| Literature DB >> 23757041 |
Dongli Xu1, Tao Jiang, Anan Li, Bihe Hu, Zhao Feng, Hui Gong, Shaoqun Zeng, Qingming Luo.
Abstract
High-throughput optical imaging is critical to obtain large-scale neural connectivity information of brain in neuroscience. Using a digital mirror device and a scientific complementary metal-oxide semiconductor camera, we report a significant speed improvement of structured illumination microscopy (SIM), which produces a maximum SIM net frame rate of 133 Hz. We perform three-dimensional (3-D) imaging of mouse brain slices at diffraction-limited resolution and demonstrate the fast 3-D imaging capability to a large sample with an imaging rate of 6.9×10(7) pixel/s of our system, an order of magnitude faster than previously reported.Entities:
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Year: 2013 PMID： 23757041 DOI： 10.1117/1.JBO.18.6.060503
Source DB: PubMed Journal: J Biomed Opt ISSN： 1083-3668 Impact factor: 3.170