Literature DB >> 8999797

Measuring serotonin distribution in live cells with three-photon excitation.

S Maiti1, J B Shear, R M Williams, W R Zipfel, W W Webb.   

Abstract

Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established by 3PE cross section measurements of tryptophan and the monoamines serotonin and dopamine, circumvents the limitations imposed by photodamage, scattering, and indiscriminate background encountered in other UV microscopies. Three-dimensionally resolved images are presented along with measurements of the serotonin concentration ( approximately 50 mM) and content (up to approximately 5 x 10(8) molecules) of individual secretory granules.

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Year:  1997        PMID： 8999797     DOI： 10.1126/science.275.5299.530

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  71 in total

1.  High-resolution nonlinear optical imaging of live cells by second harmonic generation.

Authors:  P J Campagnola; M D Wei; A Lewis; L M Loew
Journal:  Biophys J       Date:  1999-12       Impact factor: 4.033

2.  Coherent scattering in multi-harmonic light microscopy.

Authors:  L Moreaux; O Sandre; S Charpak; M Blanchard-Desce; J Mertz
Journal:  Biophys J       Date:  2001-03       Impact factor: 4.033

Review 3.  Evanescent-wave microscopy: a new tool to gain insight into the control of transmitter release.

Authors:  M Oheim; D Loerke; R H Chow; W Stühmer
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1999-02-28       Impact factor: 6.237

4.  Characterization of single-cell electroporation by using patch-clamp and fluorescence microscopy.

Authors:  F Ryttsén; C Farre; C Brennan; S G Weber; K Nolkrantz; K Jardemark; D T Chiu; O Orwar
Journal:  Biophys J       Date:  2000-10       Impact factor: 4.033

5.  Robust, high-resolution, whole cell patch-clamp capacitance measurements using square wave stimulation.

Authors:  R E Thompson; M Lindau; W W Webb
Journal:  Biophys J       Date:  2001-08       Impact factor: 4.033

6.  Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation.

Authors:  Warren R Zipfel; Rebecca M Williams; Richard Christie; Alexander Yu Nikitin; Bradley T Hyman; Watt W Webb
Journal:  Proc Natl Acad Sci U S A       Date:  2003-05-19       Impact factor: 11.205

7.  Nondiffusive mechanisms enhance protein uptake rates in ion exchange particles.

Authors:  S R Dziennik; E B Belcher; G A Barker; M J DeBergalis; S E Fernandez; A M Lenhoff
Journal:  Proc Natl Acad Sci U S A       Date:  2003-01-08       Impact factor: 11.205

8.  Multiphoton-excited serotonin photochemistry.

Authors:  Michael L Gostkowski; Richard Allen; Matthew L Plenert; Eric Okerberg; Mary Jane Gordon; Jason B Shear
Journal:  Biophys J       Date:  2004-05       Impact factor: 4.033

9.  Nanoscale spatial induction of ultraviolet photoproducts in cellular DNA by three-photon near-infrared absorption.

Authors:  Rosalind A Meldrum; Stanley W Botchway; Christopher W Wharton; Graeme J Hirst
Journal:  EMBO Rep       Date:  2003-11-14       Impact factor: 8.807

10.  Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes.

Authors:  J A Lundqvist; F Sahlin; M A Aberg; A Strömberg; P S Eriksson; O Orwar
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205
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