BACKGROUND: There have been very few detailed reports of the intestinal environment after surgical treatment for colorectal cancer (CRC). We analysed faecal microbiota, organic acids and pH to investigate the influence of colorectal surgery on the intestinal environment. METHODS: Faecal samples from 81 CRC patients were collected before the start of pre-operative preparation the day before surgery, as well as 7 days or more after surgery. Thirteen groups of intestinal microbiota, eight types of organic acids, and pH were measured using 16S rRNA-targeted reverse transcription-quantitative PCR, high-performance liquid chromatography and a pH meter, respectively. RESULTS: Total bacterial counts (10.3 ± 0.6 vs. 9.4 ± 1.2 log10 cells/g; p < 0.001) and the numbers of 6 groups of obligate anaerobes were significantly decreased after surgery. In contrast, the populations of Enterobacteriaceae, Enterococcus, Staphylococcus and Pseudomonas were significantly increased. Post-operatively, the concentration of total organic acids was lower (77.9 ± 40.1 vs. 50.1 ± 37.0 μmol/g; p < 0.001) than the pre-operative concentration, and a significant reduction in short-chain fatty acids (SCFAs) was observed. CONCLUSION: Significant changes in the intestinal environment, including marked decreases in obligate anaerobes, increases in pathogenic bacteria, and reductions in SCFAs, were detected after surgery for CRC.
BACKGROUND: There have been very few detailed reports of the intestinal environment after surgical treatment for colorectal cancer (CRC). We analysed faecal microbiota, organic acids and pH to investigate the influence of colorectal surgery on the intestinal environment. METHODS: Faecal samples from 81 CRC patients were collected before the start of pre-operative preparation the day before surgery, as well as 7 days or more after surgery. Thirteen groups of intestinal microbiota, eight types of organic acids, and pH were measured using 16S rRNA-targeted reverse transcription-quantitative PCR, high-performance liquid chromatography and a pH meter, respectively. RESULTS: Total bacterial counts (10.3 ± 0.6 vs. 9.4 ± 1.2 log10 cells/g; p < 0.001) and the numbers of 6 groups of obligate anaerobes were significantly decreased after surgery. In contrast, the populations of Enterobacteriaceae, Enterococcus, Staphylococcus and Pseudomonas were significantly increased. Post-operatively, the concentration of total organic acids was lower (77.9 ± 40.1 vs. 50.1 ± 37.0 μmol/g; p < 0.001) than the pre-operative concentration, and a significant reduction in short-chain fatty acids (SCFAs) was observed. CONCLUSION: Significant changes in the intestinal environment, including marked decreases in obligate anaerobes, increases in pathogenic bacteria, and reductions in SCFAs, were detected after surgery for CRC.
Authors: Peter J Turnbaugh; Ruth E Ley; Michael A Mahowald; Vincent Magrini; Elaine R Mardis; Jeffrey I Gordon Journal: Nature Date: 2006-12-21 Impact factor: 49.962
Authors: Steven R Gill; Mihai Pop; Robert T Deboy; Paul B Eckburg; Peter J Turnbaugh; Buck S Samuel; Jeffrey I Gordon; David A Relman; Claire M Fraser-Liggett; Karen E Nelson Journal: Science Date: 2006-06-02 Impact factor: 47.728
Authors: Rajesh Pendlimari; Robert R Cima; Bruce G Wolff; John H Pemberton; Marianne Huebner Journal: J Am Coll Surg Date: 2012-02-08 Impact factor: 6.113
Authors: Marc Gh Besselink; Hjalmar C van Santvoort; Erik Buskens; Marja A Boermeester; Harry van Goor; Harro M Timmerman; Vincent B Nieuwenhuijs; Thomas L Bollen; Bert van Ramshorst; Ben Jm Witteman; Camiel Rosman; Rutger J Ploeg; Menno A Brink; Alexander Fm Schaapherder; Cornelis Hc Dejong; Peter J Wahab; Cees Jhm van Laarhoven; Erwin van der Harst; Casper Hj van Eijck; Miguel A Cuesta; Louis Ma Akkermans; Hein G Gooszen Journal: Lancet Date: 2008-02-14 Impact factor: 79.321
Authors: Carlos E Pineda; Andrew A Shelton; Tina Hernandez-Boussard; John M Morton; Mark L Welton Journal: J Gastrointest Surg Date: 2008-07-12 Impact factor: 3.452
Authors: M Wiegerinck; S K Hyoju; J Mao; A Zaborin; C Adriaansens; E Salzman; N H Hyman; O Zaborina; H van Goor; J C Alverdy Journal: Br J Surg Date: 2018-04-16 Impact factor: 6.939
Authors: Yan-Jie Hu; Ka Li; Li Li; Xiao-Dong Wang; Jie Yang; Jin-Hua Feng; Wei Zhang; Yu-Wei Liu Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2017-01-20
Authors: Gabor S A Abis; Hein B A C Stockmann; Marjolein van Egmond; Hendrik J Bonjer; Christina M J E Vandenbroucke-Grauls; Steven J Oosterling Journal: J Gastrointest Surg Date: 2013-10-11 Impact factor: 3.452
Authors: L Mira-Pascual; R Cabrera-Rubio; S Ocon; P Costales; A Parra; A Suarez; F Moris; L Rodrigo; A Mira; M C Collado Journal: J Gastroenterol Date: 2014-05-09 Impact factor: 7.527