Open access to tree genomes: the path to a better forest.

An open-access culture and a well-developed comparative-genomics infrastructure must be developed in forest trees to derive the full potential of genome sequencing in this diverse group of plants that are the dominant species in much of the earth's terrestrial ecosystems.

Opportunities and challenges in forest tree genomics are seemingly as diverse and as large as the trees themselves; however, here, we have chosen to focus on the potential significant impact on all of tree biology research if only an open-access culture and comparative-genomics infrastructure were developed. In earlier articles [1,2], we argued that the great diversity of forest trees found in both the undomesticated and domesticated state provides an excellent opportunity to understand the molecular basis of adaptation in plants and furthermore that comparative-genomic approaches will greatly facilitate discovery and understanding. We identified several priority research areas towards realizing these goals (Box 1), such as establishing reference genome sequences for important tree species, determining how to apply sequencing technologies to understand adaptation, and developing resources for storing and accessing forestry data. Significant progress has been made in many of these priorities, with the exception of investments in database resources and understanding ecological functions. Here, we briefly summarize the rapid progress in developing genomic resources in a small number of species and then offer our view on what we believe it will take to realize the final two priorities.

The great diversity found in forest trees

There are an estimated 60,000 tree species on earth, and approximately 30 of the 49 plant orders contain tree species. Clearly, the tree phenotype has evolved many times in plants. The diversity of plant structures, development, life history, environments occupied and so on in trees is nearly as broad as higher plants in general, but trees share the common characteristic that all are perennial and many are very long lived. Because of the sessile nature of plants, each tree must survive and reproduce in a specific environment over the seasonal cycles of its lifetime. This tight association between individual genotypes and their environment provides a powerful research setting, just as it has driven the evolution of a plethora of uniquely arboreal adaptations. Understanding these evolutionary strategies is a long-standing area of study of tree biologists, with many broader biological implications.

Completed and current genome-sequencing projects in forest trees are limited to about 25 species from just 4 of more than 100 families: Pinaceae (pines, spruces and firs), Salicaceae (poplars and willows), Myrtaceae (eucalyptus) and Fagaceae (oaks, chestnuts and beeches). Large-scale sequencing projects such as the 1000 Human Genomes [3], 1000 Plant Genomes (1KP) [4] or the 5000 Insect Genome (i5k) [5] projects have not yet been proposed for forest trees.

Rapidly developing genomic resources in forest trees

Genome resources are developing rapidly in forest trees in spite of the challenges associated with working with large, long-lived organisms and sometimes very large genomes [2]. Complete genome sequencing, however, has been slow to advance in forest trees owing to funding limitations and the large size of conifer genomes. Black cottonwood (Populus trichocarpa Torr. & Gray) was the first forest tree genome to be sequenced by the US Department of Energy Joint Genome Institute (DOE/JGI) [6] (Table 1). Black cottonwood has a relatively small genome (450 Mb) and is a target feedstock species for cellulosic ethanol production, and thus fits into the DOE/JGI priority of sequencing bioenergy feedstock species. The genus Populus has 30+ species (aspens and cottonwoods) with genome sizes of approximately 500 Mb. Several species are being sequenced by DOE/JGI, and other groups around the world, and it seems likely that all members of the genus will soon have a genome sequence (Table 1). The next forest tree to be sequenced was the flooded gum (Eucalyptus grandis BRASUZ1, which is a member of the Myrtaceae family), again by DOE/JGI. Eucalyptus species and their hybrids are important commercial species grown in their native Australia and many regions throughout the southern hemisphere. Several more eucalyptus species are being sequenced (Table 1), each with relatively small genomes (500 Mb), but it will probably take many years before all 700+ members of this genus are completed. Several members of the Fagaceae family are now being sequenced (Table 1). Members of this group include the oaks, beeches and chestnuts, with genome sizes less than 1 Gb.

Table 1

Genome resources in forest trees

Family	Genus	Species	Sequenceaccess	Genome	Related publications
Pinaceae	Pinus	taeda (loblolly pine)	[14,19]	Resequenced amplicons
			[20]	BACs	[21,22]
			[14,19]	Fosmids
			[23]	Draft genome complete
	Pinus	lambertiana (sugar pine)	[14,19]	Resequenced amplicons	[24]
			[23]	Draft genome (in progress)
	Pseudotsuga	menziesii (Douglas-fir)	[14,19]	Resequenced amplicons	[25]
			[23]	Draft genome (in progress)
	Pinus	sylvestris (Scots pine)	[26]	Draft genome (in progress)
	Pinus	pinsater (maritime pine)	[26]	Draft genome (in progress)
			[14,19]	Resequenced amplicons	[27]
	Pinus	sibirica (Siberian pine)	[28]	Draft genome (in progress)
	Pinus	radiata (Monterey pine)		Draft genome (in progress)
			[14,19]	Resequenced amplicons	[29]
	Picea	abies (Norway spruce)	[14,19]	Resequenced amplicons	[30]
			[31]	Draft genome complete (restricted)
	Picea	glauca (white spruce)	[32]	Draft genome complete
			[14,19]	BACs	[33]
	Larix	sibirica (Siberian larch)	[28]	Draft genome (in progress)
Salicaceae	Populus	trichocarpa (black cottonwood)	[34]	Genome complete	[6]
			[19]	BACs	[35]
				Genome resequencing (restricted)	[36]
	Populus	tremula (European aspen)	[37]	Draft genome complete
	Populus	tremula x tremuloides	[37]	Draft genome complete
	Populus	tremuloides (quaking aspen)	[37]	Draft genome complete
			[19]	BACs	[38]
	Populus	grandidentata (bigtooth aspen)	[37]	Draft genome complete
	Populus	nigra (black poplar)	[39]	Draft genome complete (restricted)
	Salix	purpurea (purpleosier willow)	[40]	Draft genome complete (restricted)
Myrtaceae	Eucalytpus	grandis (rose gum)	[19]	BACs	[41]
			[34]	Draft genome complete
	Eucalyptus	globulus (blue gum)	[42]	Draft genome (in progress)
	Eucalyptus	camaldulensis (river red gum)	[43]	Draft genome complete	[44]
	Corymbia	citriodora (lemon-scented gum)	[45]	Draft genome complete (restricted)
Fagaceae	Quercus	robur (English oak)	[19]	BACs	[46,47]
			[48]	Draft genome (in progress)
	Castanea	mollissima (Chinese chestnut)	[49]	Draft genome (in progress)
			[50]	BACs	[51]
Betulaceae	Betula	nana (dwarf birch)	[52]	Draft genome complete	[53]
Oleaceae	Fraxinus	excelsior (European ash)	[54]	Draft genome complete

Details current genome sequencing projects in forest trees with sequence access information and relevant publications.

The gymnosperm forest trees (such as the conifers) were the last to enter the world of genome sequencing. This was entirely due to their very large genomes (10 Gb and greater) as they are extremely important economically and ecologically, and phylogenetically they represent the ancient sister lineage to that of angiosperm species. Genome resources needed to support a sequencing project were reasonably well developed, but it was not until the introduction of next-generation sequencing (NGS) technologies that sequencing conifer genomes became tractable. Currently, there are at least ten conifer (Pinaceae) genome-sequencing projects under way (Table 1).

Aside from reference genome sequencing in forest trees, there is significant activity in transcriptome sequencing and resequencing for polymorphism discovery (Tables 2 and 3). We have only listed the transcriptome and resequencing projects in Table 1 that are associated with a species that has an active genome-sequencing project.

Table 2

Transcriptome resources in forest trees

Family	Genus	Species	Sequenceaccess	Transcriptome	Related publications
Pinaceae	Pinus	taeda (loblolly pine)	[14,19,55]	EST sequencing (Sanger)	[56-59]
			[14,19,60]	EST sequencing (454)	[60]
			[19]	Exome resequencing	[61]
	Pinus	lambertiana (sugar pine)	[14,19,60]	EST sequencing (454)	[60]
	Pseudotsuga	menziesii (Douglas-fir)	[14,19]	EST sequencing (Sanger)
			[14,19,62]	EST sequencing (454)	[60,63,64]
	Pinus	sylvestris (Scots pine)	[14,19,55]	EST sequencing (Sanger)
	Pinus	pinsater (maritime pine)	[14,19,65]	EST sequencing (Sanger/454)	[65,66]
	Pinus	radiata (Monterey pine)	[14,19,55]	EST sequencing (Sanger)	[67-72]
	Picea	abies (Norway spruce)	[14,19,60]	EST sequencing (454)	[60]
			[19]	EST sequencing (Next-Gen)	[73]
	Picea	glauca (white spruce)	[19]	UniGenes (Sanger/454)	[74]
Salicaceae	Populus	trichocarpa (black cottonwood)	[14,19,75]	EST sequencing (Sanger)	[75-78]
			[19]	Exon capture	[79]
			[14,19]	UniGenes (Sanger)	[80]
	Populus	tremula (European aspen)	[14,19,75]	EST sequencing (Sanger)	[75,81]
	Populus	tremula x tremuloides	[14,19,75]	EST sequencing (Sanger)	[75,76]
	Populus	tremuloides (quaking aspen)	[14,19]	EST sequencing (Next-Gen)	[82]
	Populus	nigra (black poplar)	[14,19]	UniGenes (Sanger)	[83]
Myrtaceae	Eucalytpus	grandis (rose gum)	[14,19]	EST sequencing (Sanger)	[84-86]
			[14,19]	EST sequencing (NextGen)	[87]
	Eucalyptus	globulus (blue gum)	[14,19]	EST sequencing (Next-Gen)	[41,88]
	Eucalyptus	camaldulensis (river red gum)	[14,19]	EST sequencing (RNA-Seq)	[89]
Fagaceae	Quercus	robur (English oak)	[19,90]	EST sequencing (454)	[91]
	Castanea	mollissima (Chinese chestnut)	[50]	EST sequencing (454)	[92,93]
Oleaceae	Fraxinus	excelsior (European ash)	[19]	EST sequencing (454)	[94]

Details current transcriptome sequencing projects in forest trees with sequence access information and relevant publications.

Table 3

Polymorphism resources in forest trees

Family	Genus	Species	SNPaccess	Polymorphism	Related Publications
Pinaceae	Pinus	taeda (loblolly pine)	[14,19]	GoldenGate & Infinium iSelect	[95,96]
	Pinus	lambertiana (sugar pine)	[14]	GoldenGate array	[24]
	Pseudotsuga	menziesii (Douglas-fir)	[14]	GoldenGate array	[25]
			[19]	Infinium iSelect	[64]
	Pinus	sylvestris (Scots pine)	[14]	GoldenGate array
	Pinus	pinsater (maritime pine)	[14]	GoldenGate array	[27,90]
	Pinus	radiata (Monterey pine)	[14]	GoldenGate array	[29]
	Picea	abies (Norway spruce)	[14]	GoldenGate array	[30]
	Picea	glauca (white spruce)	[19]	GoldenGate & Infinium iSelect	[97,98]
Salicaceae	Populus	trichocarpa (black cottonwood)	[19,99]	Infinium iSelect	[100]
				Infinium iSelect (Restricted)	[36]
			[14]	GoldenGate array	[101]
			[19,99]	SNP assay	[102]
	Populus	nigra (black poplar)	[14]	GoldenGate array	[103]
Myrtaceae	Eucalytpus	grandis (rose gum)	[104]	DArT high-density array	[105]
			[106]	GoldenGate array	[106]
	Eucalyptus	camaldulensis (river red gum)		RNA-Seq SNP discovery (restricted)	[107]

Details current genotyping projects in forest trees with data access information and relevant publications.

The opportunity for comparative-genomic approaches in forest trees

The power of comparative-genomic approaches for understanding function in an evolutionary framework is well established [7-13]. Comparative genomics can be applied to sequence data (nucleotide and protein) at the level of individual genes or genome-wide. Genome-wide approaches provide insight into both chromosome evolution and the diversification of biological functions and interactions.

Understanding of gene function in forest tree species is challenged by the lack of standard reverse-genetic tools routinely used in other systems - for example, standard marker stocks, facile transformation and regeneration - and by the long generation times. Thus, comparative genomics becomes the more powerful approach to understanding gene function in trees.

Comparative genomics requires not only data availability but also cyber-infrastructure to support exchange and analysis. The TreeGenes database is the most comprehensive resource for comparative-genomic analyses in forest trees [14]. Several smaller databases have been created to facilitate collaborations, including: Fagaceae genomics web, hardwoodgenomics.org, Quercus portal, PineDB, ConiferGDB, EuroPineDB, PopulusDB, PoplarDB, EucalyptusDB and Eucanext (Tables 1, 2, and 3). These resources vary greatly in their scope, relevance and integration. Some are static and archival, whereas others focus on current sequence content for a specific species or a small number of related species. This results in overlapping and conflicting data among repositories. In addition, each database uses its own custom interfaces and back-end database technology to serve sequence to the user. The US National Science Foundation funding for large-scale infrastructure projects, such as iPlant, is leading efforts aimed towards centralizing resources for research communities [15]. Without centralized resources, researchers are forced to employ inefficient data-mining methods through queries of independently maintained databases or inconsistently formatted supplemental files on journal websites. Specific areas of interest for the forest tree genomic community include the ability to connect sequence, genotype and phenotype to individual, geo-referenced trees. This type of integration can only be achieved through web services that allow disparate resources to communicate in ways that are transparent to the user [16]. With the recent increase of genome sequences available for many of these species, there is a need to facilitate community-level annotation and research support.

The need for a better-developed open-access culture in forest tree genomics research

The Human Genome Project established a culture of open access and data sharing in genomics research for both humans and animal models that has been extended to many other species, including Arabidopsis, rat, cow, dog, rice, maize and more than 500 other eukaryotes. Beginning in the late 1990s, these large-scale projects released data very rapidly to the scientific community, often years before publication. This rapid release of data with few restrictions has allowed thousands of scientists to begin work on specific genes and gene families, and on functional studies, long before the genome papers have appeared. One of the driving motivations for this culture, and the reason that many scientists support it, is that large-scale sequencing can be done most efficiently when centers that have expertise in sequencing technology take the lead. With all the sequencing concentrated, the body of data needs to be shared freely in order to get it in the hands of the widely distributed experts. This open-access culture has dramatically accelerated scientific progress in biological research.

The path to success avoids delays

Careful inspection of Table 1 reveals that forest tree genome projects are very slow to release sequence data into the public domain. Once a project is finished and submitted for publication, a draft genome becomes available - for example, the poplar genome was released and published in 2006. However, pre-publication releases are infrequent, exceptions being the PineRefSeq project that has made three releases and the SMarTForest project that has made one (Table 1). This is unfortunate because good-quality sequence contigs and scaffolds could be made available years before publication, delivering an extremely important resource to the community. This delay can be understood from privately financed projects seeking commercial advantages, but nearly all the projects listed in Table 1 are financed by public funds whose stated mission is advancing science and development of community resources. Publication rights are easily protected by data-use policy statements such as the Ft Lauderdale [17] and Toronto agreements [18], but unfortunately these conventions are not often used and data access is restricted by password-protected websites (Tables 1, 2, and 3). We hope the opinion offered here will lead to a discussion in the forest tree community, to a more open-access culture and thus to a more vibrant and rapidly advancing research area.

Box 1

Research priorities in forest tree genomics identified in earlier Opinion papers.

From Neale and Ingvarsson [1]:

•Deep expressed-sequence tag (EST) sequencing in many species

•Comparative resequencing in many species

•Reference genome sequence for pine

From Neale and Kremer [2]:

•Reference genome sequences for several important species

•Greater investment in diverse species towards understanding ecological function

•Application of next-generation sequencing technologies to understand adaptation using landscape genomic approaches

•Greater investment in database resources and cyber-infrastructure development

•Development of new and high-throughput phenotyping technologies

Abbreviations

EST: expressed-sequence tag; Mb: mega-base; NGS: next-generation sequencing.

Competing interests

The authors declare that they have no competing interests.