Literature DB >> 23748699

Investigation of tryptophan-NADH interactions in live human cells using three-photon fluorescence lifetime imaging and Förster resonance energy transfer microscopy.

Vinod Jyothikumar, Yuansheng Sun, Ammasi Periasamy.   

Abstract

A method to investigate the metabolic activity of intracellular tryptophan (TRP) and coenzyme-NADH using three-photon (3P) fluorescence lifetime imaging (FLIM) and Förster resonance energy transfer (FRET) is presented. Through systematic analysis of FLIM data from tumorigenic and nontumorigenic cells, a statistically significant decrease in the fluorescence lifetime of TRP was observed in response to the increase in protein-bound NADH as cells were treated with glucose. The results demonstrate the potential use of 3P-FLIM-FRET as a tool for label-free screening of the change in metabolic flux occurring in human diseases or other clinical conditions.

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Year:  2013        PMID： 23748699      PMCID： PMC3675329          DOI： 10.1117/1.JBO.18.6.060501

Source DB:  PubMed          Journal:  J Biomed Opt        ISSN： 1083-3668            Impact factor:   3.170


  9 in total

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Journal:  Cancer Res       Date:  2005-10-01       Impact factor: 12.701

3.  Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy.

Authors:  Yuansheng Sun; Richard N Day; Ammasi Periasamy
Journal:  Nat Protoc       Date:  2011-08-11       Impact factor: 13.491

4.  Lifetimes and NADH quenching of tryptophan fluorescence in pig heart lactate dehydrogenase.

Authors:  T Torikata; L S Forster; C C O'Neal; J A Rupley
Journal:  Biochemistry       Date:  1979-01-23       Impact factor: 3.162

5.  Characterization of the autofluorescence of polymorphonuclear leukocytes, mononuclear leukocytes and cervical epithelial cancer cells for improved spectroscopic discrimination of inflammation from dysplasia.

Authors:  D L Heintzelman; R Lotan; R R Richards-Kortum
Journal:  Photochem Photobiol       Date:  2000-03       Impact factor: 3.421

Review 6.  Energy metabolism in tumor cells.

Authors:  Rafael Moreno-Sánchez; Sara Rodríguez-Enríquez; Alvaro Marín-Hernández; Emma Saavedra
Journal:  FEBS J       Date:  2007-03       Impact factor: 5.542

7.  Measuring serotonin distribution in live cells with three-photon excitation.

Authors:  S Maiti; J B Shear; R M Williams; W R Zipfel; W W Webb
Journal:  Science       Date:  1997-01-24       Impact factor: 47.728

8.  Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the single-cell level.

Authors:  Qianru Yu; Ahmed A Heikal
Journal:  J Photochem Photobiol B       Date:  2008-12-25       Impact factor: 6.252

9.  In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia.

Authors:  Melissa C Skala; Kristin M Riching; Damian K Bird; Annette Gendron-Fitzpatrick; Jens Eickhoff; Kevin W Eliceiri; Patricia J Keely; Nirmala Ramanujam
Journal:  J Biomed Opt       Date:  2007 Mar-Apr       Impact factor: 3.170
  9 in total
  7 in total

Review 1.  Recent trends in two-photon auto-fluorescence lifetime imaging (2P-FLIM) and its biomedical applications.

Authors:  Harsh Ranawat; Sagnik Pal; Nirmal Mazumder
Journal:  Biomed Eng Lett       Date:  2019-07-01

2.  Detection of urinary bladder cancer cells using redox ratio and double excitation wavelengths autofluorescence.

Authors:  Scott Palmer; Karina Litvinova; Edik U Rafailov; Ghulam Nabi
Journal:  Biomed Opt Express       Date:  2015-02-25       Impact factor: 3.732

3.  Structure, Function, and Thermodynamics of Lactate Dehydrogenases from Humans and the Malaria Parasite P. falciparum.

Authors:  Sergei Khrapunov; Akiba Waterman; Rudra Persaud; Eric P Chang
Journal:  Biochemistry       Date:  2021-11-08       Impact factor: 3.162

4.  Segmented cell analyses to measure redox states of autofluorescent NAD(P)H, FAD & Trp in cancer cells by FLIM.

Authors:  Horst Wallrabe; Zdenek Svindrych; Shagufta R Alam; Karsten H Siller; Tianxiong Wang; David Kashatus; Song Hu; Ammasi Periasamy
Journal:  Sci Rep       Date:  2018-01-08       Impact factor: 4.379

5.  Investigation of Mitochondrial Metabolic Response to Doxorubicin in Prostate Cancer Cells: An NADH, FAD and Tryptophan FLIM Assay.

Authors:  Shagufta Rehman Alam; Horst Wallrabe; Zdenek Svindrych; Ajay K Chaudhary; Kathryn G Christopher; Dhyan Chandra; Ammasi Periasamy
Journal:  Sci Rep       Date:  2017-09-05       Impact factor: 4.379

6.  Special Section Guest Editorial: Thirty Years of Multiphoton Microscopy in the Biomedical Sciences.

Authors:  Ammasi Periasamy; Karsten König; Peter So
Journal:  J Biomed Opt       Date:  2020-01       Impact factor: 3.170

Review 7.  Intrinsic tryptophan fluorescence in the detection and analysis of proteins: a focus on Förster resonance energy transfer techniques.

Authors:  Amar B T Ghisaidoobe; Sang J Chung
Journal:  Int J Mol Sci       Date:  2014-12-05       Impact factor: 5.923
  7 in total

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