Shokoofe Noori1, Zuhair M Hassan, Vida Farsam. 1. Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, 1985717443, Iran, shnoori@sbmu.ac.ir.
Abstract
OBJECTIVE: To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line. METHODS: Column chromatography, thin layer chromatography (TLC) and proton NMR spectroscopy were used to purify Artemisinin. The flowcytometry was employed to detect apoptosis and reactive oxygen species (ROS). RESULTS: The results indicated that 50% inhibiting concentration (IC50 value) for pancreatic cell line (RIN) was 45 μmol/L of Artemisinin. Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intracellular ROS. It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover, Artemisinin, in a dose-dependent manner, could significantly increase the level of ROS. CONCLUSION: Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.
OBJECTIVE: To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line. METHODS: Column chromatography, thin layer chromatography (TLC) and proton NMR spectroscopy were used to purify Artemisinin. The flowcytometry was employed to detect apoptosis and reactive oxygen species (ROS). RESULTS: The results indicated that 50% inhibiting concentration (IC50 value) for pancreatic cell line (RIN) was 45 μmol/L of Artemisinin. Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intracellular ROS. It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover, Artemisinin, in a dose-dependent manner, could significantly increase the level of ROS. CONCLUSION:Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.
Authors: R Price; M van Vugt; F Nosten; C Luxemburger; A Brockman; L Phaipun; T Chongsuphajaisiddhi; N White Journal: Am J Trop Med Hyg Date: 1998-12 Impact factor: 2.345