Giuchici [77] suggests that administration of propolis and lycopene aqueous extract nanoemulsion is safe and can be also useful for preclinical studies because of the high potential both regarding its efficiency (the analgesic effect) and therapeutic safety.

Propolis extracts previously characterized by HPLC were used to obtain micro/nanoparticles by encapsulation in β-cyclodextrin (solution method), and the complexes were analyzed again in order to evaluate the morphology and the dimensions of crystals and to evaluate the encapsulated biocompounds [104]. It was interesting to observe that the nanoparticles containing propolis extracts release the biologically active compounds more gradually, compared to propolis extract.

7. Quality Criteria for Propolis Standardization

In order to be accepted into the healthcare system, propolis needs chemical analysis performed by some standardized methods that may be applied all over the world. From this statement came the real problem for the researchers, because what can be observed from the existing studies is that propolis is very complex and different from one geographical region to another. This issue has an answer that propolis may be standardized, if different propolis types are formulated by their plant source and corresponding chemical profile. If regional standardization is taken into account, for poplar type propolis, which is the most studied and the best known propolis type, a battery of tests can be made to standardize the main compounds.

Stan et al. [108] made a description of compositional characteristics for 56 Romanian propolis samples, proposing a battery of tests for future standardization. For covering all quality aspects, further work on more samples from all locations in Romania is needed in the future. Once a pattern of chemical constituents is well established (with high number of samples from all geographical regions in Romania and analyzed by laboratory validated methods), a standard for propolis analysis will be suitable for debating at national level. The most often techniques used generally for chemical analysis of propolis are spectrophotometric methods [23, 76, 80, 83, 84, 88, 90, 108], high performance liquid chromatography (HPLC) coupled with different detectors [10, 26, 27, 45, 49, 76, 78, 85, 109–112], and gas chromatography-mass spectrometry (GC-MS) [87, 112–115]. Interesting results were obtained [49] (Figure 2) in a simple spectrophotometric registration of UV-Visible spectra of different propolis extracts, correlated with total flavonoid content, where 3 types of propolis may be distinguished.

Figure 2

Overlapped UV-VIS spectra of different propolis extracts: I—propolis extract having λ max⁡ = 320 nm and total flavonoid content < 2%; II—propolis extract having a plateau as UV maxima between 320 and 395 nm and total flavonoid content approximately of 5%; III—propolis extract having λ max⁡ = 295 nm and total flavonoid content of 5%–8% [49].

Propolis spectra with λ max⁡ = 320 nm have total flavonoid content < 2%, and radical scavenging activity situated between 8% and 14%.

Propolis spectra having a plateau as UV maxima between 320 and 395 nm exhibit a total flavonoid content approximately of 5% and radical scavenging activity of 14%–18%.

Propolis spectra having λ max⁡ = 295 nm show total flavonoid content of 5%–8% and radical scavenging activity of more than 18%.

For quality purposes, propolis sampling procedures must include the type of collection method, because this may influence the quality of final product (tincture) [116]. Quality standards for bee products in general and propolis in particular must be for the benefit of producers, distributors, and consumers as well as. The present study shows that higher content of wax was determined in propolis samples obtained by scrapping the frames with hive tools than in samples obtained through propolis collector. Higher amounts of biologically active compounds were obtained from samples collected through propolis collector.

As a health promoting product, propolis quality must be always very high, even if there is still no certified system of quality control available worldwide [108]. Nevertheless, good apicultural practices should be applied by the beekeepers first and then the producers of different propolis-based products.

8. Conclusion

Propolis is a natural product with high potential for use in human consumption and medicinal uses. Quality control and chemical composition reveal that, generally, Romanian propolis is a high quality product, suitable for human consumption and uses in medicinal formulations. A high number of samples covering most of Romanian territory are analyzed with standardized methods available at this moment and a battery of tests for future standardization is proposed at this moment. The complex chemical composition of propolis requires further research in order to explain better its biological activities on different pathogens. Colony collapse disorder affects the bees all over the world, and self-medication of the bees is now of great interest. Initiated studies on using propolis for this purpose are in attention of different research groups from Romania, and original studies will be published in the near future.